Regulation of flagellar motility during biofilm formation
TLDR
The regulation of motility during biofilm formation in Bacillus, Pseudomonas, Vibrio, and Escherichia is reviewed, and it is concluded that the motility-to-biofilm transition, if necessary, likely involves two steps.Abstract:
Many bacteria swim in liquid or swarm over solid surfaces by synthesizing rotary flagella The same bacteria that are motile also commonly form nonmotile multicellular aggregates called biofilms Biofilms are an important part of the lifestyle of pathogenic bacteria, and it is assumed that there is a motility-to-biofilm transition wherein the inhibition of motility promotes biofilm formation The transition is largely inferred from regulatory mutants that reveal the opposite regulation of the two phenotypes Here, we review the regulation of motility during biofilm formation in Bacillus, Pseudomonas, Vibrio, and Escherichia, and we conclude that the motility-to-biofilm transition, if necessary, likely involves two steps In the short term, flagella are functionally regulated to either inhibit rotation or modulate the basal flagellar reversal frequency Over the long term, flagellar gene transcription is inhibited and in the absence of de novo synthesis, flagella are diluted to extinction through growth Both short-term and long-term motility inhibition is likely important to stabilize cell aggregates and optimize resource investment We emphasize the newly discovered flagellar functional regulators and speculate that others await discovery in the context of biofilm formationread more
Citations
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Beyond Risk: Bacterial Biofilms and Their Regulating Approaches.
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TL;DR: The events involved in bacterial biofilm formation are described, the negative and positive aspects associated with bacterial biofilms are listed, the main strategies currently used to regulate establishment of harmful bacterial bioFilms are elaborated as well as certain strategies employed to encourage formation of beneficial bacterialBiofilms.
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Type III secretion systems: the bacterial flagellum and the injectisome
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References
More filters
Journal ArticleDOI
Distinct Sensory Pathways in Vibrio cholerae El Tor and Classical Biotypes Modulate Cyclic Dimeric GMP Levels To Control Biofilm Formation
TL;DR: It is demonstrated that different pandemic strains of V. cholerae modulate c-di-GMP levels and control biofilm formation in response to distinct sensory pathways, and that restoring HapR in V. Cholerae(Cl) reestablishes QS-dependent repression of exopolysaccharide production.
Journal ArticleDOI
Acetyl phosphate-sensitive regulation of flagellar biogenesis and capsular biosynthesis depends on the Rcs phosphorelay.
TL;DR: Results show that both acetyl∼P‐sensitive activation of capsular biosynthesis and acetyl ∼P‐ sensitive repression of flagellar biogenesis require the Rcs phosphorelay, and provide strong genetic support for the hypothesis that RcsC can function as either a kinase or a phosphatase dependent on environmental conditions.
Journal ArticleDOI
The EpsE Flagellar Clutch Is Bifunctional and Synergizes with EPS Biosynthesis to Promote Bacillus subtilis Biofilm Formation
TL;DR: The transition from motility to biofilm formation may be governed by a single bifunctional enzyme, and both functions of EpsE synergize to stabilize cell aggregates and relieve selective pressure to abolish motility by genetic mutation.
Journal ArticleDOI
c-di-GMP turn-over in Clostridium difficile is controlled by a plethora of diguanylate cyclases and phosphodiesterases.
TL;DR: The results indicate that, in contrast to most Gram-positive bacteria including its closest relatives, C. difficile encodes a large assortment of functional DGCs and PDEs, revealing that c-di-GMP signalling is an important and well-conserved signal transduction system in this human pathogen.
Journal ArticleDOI
SlrR/SlrA controls the initiation of biofilm formation in Bacillus subtilis.
TL;DR: It is shown that mutations in late‐flagellar genes prevent pellicle formation at an initiation step in Bacillus subtilis strain ATCC 6051, which has a frameshift mutation in the ywcC gene, which encodes a TetR‐type transcriptional repressor.
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