Phasic Neuronal Firing in the Rodent Nucleus of the Solitary Tract ex vivo.
Lukasz Chrobok,Michal Wojcik,Jasmin Daniela Klich,Kamil Pradel,Marian H. Lewandowski,Hugh D. Piggins +5 more
TLDR
In this paper, the authors used multi-electrode array recordings to study the firing pattern of neurons in the nucleus of the solitary tract (NTS) and identified it as a potential downstream target of the noradrenergic system.Abstract:
Phasic pattern of neuronal activity has been previously described in detail for magnocellular vasopressin neurons in the hypothalamic paraventricular and supraoptic nuclei. This characteristic bistable pattern consists of alternating periods of electrical silence and elevated neuronal firing, implicated in neuropeptide release. Here, with the use of multi-electrode array recordings ex vivo, we aimed to study the firing pattern of neurons in the nucleus of the solitary tract (NTS) - the brainstem hub for homeostatic, cardio-vascular, and metabolic processes. Our recordings from the mouse and rat hindbrain slices reveal the phasic activity pattern to be displayed by a subset of neurons in the dorsomedial NTS subjacent to the area postrema (AP), with the inter-spike interval distribution closely resembling that reported for phasic magnocellular vasopressin cells. Additionally, we provide interspecies comparison, showing higher phasic frequency and firing rate of phasic NTS cells in mice compared to rats. Further, we describe daily changes in their firing rate and pattern, peaking at the middle of the night. Last, we reveal these phasic cells to be sensitive to α 2 adrenergic receptors activation and to respond to electrical stimulation of the AP. This study provides a comprehensive description of the phasic neuronal activity in the rodent NTS and identifies it as a potential downstream target of the AP noradrenergic system.read more
Citations
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Journal ArticleDOI
Rhythmic neuronal activities of the rat nucleus of the solitary tract are impaired by high-fat diet - implications for daily control of satiety.
Lukasz Chrobok,Jasmin Daniela Klich,Anna M Sanetra,Jagoda Stanislawa Jeczmien-Lazur,Kamil Pradel,Katarzyna Palus-Chramiec,Mariusz Kepczynski,Hugh D Piggins,Marian H. Lewandowski +8 more
TL;DR: In this paper, the authors used a combination of immunohistochemical and electrophysiological approaches together with daily monitoring of body weight and food intake to interrogate how the neuronal rhythms of the dorsal vagal complex of the brainstem are affected by a high-fat diet.
Journal ArticleDOI
Daily changes in neuronal activities of the dorsal motor nucleus of the vagus under standard and high-fat diet
Lukasz Chrobok,Jasmin Daniela Klich,Jagoda Stanislawa Jeczmien-Lazur,Kamil Pradel,Katarzyna Palus-Chramiec,Anna M Sanetra,Hugh D Piggins,Marian H. Lewandowski +7 more
TL;DR: In this article, the authors used a combination of multi-channel electrophysiology and patch clamp recordings to gain insight into effects of time of day and diet on these DMV cells.
Posted ContentDOI
Intrinsic circadian timekeeping properties of the thalamic lateral geniculate nucleus
Lukasz Chrobok,Kamil Pradel,Marcelina Janik,Anna M Sanetra,Monika Bubka,Jihwan Myung,Amalia Ridla Rahim,Jasmin Daniela Klich,Jagoda Stanislawa Jeczmien-Lazur,Katarzyna Palus-Chramiec,Marian H. Lewandowski +10 more
TL;DR: In this article, a combination of molecular, electrophysiological, and optogenetic tools were used to evaluate intrinsic clock properties of the lateral geniculate nucleus (LGN) in male rats and mice.
Journal ArticleDOI
Vasopressin and Breathing: Review of Evidence for Respiratory Effects of the Antidiuretic Hormone
Michał Proczka,Jacek Przybylski,Agnieszka Cudnoch-Jędrzejewska,Ewa Szczepanska-Sadowska,Tymoteusz Żera +4 more
TL;DR: Proczka et al. as mentioned in this paper showed that increased vasopressin release and changes in ventilation are not coincidental, but that the neurohormone contributes to the regulation of the respiratory system by fine-tuning of breathing in order to restore homeostasis.
Journal ArticleDOI
Racing and Pacing in the Reward System: A Multi-Clock Circadian Control Over Dopaminergic Signalling
TL;DR: The dopaminergic system is placed as a hub in the extensive network of extra-SCN circadian oscillators and the possible consequences of its daily entrainment for animal physiology and behaviour are discussed.
References
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Journal ArticleDOI
The central neural connections of the area postrema of the rat.
TL;DR: These connections, when considered in the context of the known vagal afferent input and reduced blood‐brain barrier of AP, place this structure in a unique position to receive and modulate ascending interoceptive information and to influence autonomic outflow as well.
Journal ArticleDOI
Hindbrain neurons as an essential hub in the neuroanatomically distributed control of energy balance.
Harvey J. Grill,Matthew R. Hayes +1 more
TL;DR: This Review highlights the processing and integration performed by hindbrain nuclei, focusing on the inputs received by nucleus tractus solitarius (NTS) neurons, and proposes that NTS (and hindbrain neurons, more broadly) integrate these multiple energy status signals and issue-output commands controlling the behavioral, autonomic, and endocrine responses that collectively govern energy balance.
Journal ArticleDOI
Phasic firing enhances vasopressin release from the rat neurohypophysis.
A. Dutton,R. E. J. Dyball +1 more
TL;DR: Isolated rat neural lobes were incubated in vitro and electrically stimulated to release vasopressin and there was a reasonably good correlation between results obtained with this assay and those obtained by bioassay with the rat blood pressure method.
Journal ArticleDOI
Experimental study of the projections of the nucleus of the tractus solitarius and the area postrema in the cat.
TL;DR: Evidence is given to support the hypothesis that ascening visceral pathways are interruped in the bulbar reticular formation and dorsal tegmental nucleus before reaching the diencephalon as well as with functional aspects of the central autonomic and reticular activating systems.
Journal ArticleDOI
The area postrema and vomiting.
Alan D. Miller,Ronald A. Leslie +1 more
TL;DR: The area postrema (AP) has been implicated as a chemoreceptor trigger zone for vomiting (emesis) for over 40 years and is anatomically positioned to detect emetic toxins in the blood as well as in the CSF.
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