Journal ArticleDOI
Requirement for the yeast gene LON in intramitochondrial proteolysis and maintenance of respiration
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TLDR
The role of protein degradation in mitochondrial homeostasis was explored by cloning of a gene from Saccharomyces cerevisiae that encodes a protein resembling the adenosine triphosphate (ATP)-dependent bacterial protease Lon, which has a typical mitochondrial matrix-targeting sequence at its amino terminus.Abstract:
The role of protein degradation in mitochondrial homeostasis was explored by cloning of a gene from Saccharomyces cerevisiae that encodes a protein resembling the adenosine triphosphate (ATP)-dependent bacterial protease Lon. The predicted yeast protein has a typical mitochondrial matrix-targeting sequence at its amino terminus. Yeast cells lacking a functional LON gene contained a nonfunctional mitochondrial genome, were respiratory-deficient, and lacked an ATP-dependent proteolytic activity present in the mitochondria of Lon+ cells. Lon- cells were also impaired in their ability to catalyze the energy-dependent degradation of several mitochondrial matrix proteins and they accumulated electron-dense inclusions in their mitochondrial matrix.read more
Citations
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Journal ArticleDOI
Ionizing radiation-induced metabolic oxidative stress and prolonged cell injury
TL;DR: The role of mitochondria in the delayed outcomes of ionization radiation is discussed, and different types of radiation vary in their linear energy transfer (LET) properties, and their effects on various aspects of mitochondrial physiology are discussed.
Journal ArticleDOI
Mitochondrial import efficiency of ATFS-1 regulates mitochondrial UPR activation.
Amrita M. Nargund,Mark W. Pellegrino,Christopher J. Fiorese,Christopher J. Fiorese,Brooke M. Baker,Cole M. Haynes,Cole M. Haynes +6 more
TL;DR: The mechanism by which ATFS-1 (activating transcription factor associated with stress–1) senses mitochondrial stress and communicates with the nucleus during the mitochondrial unfolded protein response (UPRmt) in Caenorhabditis elegans is examined and it is found that the key point of regulation is the mitochondrial import efficiency of ATfs-1.
Journal ArticleDOI
Proteases and their targets in Escherichia coli.
TL;DR: Many of the specific proteases found in E. coli are well-conserved in both prokaryotes and eukaryotes, and serve critical functions in developmental systems.
Journal ArticleDOI
Protein quality control: triage by chaperones and proteases.
TL;DR: A general model for what can be thought of as a triage system for handling misfolded proteins in vivo is proposed, assuring swift re folding of proteins with functional potential and rapid degradation of irreversibly denatured or damaged pro teins.
Journal ArticleDOI
Lon protease preferentially degrades oxidized mitochondrial aconitase by an ATP-stimulated mechanism.
TL;DR: Lon protease, an ATP-stimulated mitochondrial matrix protein, selectively recognizes and degrades the oxidized, hydrophobic form of aconitase after mild oxidative modification, but that severe oxidation results in ac onitase aggregation, which makes it a poor substrate for Lon.
References
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Journal ArticleDOI
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