Plasma kinetics in man of epicatechin from black chocolate

TLDR: Epicatechin is absorbed from chocolate and is rapidly eliminated from plasma and the maximal concentration and area under the curve of plasma kinetics of both substrates correlated very well with the dose of chocolate.

Abstract: Objective: To evaluate the plasma kinetics in man of epicatechin from black chocolate. Design: An intervention study with 8 volunteers. Each served as his own control. Theobromine was used as control marker of the chocolate intake. Setting: Metabolic Unit, Nestle Research Center, Vers-chez-les-Blanc, Switzerland. Subjects: Eight healthy male volunteers (4 smokers and 4 non-smokers) were enrolled in this study. They abstained from foods rich in polyphenols (coffee, tea, wine, fruit juice, cocoa products) for 24 h prior to the test until its completion. Intervention: Volunteers ate 40 g and 80 g of black chocolate (Nestle Noir) together with bread with a one-week interval. Blood samples were drawn every hour during the first 4 h and a last one at 8 h after chocolate consumption. Plasma samples were analysed for epicatechin and theobromine content by HPLC. Results: Plasma concentrations of epicatechin and theobromine increased markedly after chocolate consumption (P=0.002 and P=0.001, respectively), reaching a maximum between 2 and 3 h. The maximal concentration and area under the curve of plasma kinetics of both substrates correlated very well with the dose of chocolate. Conclusions: Epicatechin is absorbed from chocolate and is rapidly eliminated from plasma. Attainable plasma values are 0.7 μmol/l from 80 g of black chocolate. Sponshorship: Nestec LTD, Vevey, Switzerland.