Journal ArticleDOI
RNA decay machines: deadenylation by the Ccr4-not and Pan2-Pan3 complexes
TLDR
This article discusses specialised and redundant functions of the deadenylases and the importance of Ccr4-Not subunits in the regulation of physiological processes, and comments on generic and specific mechanisms of recruitment of CCr4- not and Pan2-Pan3 to mRNAs.About:
This article is published in Biochimica et Biophysica Acta.The article was published on 2013-06-01. It has received 224 citations till now. The article focuses on the topics: CCR4-NOT complex & Mature messenger RNA.read more
Citations
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Journal ArticleDOI
Towards a molecular understanding of microRNA-mediated gene silencing
Stefanie Jonas,Elisa Izaurralde +1 more
TL;DR: Understanding of the mechanisms of silencing is enhanced, making it possible to describe in molecular terms a continuum of direct interactions from miRNA target recognition to mRNA deadenylation, decapping and 5′-to-3′ degradation.
Journal ArticleDOI
The complexity of miRNA-mediated repression.
Anna Wilczynska,Martin Bushell +1 more
TL;DR: The most recent advances in the understanding of the molecular underpinnings of miRNA-mediated repression are discussed and the multitude of regulatory mechanisms that modulate miRNA function are highlighted.
Journal ArticleDOI
Stress granules, P-bodies and cancer
TL;DR: Evidence implicating RNA granules in the pathogenesis of cancer and their potential as targets for anticancer therapies are examined.
Journal ArticleDOI
The Ccr4‐Not complex is a key regulator of eukaryotic gene expression
TL;DR: Ccr4‐Not is a master regulator of eukaryotic gene expression and is a key player in mRNA decay, generic mRNA decay that follows normal translation termination, co‐translational mRNA decay of transcripts on which the ribosomes stall durably or which carry a non‐sense mutation and finally mRNA Decay that is induced by external signaling for a change in genetic programming.
Journal ArticleDOI
A DDX6-CNOT1 complex and W-binding pockets in CNOT9 reveal direct links between miRNA target recognition and silencing.
Ying Chen,Andreas Boland,Duygu Kuzuoğlu-Öztürk,Praveen Bawankar,Belinda Loh,Chung Te Chang,Oliver Weichenrieder,Elisa Izaurralde +7 more
TL;DR: The structural basis for the repressive activity of CCR4-NOT and its interaction with TNRC6/GW182s is elucidated, and the crystal structure of this complex demonstrates striking similarity to the eIF4G-eIF4A complex.
References
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Journal ArticleDOI
Functional organization of the yeast proteome by systematic analysis of protein complexes
Anne-Claude Gavin,Markus Bösche,Roland Krause,Paola Grandi,Martina Marzioch,Andreas Bauer,Jörg Schultz,Jens Rick,Anne-Marie Michon,Cristina-Maria Cruciat,Marita Remor,Christian Höfert,Malgorzata Schelder,Miro Brajenovic,Heinz Ruffner,Alejandro Merino,Karin Klein,Manuela Hudak,David Dickson,Tatjana Rudi,Volker Gnau,Angela Bauch,Sonja Bastuck,Bettina Huhse,Christina Leutwein,Marie-Anne Heurtier,Richard R. Copley,Angela Edelmann,Erich Querfurth,Vladimir Rybin,Gerard Drewes,Manfred Raida,Tewis Bouwmeester,Peer Bork,Bertrand Séraphin,Bernhard Kuster,Gitte Neubauer,Giulio Superti-Furga +37 more
TL;DR: The analysis provides an outline of the eukaryotic proteome as a network of protein complexes at a level of organization beyond binary interactions, which contains fundamental biological information and offers the context for a more reasoned and informed approach to drug discovery.
Journal ArticleDOI
MicroRNAs direct rapid deadenylation of mRNA.
TL;DR: It is shown that in mammalian cells this reduction in message abundance is a consequence of accelerated deadenylation, which leads to rapid mRNA decay, suggesting that miRNAs use two distinct posttranscriptional mechanisms to down-regulate gene expression.
Journal ArticleDOI
mRNA degradation by miRNAs and GW182 requires both CCR4:NOT deadenylase and DCP1:DCP2 decapping complexes
Isabelle Behm-Ansmant,Jan Rehwinkel,Tobias Doerks,Alexander Stark,Peer Bork,Elisa Izaurralde +5 more
TL;DR: It is shown that depletion of GW182 leads to changes in mRNA expression profiles strikingly similar to those observed in cells depleted of the essential Drosophila miRNA effector AGO1, indicating that GW182 functions in the miRNA pathway.
Journal ArticleDOI
The Many Pathways of RNA Degradation
TL;DR: From the earliest comparisons of RNA production with steady-state levels, it has been clear that cells transcribe more RNA than they accumulate, implying the existence of active RNA degradation systems.
Journal ArticleDOI
The enzymes and control of eukaryotic mRNA turnover.
TL;DR: It is highlighted that there are a variety of enzymes with different specificities, suggesting that individual nucleases act on distinct subpopulations of transcripts within the cell and multiple mechanisms by which mRNA degradation could be regulated.