Journal ArticleDOI
Proteome analysis of rice tissues by two-dimensional electrophoresis: an approach to the investigation of gibberellin regulated proteins.
Reads0
Chats0
TLDR
The results indicate that the proteome databases assembled in these studies will be useful for the rapid assessment of changes in protein content in specific tissues, and that proteins regulated by GA may play a significant role in tissue growth.Abstract:
Protein databases constructed using high-resolution two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) were used to explore the proteome expressed in various rice tissues. Proteins from leaf sheath, root, and cultured suspension cells were systematically analyzed using 2D-PAGE, mass spectrometry and Edman sequencing, followed by database searching. In all, 79 of the 431 spots detected by 2D-PAGE in the leaf sheath, 73 of the 508 spots in the root and 140 of the 962 spots in the cultured suspension cells could be identified. Protein lists were constructed for each tissue and used to investigate the effects of gibberellin (GA) treatment. In the leaf sheath, root and cultured suspension cells, 8, 21, and 14 of the identified proteins, respectively, were regulated by GA. These proteins included polypeptides involved in general metabolism, energy production, transcriptional regulation and signal transduction in the leaf sheath; in metabolism and defense in the root; and in metabolism, energy production, cell growth, defense and signal transduction in the cultured suspension cells. These results indicate that the proteome databases assembled in these studies will be useful for the rapid assessment of changes in protein content in specific tissues, and that proteins regulated by GA may play a significant role in tissue growth.read more
Citations
More filters
Journal ArticleDOI
Highly Sensitive and High-Throughput Analysis of Plant Hormones Using MS-Probe Modification and Liquid Chromatography–Tandem Mass Spectrometry: An Application for Hormone Profiling in Oryza sativa
Mikiko Kojima,Tomoe Kamada-Nobusada,Hirokazu Komatsu,Kentaro Takei,Takeshi Kuroha,Masaharu Mizutani,Motoyuki Ashikari,Miyako Ueguchi-Tanaka,Makoto Matsuoka,Koji Suzuki,Hitoshi Sakakibara +10 more
TL;DR: Application of this method to plant hormone profiling enabled us to draw organ distribution maps of hormone species in rice and also to identify interactions among the four major hormones in the rice gibberellin signaling mutants.
Journal ArticleDOI
Proteomic analysis of rice seedlings during cold stress
Makoto Hashimoto,Setsuko Komatsu +1 more
TL;DR: It was concluded that proteins related to energy metabolism were up‐regulated, and defense‐related proteins were down‐regulated in leaf blades, by cold stress, suggesting that energy production is activated in the chilling environment.
Journal ArticleDOI
Gibberellins and DELLAs: central nodes in growth regulatory networks
TL;DR: The role of GAs as central nodes in networks connecting environmental inputs to growth are discussed, with dynamic and rapid regulation that mostly occurs at the protein level, directly affecting the activity and transcription of effectors.
Journal ArticleDOI
Crop proteomics: Aim at sustainable agriculture of tomorrow
TL;DR: The recent progress in crop proteomics is described and the achievements made in understanding the proteomes of major crops are highlighted, with a major emphasis on crop responses to abiotic stresses.
Journal ArticleDOI
Rice proteomics: A cornerstone for cereal food crop proteomes
TL;DR: The history and the current state of monocot proteomes, including barley, maize, and wheat are looked at, with a central focus on rice, which has the most extensive proteomic coverage to date.
References
More filters
Journal ArticleDOI
A revised medium for rapid growth and bio assays with tobacco tissue cultures
Toshio Murashige,Folke Skoog +1 more
TL;DR: In vivo redox biosensing resolves the spatiotemporal dynamics of compartmental responses to local ROS generation and provide a basis for understanding how compartment-specific redox dynamics may operate in retrograde signaling and stress 67 acclimation in plants.
Journal ArticleDOI
High resolution two-dimensional electrophoresis of proteins.
TL;DR: This technique provides a method for estimation of the number of proteins made by any biological system and can resolve proteins differing in a single charge and consequently can be used in the analysis of in vivo modifications resulting in a change in charge.
Journal ArticleDOI
Signal transduction by receptors with tyrosine kinase activity
Axel Ullrich,Joseph Schlessinger +1 more
TL;DR: Cet article synthese montre comment des recepteurs membranaires a activite tyrosine kinase peuvent etre impliques dans la transduction and notamment jouent le role de signal de the transduction.
Journal ArticleDOI
Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis.
TL;DR: A rapid and convenient method for peptide mapping of proteins has been developed that involves partial enzymatic proteolysis in the presence of sodium dodecyl sulfate and analysis of the cleavage products by polyacrylamide gel electrophoresis.
Related Papers (5)
A draft sequence of the rice genome (Oryza sativa L. ssp indica)
Stephen A. Goff,Darrell O. Ricke,Tien-Hung Lan,Gernot G. Presting,Ronglin Wang,Molly Dunn,Jane Glazebrook,Allen Sessions,Paul Oeller,Hemant Varma,David Hadley,Don Hutchison,Christopher M. Martin,Fumiaki Katagiri,B. Markus Lange,Todd Moughamer,Yu Xia,Paul Budworth,Jingping Zhong,Trini Miguel,Uta Paszkowski,Shiping Zhang,Michelle Colbert,Wei-lin Sun,Lili Chen,Bret Cooper,Sylvia Park,Todd Charles Wood,Long Mao,Peter H. Quail,Rod A. Wing,Ralph A. Dean,Yeisoo Yu,Andrey Zharkikh,Richard Shen,Sudhir Sahasrabudhe,Alun Thomas,Rob Cannings,Alexander Gutin,Dmitry Pruss,Julia Reid,Sean V. Tavtigian,J.T. Mitchell,Glenn Eldredge,Terri Scholl,Rose Mary Miller,Satish Bhatnagar,Nils Adey,Todd Rubano,Nadeem Tusneem,Rosann Robinson,Jane Feldhaus,Teresita Macalma,Arnold R. Oliphant,Steven P. Briggs +54 more