QTL-seq: rapid mapping of quantitative trait loci in rice by whole genome resequencing of DNA from two bulked populations.
Hiroki Takagi,Akira Abe,Kentaro Yoshida,Shunichi Kosugi,Satoshi Natsume,Chikako Mitsuoka,Aiko Uemura,Hiroe Utsushi,Muluneh Tamiru,Shohei Takuno,Hideki Innan,Liliana M. Cano,Sophien Kamoun,Ryohei Terauchi +13 more
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TLDR
Simulation study showed that QTL-seq is able to detect QTLs over wide ranges of experimental variables, and the method can be generally applied in population genomics studies to rapidly identify genomic regions that underwent artificial or natural selective sweeps.Abstract:
SUMMARY The majority of agronomically important crop traits are quantitative, meaning that they are controlled by multiple genes each with a small effect (quantitative trait loci, QTLs). Mapping and isolation of QTLs is important for efficient crop breeding by marker-assisted selection (MAS) and for a better understanding of the molecular mechanisms underlying the traits. However, since it requires the development and selection of DNA markers for linkage analysis, QTL analysis has been time-consuming and labor-intensive. Here we report the rapid identification of plant QTLs by whole-genome resequencing of DNAs from two populations each composed of 20–50 individuals showing extreme opposite trait values for a given phenotype in a segregating progeny. We propose to name this approach QTL-seq as applied to plant species. We applied QTL-seq to rice recombinant inbred lines and F2 populations and successfully identified QTLs for important agronomic traits, such as partial resistance to the fungal rice blast disease and seedling vigor. Simulation study showed that QTL-seq is able to detect QTLs over wide ranges of experimental variables, and the method can be generally applied in population genomics studies to rapidly identify genomic regions that underwent artificial or natural selective sweeps.read more
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References
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Journal Article
Human biochemical genetics
TL;DR: For the next few weeks the course is going to be exploring a field that’s actually older than classical population genetics, although the approach it’ll be taking to it involves the use of population genetic machinery.
Journal ArticleDOI
Food Security: The Challenge of Feeding 9 Billion People
H Charles J Godfray,John Beddington,I. R. Crute,Lawrence Haddad,David Lawrence,James F. Muir,Jules Pretty,Sherman Robinson,Sandy M Thomas,Camilla Toulmin +9 more
TL;DR: A multifaceted and linked global strategy is needed to ensure sustainable and equitable food security, different components of which are explored here.