Retrospective analysis of outcomes after IVF using an aneuploidy risk model derived from time-lapse imaging without PGS
Alison Campbell,Simon Fishel,Natalie Bowman,Samantha Duffy,Mark Sedler,Simon Thornton. Alison Campbell,Simon Thornton +6 more
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TLDR
The clinical relevance of the aneuploidy risk classification model is demonstrated and a novel, non-invasive method of embryo selection to yield higher implantation and live birth rates without PGS is introduced.Abstract:
Time-lapse imaging of human preimplantation IVF embryos has enabled objective algorithms based on novel observations of development (morphokinetics) to be used for clinical selection of embryos. Embryo aneuploidy, a major cause of IVF failure, has been correlated with specific morphokinetic variables used previously to develop an aneuploidy risk classification model. The purpose of this study was to evaluate the effectiveness and potential impact of this model for unselected IVF patients without biopsy and preimplantation genetic screening (PGS). Embryo outcomes - no implantation, fetal heart beat (FHB) and live birth (LB) - of 88 transferred blastocysts were compared according to calculated aneuploidy risk classes (low, medium, high). A significant difference was seen for FHB (P<0.0001) and LB (P<0.01) rates between embryos classified as low and medium risk. Within the low-risk class, relative increases of 74% and 56%, compared with rates for all blastocysts, were observed for FHB and LB respectively. The area under the receiver operating characteristic curve was 0.75 for FHB and 0.74 for LB. This study demonstrates the clinical relevance of the aneuploidy risk classification model and introduces a novel, non-invasive method of embryo selection to yield higher implantation and live birth rates without PGS.read more
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Correlation between aneuploidy, standard morphology evaluation and morphokinetic development in 1730 biopsied blastocysts: a consecutive case series study
Maria Giulia Minasi,Alessandro Colasante,Teresa Riccio,Alessandra Ruberti,Valentina Casciani,Filomena Scarselli,Francesca Spinella,Francesco Fiorentino,Maria Teresa Varricchio,Ermanno Greco +9 more
TL;DR: Correlations were observed, in that euploid human blastocysts showed a higher percentage with top quality inner cell mass (ICM) and trophectoderm (TE), higher expansion grades and shorter time to start of blastulation, expansion and hatching, compared to aneuploid ones.
Journal ArticleDOI
Proposed guidelines on the nomenclature and annotation of dynamic human embryo monitoring by a time-lapse user group
H. Nadir Ciray,Alison Campbell,Inge Agerholm,J. Aguilar,Sandrine Chamayou,Marga Esbert,Shabana Sayed +6 more
TL;DR: The adoption of the proposed guidelines for defining annotation practice and universal nomenclature would help unify time-lapse monitoring practice, allow validation of published embryo selection algorithms and facilitate progress in this field.
Journal ArticleDOI
Development of a generally applicable morphokinetic algorithm capable of predicting the implantation potential of embryos transferred on Day 3
TL;DR: The algorithm presented here can be used independently of culture conditions and fertilization method and provides predictive power not surpassed by other published algorithms for ranking embryos according to their blastocyst formation potential.
Journal ArticleDOI
Increasing the probability of selecting chromosomally normal embryos by time-lapse morphokinetics analysis.
N. Basile,M. Nogales,F. Bronet,M. Florensa,Marissa Riqueiros,Lorena Rodrigo,Juan A. Garcia-Velasco,Marcos Meseguer +7 more
TL;DR: Ch Chromosomally normal and abnormal embryos have different kinetic behavior and the proposed algorithm serves as a tool to classify embryos and to increase the probability of noninvasively selecting normal embryos.
Journal ArticleDOI
Morphokinetic analysis and embryonic prediction for blastocyst formation through an integrated time-lapse system.
Y. Motato,María José de los Santos,María José Escribá,Belén Aparicio Ruiz,José Remohí,Marcos Meseguer +5 more
TL;DR: Two multivariable models are proposed based on the findings to classify embryos according to their probabilities of blastocyst stage and implantation in the largest data set ever reported of human blastocysts.
References
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TL;DR: It is expected that the definition of common terminology and standardization of laboratory practice related to embryo morphology assessment will result in more effective comparisons of treatment outcomes.
Journal ArticleDOI
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TL;DR: Evidence suggests that cohesin acts as a novel topological device that traps chromosomal DNA within a large tripartite ring formed by its core subunits.
Journal ArticleDOI
The use of morphokinetics as a predictor of embryo implantation
TL;DR: A multivariable model is proposed based on the findings to classify embryos according to their probability of implantation and it is proposed that the image acquisition and time-lapse analysis system makes it possible to determine exact timing of embryo cleavages in a clinical setting.
Journal ArticleDOI
Selection of single blastocysts for fresh transfer via standard morphology assessment alone and with array CGH for good prognosis IVF patients: results from a randomized pilot study.
Zhihong Yang,Jiaen Liu,Gary S. Collins,Shala A Salem,Xiaohong Liu,Sarah S Lyle,Alison C Peck,E Scott Sills,Rifaat D Salem +8 more
TL;DR: This is the first description of aCGH fully integrated with a clinical IVF program to select single blastocysts for fresh SET in good prognosis patients and highlights the inherent imprecision of SET when conventional morphology is used alone.