SARS-CoV-2 RNA in wastewater anticipated COVID-19 occurrence in a low prevalence area.
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TLDR
The detection of SARS-CoV-2 in wastewater in early stages of the spread of COVID-19 highlights the relevance of this strategy as an early indicator of the infection within a specific population.About:
This article is published in Water Research.The article was published on 2020-05-16 and is currently open access. It has received 881 citations till now. The article focuses on the topics: Porcine epidemic diarrhea virus.read more
Citations
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Journal ArticleDOI
First environmental surveillance for the presence of SARS-CoV-2 RNA in wastewater and river water in Japan.
TL;DR: Comparison with the reported COVID-19 cases in Yamanashi Prefecture showed that SARS-CoV-2 RNA was detected in the secondary-treated wastewater sample when the cases peaked in the community.
Journal ArticleDOI
Temporal Detection and Phylogenetic Assessment of SARS-CoV-2 in Municipal Wastewater.
Artem Nemudryi,Anna Nemudraia,Tanner Wiegand,Kevin Surya,Murat Buyukyoruk,Calvin Cicha,Karl K Vanderwood,Royce A. Wilkinson,Blake Wiedenheft +8 more
TL;DR: It is shown that changes in SARS-CoV-2 RNA concentrations follow symptom onset gathered by retrospective interview of patients but precedes clinical test results, and how genome sequencing can be used for genotyping viral strains circulating in a community.
Journal ArticleDOI
Presence and infectivity of SARS-CoV-2 virus in wastewaters and rivers.
Sara Giordana Rimoldi,Fabrizio Stefani,Anna Gigantiello,Stefano Polesello,Francesco Comandatore,Davide Mileto,Mafalda Maresca,Concetta Longobardi,Alessandro Mancon,Francesca Romeri,Cristina Pagani,Francesca Cappelli,Claudio Roscioli,Lorenzo Moja,Maria Rita Gismondo,Franco Salerno +15 more
TL;DR: Risks for public health should be limited, although a precautionary approach to risk assessment is here advocated, giving the preliminary nature of the presented data.
Journal ArticleDOI
First detection of SARS-CoV-2 RNA in wastewater in North America: A study in Louisiana, USA.
Samendra P. Sherchan,Shalina Shahin,Lauren M. Ward,Sarmila Tandukar,Tiong Gim Aw,Bradley W. Schmitz,Warish Ahmed,Masaaki Kitajima +7 more
TL;DR: To the knowledge, this is the first study reporting the detection of SARS-CoV-2 RNA in wastewater in North America, including the USA, however, concentration methods and RT-qPCR assays need to be refined and validated to increase the sensitivity of Sars- CoV- 2 RNA detection in wastewater.
Journal ArticleDOI
Comparison of virus concentration methods for the RT-qPCR-based recovery of murine hepatitis virus, a surrogate for SARS-CoV-2 from untreated wastewater.
Warish Ahmed,Paul M. Bertsch,Aaron Bivins,Kyle Bibby,Kata Farkas,Amy Gathercole,Eiji Haramoto,Pradip Gyawali,Asja Korajkic,Brian R. McMinn,Jochen F. Mueller,Stuart L. Simpson,Wendy Smith,Erin M. Symonds,Kevin V. Thomas,Rory Verhagen,Masaaki Kitajima +16 more
TL;DR: The results suggest that absorption-extraction methods with minimal pretreatment or without manipulation can provide suitably rapid, cost-effective and relatively straightforward recovery of enveloped viruses in wastewater.
References
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Journal ArticleDOI
Inactivation of an Enveloped Surrogate Virus in Human Sewage
Lisa M. Casanova,Scott R. Weaver +1 more
TL;DR: It is suggested that enveloped viruses can undergo 6–7 log inactivation in sewage in 3–7 days, depending on temperature, and longer holding times may be desirable out of an abundance of caution at lower temperatures.
Journal ArticleDOI
Stability of SARS-CoV-2 in different environmental conditions – Authors' reply
Alex W.H. Chin,Leo L.M. Poon +1 more
Journal ArticleDOI
Comparison and evaluation of conventional RT-PCR, SYBR green I and TaqMan real-time RT-PCR assays for the detection of porcine epidemic diarrhea virus.
Xinrong Zhou,Tiansheng Zhang,Deping Song,Tao Huang,Qi Peng,Yanjun Chen,Anqi Li,Fanfan Zhang,Qiong Wu,Yu Ye,Yuxin Tang +10 more
TL;DR: With the features of high specificity, sensitivity, and reproducibility, the TaqMan real-time RT-PCR established in this study could be a useful tool for clinical diagnosis, epidemiological surveys and outbreak investigations of PED.
Posted ContentDOI
ddPCR: a more sensitive and accurate tool for SARS-CoV-2 detection in low viral load specimens
Tao Suo,Xinjin Liu,Jiangpeng Feng,Ming Guo,Wenjia Hu,Dong Guo,Hafiz Ullah,Yang Yang,Qiuhan Zhang,Xin Wang,Muhanmmad Sajid,Zhixiang Huang,Liping Deng,Tielong Chen,Fang Liu,Ke Xu,Yuan Liu,Qi Zhang,Yingle Liu,Yong Xiong,Guozhong Chen,Ke Lan,Yu Chen +22 more
TL;DR: In this paper, an optimized droplet digital PCR (ddPCR) was used for detection of SARS-CoV-2, which showed that the limit of detection of ddPCR is significantly lower than that of RT-PCR.
Journal ArticleDOI
Detection of Hepatitis E Virus in Sewage After an Outbreak on a French Island.
Takayuki Miura,Takayuki Miura,Sébastien Lhomme,Jean-Claude Le Saux,Philippe Le Mehaute,Yvonnick Guillois,Elizabeth Couturier,Jacques Izopet,Florence Abranavel,Françoise S. Le Guyader +9 more
TL;DR: Investigating the routes of transmission of HEV, including the potential for sewage effluent to contain infectious HeV, may help to better understand the epidemiology of this pathogen, which is considered to be an emerging concern in Europe.
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