Palmitoylation of the human beta 2-adrenergic receptor. Mutation of Cys341 in the carboxyl tail leads to an uncoupled nonpalmitoylated form of the receptor.
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TLDR
Results indicate that post-translational modification by palmitate of beta 2AR may play a crucial role in the normal coupling of the receptor to the adenylyl cyclase signal transduction system.About:
This article is published in Journal of Biological Chemistry.The article was published on 1989-05-05 and is currently open access. It has received 589 citations till now. The article focuses on the topics: 5-HT5A receptor & Beta-2 adrenergic receptor.read more
Citations
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A novel multigene family may encode odorant receptors: A molecular basis for odor recognition
Linda B. Buck,Richard Axel +1 more
TL;DR: This work has cloned and characterized 18 different members of an extremely large multigene family that encodes seven transmembrane domain proteins whose expression is restricted to the olfactory epithelium and is likely to encode a diverse family of odorant receptors.
Journal ArticleDOI
Dopamine Receptors: From Structure to Function
TL;DR: Target deletion of several of these dopamine receptor genes in mice should provide valuable information about their physiological functions and provide unequivocal evidence for the involvement of one of these receptors in the etiology of various central nervous system disorders.
Journal ArticleDOI
High-Resolution Crystal Structure of an Engineered Human β2-Adrenergic G Protein–Coupled Receptor
Vadim Cherezov,Daniel M. Rosenbaum,Michael A. Hanson,Søren G. F. Rasmussen,Foon Sun Thian,Tong Sun Kobilka,Hee Jung Choi,Peter Kuhn,William I. Weis,Brian K. Kobilka,Raymond C. Stevens +10 more
TL;DR: Although the location of carazolol in the β2-adrenergic receptor is very similar to that of retinal in rhodopsin, structural differences in the ligand-binding site and other regions highlight the challenges in using rhodopin as a template model for this large receptor family.
Journal ArticleDOI
Cloning and expression of a cDNA encoding an endothelin receptor
TL;DR: The cloning of a complementary DNA encoding a bovine endothelin receptor is reported, which has a transmembrane topology similar to that of other G protein-coupled receptors and shows specific binding, with the highest selectivity to ET-1 in animal cells trans-fected with the cloned cDNA.
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Cloning of the gene for a human dopamine D4 receptor with high affinity for the antipsychotic clozapine.
Hubert H.M. Van Tol,James R. Bunzow,Hong-Chang Guan,Roger K. Sunahara,Philip Seeman,Hyman B. Niznik,Olivier Civelli +6 more
TL;DR: The cloning of a gene that encodes a dopamine receptor gene that has high homology to the human dopamine D2 and D3 receptor genes is reported, which suggests the existence of other types of dopamine receptors which are more sensitive to clozapine.
References
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Journal ArticleDOI
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
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A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
Journal ArticleDOI
Ligand: a versatile computerized approach for characterization of ligand-binding systems.
Peter J. Munson,David Rodbard +1 more
TL;DR: This approach provides two major advantages compared with other available methods: it uses an exact mathematical model of the ligand-binding system, thereby avoiding the possible biases introduced by several commonly used approximations and it uses a statistically valid, appropriately weighted least-squares curve-fitting algorithm with objective measurement of goodness of fit.
Journal ArticleDOI
A ternary complex model explains the agonist-specific binding properties of the adenylate cyclase-coupled beta-adrenergic receptor.
TL;DR: In this paper, a ternary complex model was used to fit the data with high accuracy under conditions where the ligand used is either a full or a partial agonist and where the system is altered by the addition of guanine nucleotide or after treatment with group-specific reagents.
Book ChapterDOI
Use of eukaryotic expression technology in the functional analysis of cloned genes
TL;DR: The purpose of this chapter is to describe ways in which eukaryotic expression technology can be used to identify and to analyze the function of cloned eukARYotic genes.