Journal ArticleDOI
Optimization of chemically defined medium for recombinant Pichia pastoris for biomass production
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TLDR
The notion that P. pastoris cells grow on glycerol by a respiratory route and are therefore an efficient biomass and protein producers is supported.About:
This article is published in Bioresource Technology.The article was published on 2008-11-01. It has received 56 citations till now. The article focuses on the topics: Pichia pastoris & Chemostat.read more
Citations
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Enhancement of cell viability and alkaline polygalacturonate lyase production by sorbitol co-feeding with methanol in Pichia pastoris fermentation.
TL;DR: Results revealed that an appropriate sorbitol co-feeding strategy not only decreased the cell mortality in the end of fermentation, but also reduced the proteolytic degradation of PGL.
Journal ArticleDOI
Effects of abiotic stressors on lutein production in the green microalga Dunaliella salina
Weiqi Fu,Giuseppe Paglia,Manuela Magnusdottir,Elín A Steinarsdóttir,Steinn Gudmundsson,Bernhard O. Palsson,Bernhard O. Palsson,Ólafur S. Andrésson,Sigurður Brynjólfsson +8 more
TL;DR: This study shows that systematic evaluation of the relationship between abiotic environmental stresses and lutein biosynthesis can help to decipher the key parameters in obtaining high levels of lutenin productivity in D. salina.
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Pichia pastoris: a recombinant microfactory for antibodies and human membrane proteins.
A. M. Gonçalves,Augusto Q. Pedro,Cláudio J. Maia,Fani Sousa,João A. Queiroz,Luís A. Passarinha +5 more
TL;DR: The main aim is to highlight the relevance and versatility of the P. pastoris biosystem to the design of more cost-effective microfactories to meet the increasing demands for recombinant membrane proteins and clinical antibodies for several therapeutic applications.
Journal ArticleDOI
GlycoFi's technology to control the glycosylation of recombinant therapeutic proteins.
TL;DR: This opinion paper is to present a comprehensive overview of the technological breakthrough developed by GlycoFi to produce recombinant human proteins with controlled glycosylation patterns in yeast, in comparison to other glyco-engineering technologies and to discuss the application to large-scale manufacturing of biologicals.
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Optimization of medium composition for butyric acid production by Clostridium thermobutyricum using response surface methodology.
TL;DR: The optimal medium for butyric acid production by Clostridium thermobutyricum in a shake flask culture was studied using statistical experimental design and analysis and the high correlation between the predicted and observed values indicated the validity of the model.
References
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Journal ArticleDOI
The Growth of Bacterial Cultures
TL;DR: Bacterial growth is considered as a method for the study of bacterial physiology and biochemistry, with the interpretation of quantitative data referring to bacterial growth limited to populations considered genetically homogeneous.
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Some New Three Level Designs for the Study of Quantitative Variables
George E. P. Box,D. W. Behnken +1 more
TL;DR: In this paper, a class of incomplete three level factorial designs useful for estimating the coefficients in a second degree graduating polynomial are described and the designs either meet, or approximately meet, the criterion of rotatability and for the most part can be orthogonally blocked.
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Multi-Factor Experimental Designs for Exploring Response Surfaces
George E. P. Box,J. S. Hunter +1 more
TL;DR: In this paper, the concept of the variance function for an experimental design is introduced, and the problem of selecting practically useful designs is discussed, and in this connection, the notion of variance function is introduced.
Journal ArticleDOI
Production of recombinant proteins in fermenter cultures of the yeast Pichia pastoris.
TL;DR: The Pichia pastoris expression system offers economy, ease of manipulation, the ability to perform complex post-translational modifications, and high expression levels.
Journal ArticleDOI
Use of response surface methodology for optimizing process parameters for the production of α-amylase by Aspergillus oryzae
Febe Francis,Febe Francis,A. Sabu,K. Madhavan Nampoothiri,Sumitra Ramachandran,Sanjoy Ghosh,George Szakacs,Ashok Pandey +7 more
TL;DR: In this article, the optimal production of α-amylase by Aspergillus oryzae NRRL 6270 in solid-state fermentation (SSF) was attempted by using a Box-Behnken design under the response surface methodology.