Journal ArticleDOI
Purification and properties of phosphatidylinositol-specific phospholipase C of Bacillus thuringiensis
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TLDR
A phosphatidylinositol-specific phospholipase C was purified from the culture broth of Bacillus thuringiensis to a homogeneous state as indicated by polyacrylamide gel electrophoresis and was maximally active at pH 7.5 and not influenced by EDTA.About:
This article is published in Biochimica et Biophysica Acta.The article was published on 1980-07-14. It has received 94 citations till now. The article focuses on the topics: Phospholipase C & Enzyme assay.read more
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The structure, biosynthesis and functions of glycosylphosphatidylinositol anchors, and the contributions of trypanosome research
TL;DR: Apart from providing stable membrane anchorage, GPI anchors have been implicated in the sequestration of GPI-anchored proteins into specialised membrane microdomains, known as lipid rafts, and in signal transduction events.
Journal ArticleDOI
The glycosyl-phosphatidylinositol anchor of membrane proteins.
TL;DR: Etude de la synthese des groupements fonctionnels glycosyl-phosphatidylinositol se liant aux extremites C terminales des proteines procaryotique ou eucaryotiques, permettant leur determination et leur identification.
Journal ArticleDOI
Bacterial phospholipases C.
TL;DR: A greater understanding of the modes of action and structure-function relationships of phospholipases C will facilitate the interpretation of studies in which these enzymes are used as membrane probes and will enhance the use of these proteins as models for eukaryotic phospholIPases C.
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T-cadherin, a novel cadherin cell adhesion molecule in the nervous system lacks the conserved cytoplasmic region.
TL;DR: Biochemical analysis revealed that this truncated (T) cadherin is attached to the neuronal plasma membrane through a glycosyl phosphatidylinositol anchor, consistent with a putative role of T-cadherin in axon growth and guidance.
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Glycosylphosphatidylinositol (GPI)-Anchored Proteins
TL;DR: The carboxyl-terminal signal peptide containing hydrophobic tail is characterized by genetic analysis and shown to be essential for GPI modification of protein.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
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Assay of proteins in the presence of interfering materials.
TL;DR: The Lowry protein assay is a sensitive but highly nonspecific procedure that has been modified so that protein can be assayed in the presence of interfering chemicals.
Journal ArticleDOI
On tyrosine and tryptophane determinations in proteins
Otto Folin,Vintila Ciocalteu +1 more
TL;DR: In this article, the authors summarized research into the existing methods for the quantitative determination of tyrosine and tryptophane in proteins, including the Folin-Looney method, which is based on reaction of a phosphotungstic phosphomolybdic acid in a phenol solution.
Journal ArticleDOI
Crystalline soybean trypsin inhibitor : ii. general properties.
TL;DR: Crystalline soy protein when denatured is readily digestible by pepsin, and less readily by chymotrypsin and by trypsin, which results in a proportional gain in the inhibiting activity.
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