Journal ArticleDOI
Nutrition needs of mammalian cells in tissue culture.
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This article is published in Science.The article was published on 1955-09-16. It has received 1462 citations till now. The article focuses on the topics: Blood serum & Blood chemistry.read more
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Regulation of cancer cell metabolism
TL;DR: Interest in the topic of tumour metabolism has waxed and waned over the past century, but it has become clear that many of the signalling pathways that are affected by genetic mutations and the tumour microenvironment have a profound effect on core metabolism, making this topic once again one of the most intense areas of research in cancer biology.
Journal ArticleDOI
Amino Acid Metabolism in Mammalian Cell Cultures
TL;DR: The present article "is a progress report rather than a review and in large part summarizes studies from a single laboratory" on the minimal essential medium for cultivation of mammalian cells in either monolayer or suspension.
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Ferroptosis: A Regulated Cell Death Nexus Linking Metabolism, Redox Biology, and Disease
Brent R. Stockwell,José Pedro Friedmann Angeli,Hülya Bayır,Ashley I. Bush,Marcus Conrad,Scott J. Dixon,Simone Fulda,Susan Gascon,Stavroula K. Hatzios,Valerian E. Kagan,Kay Noel,Xuejun Jiang,Andreas Linkermann,Maureen E. Murphy,Michael Overholtzer,Atsushi Oyagi,Gabriela Carolina Pagnussat,Jason Park,Qitao Ran,Craig S. Rosenfeld,Konstantin Salnikow,Daolin Tang,Daolin Tang,Frank M. Torti,Suzy V. Torti,Shinya Toyokuni,K. A. Woerpel,Donna D. Zhang +27 more
TL;DR: The mechanisms underlying ferroptosis are reviewed, connections to other areas of biology and medicine are highlighted, and tools and guidelines for studying this emerging form of regulated cell death are recommended.
Journal ArticleDOI
Myc regulates a transcriptional program that stimulates mitochondrial glutaminolysis and leads to glutamine addiction
David R. Wise,Ralph J. DeBerardinis,Anthony A. Mancuso,Nabil Sayed,Xiao-yong Zhang,Harla K. Pfeiffer,Ilana Nissim,Evgueni Daikhin,Marc Yudkoff,Steven B. McMahon,Craig B. Thompson +10 more
TL;DR: It is reported that the transcriptional regulatory properties of the oncogene Myc coordinate the expression of genes necessary for cells to engage in glutamine catabolism that exceeds the cellular requirement for protein and nucleotide biosynthesis, resulting in the reprogramming of mitochondrial metabolism to depend on glutaminolysis to sustain cellular viability and TCA cycle anapleurosis.
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Glutamine addiction: a new therapeutic target in cancer.
David R. Wise,Craig B. Thompson +1 more
TL;DR: In many cancer cells, glutamine is the primary mitochondrial substrate and is required for maintenance of mitochondrial membrane potential and integrity and for support of the NADPH production needed for redox control and macromolecular synthesis.
References
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The free amino acids of human blood plasma.
William H. Stein,Stanford Moore +1 more
TL;DR: It has been possible to identify with a high degree of probability twenty-eight ninhydrin-positive substances in protein-free plasma and to determine most of them quantitatively.
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Studies on the propagation in vitro of poliomyelitis viruses. IV. Viral multiplication in a stable strain of human malignant epithelial cells (strain HeLa) derived from an epidermoid carcinoma of the cervix.
TL;DR: The growth of poliomyelitis virus, Type 2, Yale-SK strain, in cultures of monkey testicular tissue was observed to occur in discrete cycles, and the shape of the growth curve as established by any of four different techniques for tissue cultivation, was shown to be independent of the cultural technique employed.
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The growth in vitro of single isolated tissue cells.
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Propagation in a fluid medium of a human epidermoid carcinoma, strain KB.
TL;DR: An epidermoid carcinoma of the floor of the mouth was cultivated directly onto a glass surface in a medium consisting of 13 amino acids, 7 vitamins, glucose, salts, and 10% human serum, and all were used at concentrations permitting maximal growth of that cell.
Journal ArticleDOI
The growth response of mammalian cells in tissue culture to L-glutamine and L-glutamic acid.
TL;DR: In a medium containing the twelve amino acids previously shown to be essential, the seven demonstrably essential vitamins, glucose, electrolytes, and serum protein, both the mouse fibroblast and the human carcinoma cell degenerated and died unless the medium was supplemented with glutamine.