Journal ArticleDOI
Sarcoma viruses carrying ras oncogenes induce differentiation-associated properties in a neuronal cell line
Makoto Noda,Minoru S.H. Ko,Akihiko Ogura,Ding Gan Liu,Takehiko Amano,Toshiya Takano,Yoji Ikawa +6 more
TLDR
It is found that Ki- and Ha-MSV mimic some, if not all, of the activities of NGF in PC12 cells, and there is evidence that the viral oncogenes, v-Ki-ras and v-Ha-ras, are responsible for this phenomenon.Abstract:
The growth-promoting and/or differentiation-blocking activities of Kirsten (Ki-MSV) or Harvey murine sarcoma virus (Ha-MSV) on various types of cells in vitro are well documented. Here we report an unexpected effect of these viruses on a rat phaeochromocytoma cell line, PC12. PC12 cells, which multiply indefinitely in growth medium, are known to respond to nerve growth factor (NGF) by cessation of cell division and expression of several properties resembling those of differentiated sympathetic neurones. We have found that Ki- and Ha-MSV mimic some, if not all, of the activities of NGF in PC12 cells, and there is evidence that the viral oncogenes, v-Ki-ras and v-Ha-ras, are responsible for this phenomenon. This system may be of value for studying the mechanism of action of the v-ras genes as well as the regulatory mechanism of growth and differentiation in neuronal cells.read more
Citations
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Fibroblast growth factor signaling in Caenorhabditis elegans.
TL;DR: This review will describe FGFR‐mediated signal transduction in C. elegans and focus on how genetic studies in the nematode have contributed to the understanding of how FGFRs orchestrate the assembly of intracellular signaling pathways.
Journal ArticleDOI
Tissue localization of Drosophila melanogaster ras transcripts during development.
Dagan Segal,Ben-Zion Shilo +1 more
TL;DR: The tissue distribution of ras transcripts in D. melanogaster suggests that the ras proteins have multiple roles during development which may be related to both the proliferative and differentiated states of the tissues.
Journal ArticleDOI
Autism and genetics: Clinical approach and association study with two markers of HRAS gene
Josiane Hérault,Elisabeth Petit,Joelle Martineau,A. Perrot,Pascal Lenoir,Catherine Cherpi,Catherine Barthélémy,D. Sauvage,Jacques Mallet,Jean Pierre Müh,Gilbert Lelord +10 more
TL;DR: A larger population, well-characterized clinically and genetically, is studied and reports a positive association between autism and two HRAS markers, the 3' marker used in the initial study and an additional marker in exon 1.
Journal ArticleDOI
Studies on ras proteins. Catalytic properties of normal and activated ras proteins purified in the absence of protein denaturants
TL;DR: Normal and activated Ha-ras proteins were produced in Escherichia coli, and purified to an apparent homogeneity without using any protein denaturants, and the binding of GDP to the protein was greatly stabilized by Mg2+.
Journal ArticleDOI
Activation of the c-fos serum-response element by the activated c-Ha-ras protein in a manner independent of protein kinase C and cAMP-dependent protein kinase
TL;DR: Results indicate that the c-Ha-rasval12 protein activates the serum- response element, but not the cAMP-response element in thec-fos gene enhancer, and that the signal pathway from the c Ha-rasVal12 protein to the cfos serum-response elements is independent of protein kinase C and cAMP/cAMP-dependent protein kinases.
References
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A new and rapid colorimetric determination of acetylcholinesterase activity.
TL;DR: A photometric method for determining acetylcholinesterase activity of tissue extracts, homogenates, cell suspensions, etc., has been described and Kinetic constants determined by this system for erythrocyte eholinesterases are presented.
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Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor.
TL;DR: A single cell clonal line which responds reversibly to nerve growth factor (NGF) has been established from a transplantable rat adrenal pheochromocytoma and should be a useful model system for neurobiological and neurochemical studies.
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In yeast, RAS proteins are controlling elements of adenylate cyclase
T. Toda,Isao Uno,Tatsuo Ishikawa,Scott Powers,Tohru Kataoka,Daniel Broek,Scott J. Cameron,James R. Broach,Kunihiro Matsumoto,Michael Wigler +9 more
TL;DR: S. cerevisiae strains containing RAS2val19, a Ras2 gene with a missense mutation analogous to one that activates the transforming potential of mammalian ras genes, have growth and biochemical properties strikingly similar to yeast strains carrying IAC or bcy1.
Journal ArticleDOI
Nerve growth factor-induced increase in electrical excitability and acetylcholine sensitivity of a rat pheochromocytoma cell line
TL;DR: A cloned line of rat pheochromocytoma cells (PC12) which responds to nerve growth factor by cessation of division and extension of neurone-like processes, is shown to develop electrical excitability and greatly increased acetylcholine sensitivity in the presence of NGF, implying that NGF influences the physiological differentiation of certain neural crest derivatives.
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The Biology and Mechanism of Action of Nerve Growth Factor
Bruce A. Yankner,Eric M. Shooter +1 more
TL;DR: The role of NGF and Neuronal Survival, and the Mechanism of action, are examined in the context of the development of the nervous system.