Journal ArticleDOI
Production of both interstitial and basement membrane procollagens by fibroblastic WI-38 cells from human embryonic lung.
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TLDR
Locally obtained human embryonic lung cells secreted the same procollagens, but neither embryonic nor adult human skin fibroblasts were found to secrete type IV procollagen in amounts detectable by the same methods.About:
This article is published in Biochemical and Biophysical Research Communications.The article was published on 1980-04-14. It has received 35 citations till now. The article focuses on the topics: Procollagen peptidase & Basement membrane.read more
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Synthesis of type IV collagen and laminin in cultures of skeletal muscle cells and their assembly on the surface of myotubes.
TL;DR: Staining for extracellular laminin was stronger in mass cultures than in myogenic clones, suggesting that secretion and deposition of components of the basal lamina on the myotube surface are complex processes which may involve cooperation between myogenic and fibrogenic cells.
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Extracellular matrix proteins of human epidermal keratinocytes and feeder 3T3 cells.
TL;DR: The production in culture conditions of basal lamina glycoproteins by the fibroblastic feeder cells may promote the attachment and growth of the cocultured keratinocytes.
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Meningeal cells organize the superficial glia limitans of the cerebellum and produce components of both the interstitial matrix and the basement membrane.
TL;DR: Findings indicate that meningeal cells produce molecular components of both the interstitial matrix and the basement membrane, and are involved in the morphological transformation of glial fibres into the endfeet which constitute the superficial glia limitans.
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Acetaldehyde and lactate stimulate collagen synthesis of cultured baboon liver myofibroblasts
Eeva-Riitta Savolainen,Eeva-Riitta Savolainen,Maria Anna Leo,Maria Anna Leo,Rupert Timpl,Rupert Timpl,Charles S. Lieber,Charles S. Lieber +7 more
TL;DR: To study the effects of ethanol (and its oxidation product acetaldehyde and associated metabolite lactate) on myofibroblast collagen synthesis, the cell cultures were incubated for 24 h in a medium containing either 50 mM ethanol, 200 μM acetaldehyde, or 5 mM lactate.
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Morphology of the basement membrane.
TL;DR: The main interest is, of course, focused on the description of the BM‐structure up to the macromolecular level and the special forms of this structure and the relationship between morphology and biochemistry.
References
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Journal ArticleDOI
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
Journal Article
Cleavage of structural proteins during the assemble of the head of bacterio-phage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products as mentioned in this paper.
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A Film Detection Method for Tritium‐Labelled Proteins and Nucleic Acids in Polyacrylamide Gels
TL;DR: A simple method for detecting 3H in polyacrylamide gels by scintillation autography (fluorography) using X-ray film, which is ten times more sensitive than conventional autoradiography of isotopes with higher emission energies.
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The limited in vitro lifetime of human diploid cell strains
TL;DR: The survival curves obtained with human diploid cell strains are comparable to “multiple-hit” or “ multiple-target” curves obtain with other biological systems where an initial threshold dose is required before an exponential form of the curve is established.
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Purification of fibronectin from human plasma by affinity chromatography under non-denaturing conditions.
TL;DR: It is proposed that the method would be useful in preparation of fibronectin for studies on its biological activities, where it is important that the protein is obtained in a native state.