Journal ArticleDOI
Rapid, transient expression of fluorescent fusion proteins in tobacco plants and generation of stably transformed plants.
TLDR
The protocol presented here has underpinned much of the in vivo results highlighting the dynamic nature of the plant secretory pathway and is a relatively fast technique to assess expression of genes of interest.Abstract:
Expression and tracking of fluorescent fusion proteins has revolutionized our understanding of basic concepts in cell biology. The protocol presented here has underpinned much of the in vivo results highlighting the dynamic nature of the plant secretory pathway. Transient transformation of tobacco leaf epidermal cells is a relatively fast technique to assess expression of genes of interest. These cells can be used to generate stable plant lines using a more time-consuming, cell culture technique. Transient expression takes from 2 to 4 days whereas stable lines are generated after approximately 2 to 4 months.read more
Citations
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Redox-sensitive GFP in Arabidopsis thaliana is a quantitative biosensor for the redox potential of the cellular glutathione redox buffer.
Andreas J. Meyer,Thorsten Brach,Laurent Marty,Susanne Kreye,Nicolas Rouhier,Jean-Pierre Jacquot,Rüdiger Hell +6 more
TL;DR: The results with roGFP2 as an artificial GRX target further suggest that redox-triggered changes of biologic processes might be linked directly to the glutathione redox potential via GRX as the mediator.
Journal ArticleDOI
Regulation of Floral Patterning by Flowering Time Genes
TL;DR: The finding of coordinated regulation of SEP3 by flowering time genes reveals a hitherto unknown genetic pathway that prevents premature differentiation of floral meristems and determines the appropriate timing of floral organ patterning.
Journal ArticleDOI
Arabidopsis homolog of the yeast TREX‐2 mRNA export complex: components and anchoring nucleoporin
Qing Lu,Xurong Tang,Xurong Tang,Gang Tian,Gang Tian,Fang Wang,Kede Liu,Vi Nguyen,Susanne E. Kohalmi,Wilfred A. Keller,Edward W. T. Tsang,John J. Harada,Steven J. Rothstein,Yuhai Cui +13 more
TL;DR: In this paper, the putative Arabidopsis thaliana TREX-2 complex and its anchoring nucleoporin were identified and characterized, which is essential for mRNA export in eukaryotes.
Journal ArticleDOI
Control of grain size and rice yield by GL2-mediated brassinosteroid responses.
Ronghui Che,Hongning Tong,Bihong Shi,Yuqin Liu,Shanru Fang,Dapu Liu,Yunhua Xiao,Bin Hu,Linchuan Liu,Hongru Wang,Mingfu Zhao,Chengcai Chu +11 more
TL;DR: The feasibility of modulating specific brassinosteroid responses to improve plant productivity is demonstrated, and a grain-length-associated QTL, GL2, has the potential to improve grain weight and grain yield.
Journal ArticleDOI
Confocal imaging of glutathione redox potential in living plant cells
Markus Schwarzländer,Mark D. Fricker,Christopher Müller,Laurent Marty,Thorsten Brach,J. Novak,Lee J. Sweetlove,Rüdiger Hell,Andreas J. Meyer +8 more
TL;DR: Specific interaction of both roGFPs with glutaredoxin in vitro strongly suggests that in situ both variants preferentially act as sensors for the glutathione redox potential, suggesting that roG FP2 is a reliable probe for dynamic redox imaging in planta.
References
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Journal ArticleDOI
Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana
Steven J. Clough,Andrew F. Bent +1 more
TL;DR: The modified method should facilitate high-throughput transformation of Arabidopsis for efforts such as T-DNA gene tagging, positional cloning, or attempts at targeted gene replacement.
Journal ArticleDOI
Gateway-compatible vectors for plant functional genomics and proteomics.
Keith Earley,Jeremy R. Haag,Olga Pontes,Kristen E. Opper,Tom Juehne,Keming Song,Craig S. Pikaard +6 more
TL;DR: The utility of pEarleyGate destination vectors for the expression of epitope-tagged proteins that can be affinity captured or localized by immunofluorescence microscopy is demonstrated.
Journal ArticleDOI
Agrobacterium-mediated transformation of Arabidopsis thaliana using the floral dip method
TL;DR: The protocol routinely used in the laboratory for the floral dip method for Arabidopsis transformation is described, which can be routinely obtained and a minimum of several hundred independent transgenic lines generated from just two pots of infiltrated plants within 2–3 months.
Journal ArticleDOI
Visualization of protein interactions in living plant cells using bimolecular fluorescence complementation.
Michael Walter,Christina Chaban,Katia Schütze,Oliver Batistič,Katrin Weckermann,Christian Näke,Dragica Blazevic,Christopher Grefen,Karin Schumacher,Claudia Oecking,Klaus Harter,Jörg Kudla +11 more
TL;DR: The implementation of a bimolecular fluorescence complementation technique for visualization of protein-protein interactions in plant cells revealed a remarkable signal fluorescence intensity of interacting protein complexes as well as a high reproducibility and technical simplicity of the method in different plant systems.
Journal ArticleDOI
Agrobacterium tumefaciens-mediated transformation of Arabidopsis thaliana root explants by using kanamycin selection.
TL;DR: A transformation procedure for Arabidopsis root explants based on kanamycin selection was established and an Agrobacterium tumor-inducing Ti plasmid carrying a chimeric neomycin phosphotransferase II gene (neo) was introduced, resulting in transformed seed-producing plants obtained with an efficiency between 20% and 80% within 3 months after gene transfer.
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