“Ping-Pong” Interactions between Mitochondrial tRNA Import Receptors within a Multiprotein Complex
Subhendra Nath Bhattacharyya,Saibal Chatterjee,Srikanta Goswami,Gayatri Tripathi,Sailendra Nath Dey,Samit Adhya +5 more
TLDR
By a combination of antibody inhibition, photochemical cross-linking, and immunoprecipitation, it was shown that binding of tRNAIle to a 21-kDa component of the complex is dependent upon tRNATyr, whilebinding of tR NATyr to a 45-KDa component is inhibited by t RNAIle, suggesting this “ping-pong” mechanism may be an effective means to maintain a balanced tRNA pool for mitochondrial translation.Abstract:
The mitochondrial genomes of a wide variety of species contain an insufficient number of functional tRNA genes, and translation of mitochondrial mRNAs is sustained by import of nucleus-encoded tRNAs. In Leishmania, transfer of tRNAs across the inner membrane can be regulated by positive and negative interactions between them. To define the factors involved in such interactions, a large multisubunit complex (molecular mass, approximately 640 kDa) from the inner mitochondrial membrane of the kinetoplastid protozoon Leishmania, consisting of approximately 130-A particles, was isolated. The complex, when incorporated into phospholipid vesicles, induced specific, ATP- and proton motive force-dependent transfer of Leishmania tRNA(Tyr) as well as of oligoribonucleotides containing the import signal YGGYAGAGC. Moreover, allosteric interactions between tRNA(Tyr) and tRNA(Ile) were observed in the RNA import complex-reconstituted system, indicating the presence of primary and secondary tRNA binding sites within the complex. By a combination of antibody inhibition, photochemical cross-linking, and immunoprecipitation, it was shown that binding of tRNA(Ile) to a 21-kDa component of the complex is dependent upon tRNA(Tyr), while binding of tRNA(Tyr) to a 45-kDa component is inhibited by tRNA(Ile). This "ping-pong" mechanism may be an effective means to maintain a balanced tRNA pool for mitochondrial translation.read more
Citations
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Journal ArticleDOI
Mitochondrial differentiation in kinetoplastid protozoa: a plethora of RNA controls
Samit Adhya,Sudarshana Basu,Suvendra N. Bhattacharyya,Saibal Chatterjee,Gunjan Dhar,Srikanta Goswami,Sanjay Ghosh,Pratik Home,Bidesh Mahata,Gayatri Tripathi +9 more
TL;DR: Multilevel post-transcriptional regulatory mechanisms by which the expression of the nuclear and mitochondrially encoded components of respiratory enzymes is coordinated are revealed, as well as the identities of some general and gene-specific factors controlling mitochondrial differentiation.
Patent
Protein-coding RNA to correct mitochondrial dysfunction
TL;DR: In this paper, the authors present compositions and methods for using correctional RNA, with a proteinaceous carrier, to stimulate respiration of the cells, tissues, organs or the whole organism of normal or diseased subjects.
Book ChapterDOI
Oxidative Stress and its Biochemical Consequences in Mitochondrial DNA Mutation-Associated Diseases: Implications of Redox Therapy for Mitochondrial Diseases
Dissertation
Mitochondrial Biology: RNA Import and Xenomitochondrial Compensatory Mechanisms
TL;DR: This book discusses mitochondrial biology, animal models of mitochondrial disease, and compensatory mechanisms in xenomitochondrial mice.
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