Pluripotency state regulates cytoneme selectivity and self-organization of embryonic stem cells.
Reads0
Chats0
TLDR
The authors showed that ESC transition to an early primed ESC (pESC) state reduces their pairing with trophoblast stem cells and impairs synthetic embryogenesis, due to an impaired crosstalk between Wnt and glutamate receptor activity and reduced generation of Ca2+ transients on the cytonemes upon Wnt source contact.Abstract:
To coordinate cell fate with changes in spatial organization, stem cells (SCs) require specific and adaptable systems of signal exchange and cell-to-cell communication. Pluripotent embryonic stem cells (ESCs) use cytonemes to pair with trophoblast stem cells (TSCs) and form synthetic embryonic structures in a Wnt-dependent manner. How these interactions vary with pluripotency states remains elusive. Here we show that ESC transition to an early primed ESC (pESC) state reduces their pairing with TSCs and impairs synthetic embryogenesis. pESCs can activate the Wnt/β-catenin pathway in response to soluble Wnt ligands, but their cytonemes form unspecific and unstable interactions with localized Wnt sources. This is due to an impaired crosstalk between Wnt and glutamate receptor activity and reduced generation of Ca2+ transients on the cytonemes upon Wnt source contact. Induced iGluR activation can partially restore cytoneme function in pESCs, while transient overexpression of E-cadherin improves pESC-TSC pairing. Our results illustrate how changes in pluripotency state alter the mechanisms SCs use to self-organize.read more
Citations
More filters
Journal ArticleDOI
Joint single-cell multiomic analysis in Wnt3a induced asymmetric stem cell division.
TL;DR: In this paper, the same cell epigenome and transcriptome sequencing was developed to jointly profile the epigenome in the same single cell and reveal mechanistic insights into the gene regulatory programs that maintain and reset stem cell fate during differentiation.
Journal ArticleDOI
Neuronal filopodia: From stochastic dynamics to robustness of brain morphogenesis.
TL;DR: In this paper , the authors discuss recent advances in understanding of how filopodial dynamics contribute to neuronal differentiation, migration, axonal and dendritic growth and synapse formation.
Journal ArticleDOI
Wnt- and glutamate-receptors orchestrate stem cell dynamics and asymmetric cell division.
Sergi Junyent,Joshua Reeves,James L. A. Szczerkowski,Clare L. Garcin,Tung-Jui Trieu,Matthew Wilson,Jethro Lundie-Brown,Shukry J. Habib +7 more
TL;DR: In this paper, the Wnt-pathway is part of a signalling network that regulates many aspects of cell biology, including early Wnt3a-recruitment, dynamics on the cell membrane, and orientation of the spindle towards a Wnt 3a-source at mitosis.
Journal ArticleDOI
Wnt signalling in cell division: from mechanisms to tissue engineering.
TL;DR: In this article , the authors discuss the bidirectional relationship between Wnt activity and mitosis and demonstrate how various Wnt-dependent mechanisms control spindle dynamics, chromosome segregation, and asymmetric cell division.
Posted ContentDOI
Wnt- and Glutamate-receptors orchestrate stem cell dynamics and asymmetric cell division
Sergi Junyent,Joshua Reeves,James L. A. Szczerkowski,Clare L. Garcin,Tung-Jui Trieu,Matthew Wilson,Shukry J. Habib +6 more
TL;DR: The early Wnt ligand-membrane interaction is scrutinized, linking roles of Wnt-pathway components and crosstalk with iGluRs in guiding cell fate determination by oriented ACD.
References
More filters
Journal ArticleDOI
Epithelial–mesenchymal transitions in tumour progression
TL;DR: Epithelial–mesenchymal transition provides a new basis for understanding the progression of carcinoma towards dedifferentiated and more malignant states.
Journal ArticleDOI
Ultrasensitive fluorescent proteins for imaging neuronal activity.
Tsai Wen Chen,Trevor J. Wardill,Trevor J. Wardill,Yi Sun,Stefan R. Pulver,Sabine L. Renninger,Amy Baohan,Amy Baohan,Eric R. Schreiter,Rex Kerr,Michael B. Orger,Vivek Jayaraman,Loren L. Looger,Karel Svoboda,Douglas S. Kim +14 more
TL;DR: A family of ultrasensitive protein calcium sensors (GCaMP6) that outperformed other sensors in cultured neurons and in zebrafish, flies and mice in vivo are developed and provide new windows into the organization and dynamics of neural circuits over multiple spatial and temporal scales.
Journal ArticleDOI
Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche.
Toshiro Sato,Robert G.J. Vries,Hugo J. Snippert,Marc van de Wetering,Nick Barker,Daniel E. Stange,Johan H. van Es,Arie Abo,Pekka Kujala,Peter J. Peters,Hans Clevers +10 more
TL;DR: It is concluded that intestinal cryptvillus units are self-organizing structures, which can be built from a single stem cell in the absence of a non-epithelial cellular niche.
Journal ArticleDOI
The ground state of embryonic stem cell self-renewal
Qi-Long Ying,Jason Wray,Jennifer Nichols,Laura Batlle-Morera,Bradley W. Doble,James R. Woodgett,Philip Cohen,Austin Smith +7 more
TL;DR: It is shown that extrinsic stimuli are dispensable for the derivation, propagation and pluripotency of ES cells and reveal that ES cells have an innate programme for self-replication that does not require extrinsics instruction.
Journal ArticleDOI
Wnt proteins are lipid-modified and can act as stem cell growth factors
Karl Willert,Jeffrey Brown,Esther Danenberg,Andrew W. Duncan,Irving L. Weissman,Tannishtha Reya,John R. Yates,Roel Nusse +7 more
TL;DR: This work isolated active Wnt molecules, including the product of the mouse Wnt3a gene, and found the proteins to be palmitoylated on a conserved cysteine, indicating that the lipid is important for signalling.
Related Papers (5)
Role of cell-cell adhesion complexes in embryonic stem cell biology.
Tim Pieters,Frans van Roy +1 more