Journal ArticleDOI
Sarcoma viruses carrying ras oncogenes induce differentiation-associated properties in a neuronal cell line
Makoto Noda,Minoru S.H. Ko,Akihiko Ogura,Ding Gan Liu,Takehiko Amano,Toshiya Takano,Yoji Ikawa +6 more
TLDR
It is found that Ki- and Ha-MSV mimic some, if not all, of the activities of NGF in PC12 cells, and there is evidence that the viral oncogenes, v-Ki-ras and v-Ha-ras, are responsible for this phenomenon.Abstract:
The growth-promoting and/or differentiation-blocking activities of Kirsten (Ki-MSV) or Harvey murine sarcoma virus (Ha-MSV) on various types of cells in vitro are well documented. Here we report an unexpected effect of these viruses on a rat phaeochromocytoma cell line, PC12. PC12 cells, which multiply indefinitely in growth medium, are known to respond to nerve growth factor (NGF) by cessation of cell division and expression of several properties resembling those of differentiated sympathetic neurones. We have found that Ki- and Ha-MSV mimic some, if not all, of the activities of NGF in PC12 cells, and there is evidence that the viral oncogenes, v-Ki-ras and v-Ha-ras, are responsible for this phenomenon. This system may be of value for studying the mechanism of action of the v-ras genes as well as the regulatory mechanism of growth and differentiation in neuronal cells.read more
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Expression of DRG during murine embryonic development.
TL;DR: Analysis of drg mRNA and protein in adult mouse tissues and various cell lines of different origins indicated that it is expressed widely, albeit at low and variable levels, suggesting that DRG may play multiple roles in development and normal cell metabolism.
Journal ArticleDOI
Targeting SMN to Cajal bodies and nuclear gems during neuritogenesis
TL;DR: It is shown that the number and composition of the nuclear subdomains called Cajal bodies and gems changes during the course of N-ras-induced neuritogenesis in the PC12-derived cell line UR61, and that gems clearly represent a distinct category of nuclear body.
Journal ArticleDOI
Nerve growth factor-dependent activation of the small GTPase Rin.
TL;DR: It is shown that stimulation of PC6 cells with either epidermal growth factor or nerve growth factor results in rapid activation of Rin, and this activation correlates with the onset of Ras activation, and dominant-negative Ras completely inhibits Rin activation induced by NGF.
Journal ArticleDOI
Characterization of GFR, a novel guanine nucleotide exchange factor for Rap1.
TL;DR: GFR shows close sequence similarity to EPAC/cAMP‐GEFI/II although GFR lacks a cAMP binding domain and contains a nuclear localization signal, and northern blot analysis suggested that GFR mRNA is strongly expressed in the brain.
Journal Article
Expression of Hepatocyte and Oval Cell Antigens in Hepatocellular Carcinomas Produced by Oncogene-transfected Liver Epithelial Cells
TL;DR: In addition to its tumorigenic effect, the EJ oncogene induced the differentiation of tumor cells toward the hepatocyte lineage, which provides further confirmation that oval cells can serve as progenitors of differentiated hepatocellular carcinomas.
References
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Journal ArticleDOI
A new and rapid colorimetric determination of acetylcholinesterase activity.
TL;DR: A photometric method for determining acetylcholinesterase activity of tissue extracts, homogenates, cell suspensions, etc., has been described and Kinetic constants determined by this system for erythrocyte eholinesterases are presented.
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Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor.
TL;DR: A single cell clonal line which responds reversibly to nerve growth factor (NGF) has been established from a transplantable rat adrenal pheochromocytoma and should be a useful model system for neurobiological and neurochemical studies.
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In yeast, RAS proteins are controlling elements of adenylate cyclase
T. Toda,Isao Uno,Tatsuo Ishikawa,Scott Powers,Tohru Kataoka,Daniel Broek,Scott J. Cameron,James R. Broach,Kunihiro Matsumoto,Michael Wigler +9 more
TL;DR: S. cerevisiae strains containing RAS2val19, a Ras2 gene with a missense mutation analogous to one that activates the transforming potential of mammalian ras genes, have growth and biochemical properties strikingly similar to yeast strains carrying IAC or bcy1.
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Nerve growth factor-induced increase in electrical excitability and acetylcholine sensitivity of a rat pheochromocytoma cell line
TL;DR: A cloned line of rat pheochromocytoma cells (PC12) which responds to nerve growth factor by cessation of division and extension of neurone-like processes, is shown to develop electrical excitability and greatly increased acetylcholine sensitivity in the presence of NGF, implying that NGF influences the physiological differentiation of certain neural crest derivatives.
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The Biology and Mechanism of Action of Nerve Growth Factor
Bruce A. Yankner,Eric M. Shooter +1 more
TL;DR: The role of NGF and Neuronal Survival, and the Mechanism of action, are examined in the context of the development of the nervous system.