Journal ArticleDOI
Proteomic analysis of mature adipocytes from obese patients in relation to aging.
Assim A. Alfadda,Hicham Benabdelkamel,Afshan Masood,Amr S. Moustafa,Reem M. Sallam,Abdulelah F. Bassas,Mark W. Duncan,Mark W. Duncan +7 more
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TLDR
Proteomic analysis of subcutaneous adipose tissue reveals differences in the abundance of proteins in adipocytes isolated from young vs. old individuals, involved in the regulation of apoptosis, cellular senescence and inflammatory response, which are common pathologic events in both obesity and aging.About:
This article is published in Experimental Gerontology.The article was published on 2013-11-01. It has received 61 citations till now. The article focuses on the topics: Adipose tissue & Adipocyte.read more
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Nrf2 signaling and redox homeostasis in the aging heart: A potential target to prevent cardiovascular diseases?
TL;DR: Interventions that aim to reduce oxidative stress by supplementing with exogenous antioxidants by activating the nuclear factor related-2 factor (Nrf2) pathway; one of the best studied molecules in cellular redox homeostasis and a master regulator of the antioxidant and phase II detoxification response.
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Proteome-wide alterations on adipose tissue from obese patients as age-, diabetes- and gender-specific hallmarks.
María Gómez-Serrano,Emilio Camafeita,Eva García-Santos,Juan Antonio López,Miguel A. Rubio,Andrés Sánchez-Pernaute,Antoni Torres,Jesús Vázquez,Belén Peral,Belén Peral,Belén Peral +10 more
TL;DR: In diabetic patients, remarkable gender-specific hallmarks were discovered regarding redox status, immune response and adipose tissue accumulation, and systems biology analysis highlighted mitochondrial proteins that could play a key role in the age-dependent pathophysiology of T2DM.
Journal ArticleDOI
A proteomic approach to obesity and type 2 diabetes.
Elena López-Villar,Gabriel Á. Martos-Moreno,Gabriel Á. Martos-Moreno,Julie A. Chowen,Julie A. Chowen,Shigeru Okada,Shigeru Okada,John J. Kopchick,John J. Kopchick,Jesús Argente,Jesús Argente +10 more
TL;DR: By applying the current improvements in proteomic methodologies and new strategies that could be employed, novel therapeutic and/or diagnostic protein targets will be discovered in the obesity/Type 2 diabetes area.
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Increased macromolecular damage due to oxidative stress in the neocortex and hippocampus of WNIN/Ob, a novel rat model of premature aging
TL;DR: It can be concluded that increased oxidative stress-induced damage of macromolecules, probably due to reduced activity of antioxidant enzymes, is associated with premature aging in WNIN/Ob obese rats.
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Differences in the Plasma Proteome of Patients with Hypothyroidism before and after Thyroid Hormone Replacement: A Proteomic Analysis
TL;DR: The comparison of the plasma proteome between the hypothyroid vs euthyroid states revealed differences in the abundance of proteins involved in regulating the acute phase response.
References
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Journal ArticleDOI
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
Journal Article
Cleavage of structural proteins during the assemble of the head of bacterio-phage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products as mentioned in this paper.
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Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
TL;DR: A method has been devised for the electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets that results in quantitative transfer of ribosomal proteins from gels containing urea.
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Mass Spectrometric Sequencing of Proteins from Silver-Stained Polyacrylamide Gels
TL;DR: Silver staining allows a substantial shortening of sample preparation time and may, therefore, be preferable over Coomassie staining, and this work removes a major obstacle to the low-level sequence analysis of proteins separated on polyacrylamide gels.
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Adipocyte death defines macrophage localization and function in adipose tissue of obese mice and humans
Saverio Cinti,Grant A. Mitchell,Giorgio Barbatelli,Incoronata Murano,Enzo Ceresi,Emanuela Faloia,Shupei Wang,Mélanie Fortier,Andrew S. Greenberg,Martin S. Obin +9 more
TL;DR: It is demonstrated that >90% of all macrophages in WAT of obese mice and humans are localized to dead adipocytes, where they fuse to form syncytia that sequester and scavenge the residual “free” adipocyte lipid droplet and ultimately form multinucleate giant cells, a hallmark of chronic inflammation.