Journal ArticleDOI
Polyprotein gene expression: generation of diversity of neuroendocrine peptides.
TLDR
The present review focuses on the structure of the polyprotein Gene Structures of the Precursor Polypeptide and the approaches used in terms of Peptide approach, strategy, and values.Abstract:
PERSPECTIVES AND SUMMARy 666 SCOPE OF THE REVIEW 668 CHARACTERISTICS OF THE PRECURSORS 669 Sites of Synthesis of Precursors 672 APPROACHES AND STRATEGIES USED IN CHARACTERIZING THE PRECURSOR PROTEINS....... 673 The Peptide Approach 674 The Nucleic Acid Approach 675 STRUCTURE OF POLYPROTEIN GENES 679 Isolation of Genomic Clones 679 Polyprotein Genes in Which Exons Do Not Represent Individual Functional Domains of the Precursor M olecule 680 Polyprotein Genes in Which Exonic Regions Represent Individual Bioactive Domains of the Precursor Polypeptide 682 Nonmammalian Polyprotein Gene Structures 684read more
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The human endothelin family: three structurally and pharmacologically distinct isopeptides predicted by three separate genes.
Akihiro Inoue,Masashi Yanagisawa,Sadao Kimura,Yoshitoshi Kasuya,Takashi Miyauchi,Katsutoshi Goto,Tomoh Masaki +6 more
TL;DR: Three distinct human endothelin-related genes were cloned by screening a genomic DNA library under a low hybridization stringency with a synthetic oligonucleotide probe encoding a portion of the endotheli sequence, suggesting the possible existence of endothelins receptor subtypes.
Journal ArticleDOI
Magainins, a class of antimicrobial peptides from Xenopus skin: isolation, characterization of two active forms, and partial cDNA sequence of a precursor
TL;DR: A family of peptides with broad-spectrum antimicrobial activity has been isolated from the skin of the African clawed frog Xenopus laevis and appears to represent a previously unrecognized class of vertebrate antimicrobial activities.
Journal ArticleDOI
A parathyroid hormone-related protein implicated in malignant hypercalcemia: cloning and expression
Larry J. Suva,G. A. Winslow,Richard E.H. Wettenhall,R Hammonds,Jane M. Moseley,H. Diefenbach-Jagger,Christine Rodda,Bruce E. Kemp,H. Rodriguez,E. Y. Chen,P. J. Hudson,T. J. Martin,William I. Wood +12 more
TL;DR: The isolation of full-length complementary DNA clones of a putative hypercalcemia factor, and the expression from the cloned DNA of the active protein in mammalian cells that has significant homology with parathyroid hormone in the amino-terminal region are reported.
Journal ArticleDOI
Analysis of the aphthovirus 2A/2B polyprotein 'cleavage' mechanism indicates not a proteolytic reaction, but a novel translational effect: a putative ribosomal 'skip'.
Michelle L. L. Donnelly,Garry A. Luke,Amit P. Mehrotra,Xuejun Li,Lorraine E. Hughes,David Gani,Martin D. Ryan +6 more
TL;DR: It is proposed that the FMDV 2A sequence, rather than representing a proteolytic element, modifies the activity of the ribosome to promote hydrolysis of the peptidyl(2A)-tRNA(Gly) ester linkage, thereby releasing the polypeptide from the translational complex, in a manner that allows the synthesis of a discrete downstream translation product to proceed.
Journal ArticleDOI
Human furin is a calcium-dependent serine endoprotease that recognizes the sequence Arg-X-X-Arg and efficiently cleaves anthrax toxin protective antigen.
TL;DR: The hypothesis that furin processes protein precursors containing this cleavage site motif in the exocytic pathway is supported and the possibility that the enzyme also cleaves extracellular substrates, including PA, is raised.