OsPT2, a phosphate transporter, is involved in the active uptake of selenite in rice
Lianhe Zhang,Bin Hu,Wei Li,Ronghui Che,Kun Deng,Hua Li,Feiyan Yu,Hong-Qing Ling,Youjun Li,Chengcai Chu +9 more
TLDR
Data strongly demonstrate that selenite and Pi share similar uptake mechanisms and that OsPT2 is involved in selenites uptake, which provides a potential strategy for breeding Se-enriched rice varieties.Abstract:
• Selenite is a predominant form of selenium (Se) available to plants, especially in anaerobic soils, but the molecular mechanism of selenite uptake by plants is not well understood. • ltn1, a rice mutant previously shown to have increased phosphate (Pi) uptake, was found to exhibit higher selenite uptake than the wild-type in both concentration- and time-dependent selenite uptake assays. Respiratory inhibitors significantly inhibited selenite uptake in the wildtype and the ltn1 mutant, indicating that selenite uptake was coupled with H(+) and energy-dependent. Selenite uptake was greatly enhanced under Pi-starvation conditions, suggesting that Pi transporters are involved in selenite uptake. • OsPT2, the most abundantly expressed Pi transporter in the roots, is also significantly up-regulated in ltn1 and dramatically induced by Pi starvation. OsPT2-overexpressing and knockdown plants displayed significantly increased and decreased rates of selenite uptake, respectively, suggesting that OsPT2 plays a crucial role in selenite uptake. Se content in rice grains also increased significantly in OsPT2-overexpressing plants. • These data strongly demonstrate that selenite and Pi share similar uptake mechanisms and that OsPT2 is involved in selenite uptake, which provides a potential strategy for breeding Se-enriched rice varieties.read more
Citations
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Journal ArticleDOI
Selenium Cycling Across Soil-Plant-Atmosphere Interfaces: A Critical Review
Lenny H. E. Winkel,Bas Vriens,Gerrad D. Jones,Leila S. Schneider,Elizabeth A. H. Pilon-Smits,Gary S. Bañuelos +5 more
TL;DR: An overview of current knowledge on Se cycling with a specific focus on soil-plant-atmosphere interfaces is given and sources, speciation and mobility of Se in soils and plants will be discussed as well as Se hyperaccumulation by plants, biofortification and biomethylation.
Journal ArticleDOI
Selenium accumulation by plants
TL;DR: The ability to tolerate large tissue Se concentrations is primarily related to the ability to divert Se away from the accumulation of selenocysteine and selenomethionine, which might be incorporated into non-functional proteins, through the synthesis of less toxic Se metabilites.
Journal ArticleDOI
A critical review of selenium biogeochemical behavior in soil-plant system with an inference to human health.
Natasha,Muhammad Shahid,Nabeel Khan Niazi,Nabeel Khan Niazi,Nabeel Khan Niazi,Sana Khalid,Behzad Murtaza,Irshad Bibi,Muhammad Rashid +8 more
TL;DR: This review traces a plausible link among Se levels, sources, speciation, bioavailability, and effect of soil chemical properties on selenium bioavailability/speciation in soil; role of different protein transporters in soil-root-shoot transfer of Se; and the potential role of Se in different human disorders/diseases.
Journal ArticleDOI
The fascinating facets of plant selenium accumulation - biochemistry, physiology, evolution and ecology.
TL;DR: The importance of selenium (Se) for medicine, industry and the environment is increasingly apparent, and mechanisms of Se accumulation and tolerance in plants and algae, Se hyperaccumulation, and ecological and evolutionary aspects of these processes are discussed.
Journal ArticleDOI
Complex Regulation of Plant Phosphate Transporters and the Gap between Molecular Mechanisms and Practical Application: What Is Missing?
TL;DR: Here, an increasing number of yeast PHO84 homologs as well as other genes encoding proteins with phosphate (Pi) transport activities have been identified and functionally characterized in diverse plant species and potential strategies for effective use of PTs are proposed in improving plant growth and development.
References
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Journal Article
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