Journal ArticleDOI
Sequence and gene content in 52 kb including and centromeric to the G6PD gene in Xq28
Massimo Zollo,Richard Mazzarella,Silvia Bione,Daniela Toniolo,David Schlessinger,Michele D'Urso,Ellson Y. Chen +6 more
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TLDR
In segments of unique sequence, computer-aided analysis predicted three possible genes, one of which has thus far been confirmed by the recovery of a corresponding cDNA, both by a direct hybridization method and by a PCR-based method based on a primer pair inferred from the genomic sequence.Abstract:
A cosmid containing 36.4 kb of high GC human DNA centromeric to the G6PD gene has been analyzed. The sequence was 99.9% precise, based on the comparison of 4.3 kb that overlaps an earlier analysis of 20.1 kb containing G6PD. Properties of the entire 52 kb region that may be characteristic of high GC portions of the genome include a very high density of sixty-two half or full Alu sequences, or 1.2/kb, and an absence of L1 sequences. Other highly repetitive sequences include 11 MER sequences, one of them interrupted at two positions by groups of 3 Alu elements. In segments of unique sequence, computer-aided analysis predicted three possible genes, one of which has thus far been confirmed by the recovery of a corresponding cDNA, both by a direct hybridization method and by a PCR-based method based on a primer pair inferred from the genomic sequence. The cDNA has been sequenced, and is completely concordant with counterpart genomic sequence; it has no resemblance to any previously described gene.read more
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Densely methylated sequences that are preferentially localized at telomere-proximal regions of human chromosomes.
TL;DR: A library of densely methylated DNA sequences from human blood DNA is constructed by selecting fragments with a high affinity for a methyl-CpG binding domain (MBD) column and analysis of the methylation status of random clones indicated that all were heavily methylated at CpGs in blood DNA, but many were under-methylated in sperm DNA.
Journal ArticleDOI
Long-Range Sequence Analysis in Xq28: Thirteen Known and Six Candidate Genes in 219.4 kb of High GC DNA Between the RCP/GCP and G6PD Loci
Ellson Y. Chen,Massimo Zollo,Richard Mazzarella,Alfredo Ciccodicola,Chun-Nan Chen,Lin Zuo,Cheryl R. Heiner,Monica Repetto,F. Burough,David Schlessinger,Michele D'Urso +10 more
TL;DR: DNA comprising 219 447 bp was sequenced in nine cosmids and verified at > 99.9% precision, showing the orientation of transcription varies among the first eight genes, then runs centromeric to telomeric for the next five, and is in the opposite sense for the last six.
Journal ArticleDOI
Genomic sequence, structural organization, molecular evolution, and aberrant rearrangement of promyelocytic leukemia zinc finger gene.
T. Zhang,H. Xiong,L.-X. Kan,C.-K. Zhang,X.-F. Jiao,Gang Fu,Gang Fu,Qun-Ye Zhang,L. Lu,Jian-Hua Tong,Bai-Wei Gu,Ming Yu,Jia Liu,Jonathan D. Licht,Samuel Waxman,A Zelent,Ellson Y. Chen,Ellson Y. Chen,Shunle Chen +18 more
TL;DR: A 201-kilobase genomic DNA region containing the entire PLZF gene was sequenced and the presence of two MLL/PLZF-like alignments on human chromosome 11q23 and 19 suggests a syntenic replication during evolution.
Journal ArticleDOI
Sequence-based exon prediction around the synaptophysin locus reveals a gene-rich area containing novel genes in human proximal Xp.
Simon E. Fisher,Alfredo Ciccodicola,Karo Tanaka,Anna Curci,Sonia Desicato,Michele D'Urso,Ian W. Craig +6 more
TL;DR: The complete sequencing of a SYP-containing cosmid revealed the presence of 29 putative exons, organized into three genes, in addition to the 7 exons of the complete SYP coding region, all mapping within a 44-kb interval.
Journal ArticleDOI
Drosomycin-like defensin, a human homologue of Drosophila melanogaster drosomycin with antifungal activity.
Anna Simon,Bart Jan Kullberg,Brian Tripet,Otto C. Boerman,Patrick L.J.M. Zeeuwen,Johanna van der Ven-Jongekrijg,Paul E. Verweij,Joost Schalkwijk,Robert S. Hodges,Jos W. M. van der Meer,Mihai G. Netea +10 more
TL;DR: The expression of DLD mRNA in several human tissues, particularly in the skin, consistent with its putative role as a defensin against invading microorganisms is shown, the first indication of an endogenous human peptide with specific antifungal activity.
References
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Basic Local Alignment Search Tool
TL;DR: A new approach to rapid sequence comparison, basic local alignment search tool (BLAST), directly approximates alignments that optimize a measure of local similarity, the maximal segment pair (MSP) score.
Journal ArticleDOI
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity
TL;DR: A technique for conveniently radiolabeling DNA restriction endonuclease fragments to high specific activity is described, and these "oligolabeled" DNA fragments serve as efficient probes in filter hybridization experiments.
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity
TL;DR: In this article, a technique for conveniently radiolabeling DNA restriction endonuclease fragments to high specific activity is described, where DNA fragments are purified from agarose gels directly by ethanol precipitation and are then denatured and labeled with the large fragment of DNA polymerase I, using random oligonucleotides as primers.
Journal ArticleDOI
Complementary DNA sequencing : expressed sequence tags and human genome project
Mark Raymond Adams,Jenny M. Kelley,Jeannine D. Gocayne,Mark Dubnick,Mihael H. Polymeropoulos,Hong Xiao,Carl R. Merril,Andrew Wu,Bjorn Olde,Ruben F. Moreno,Anthony R. Kerlavage,W. Richard McCombie,J. Craig Venter +12 more
TL;DR: Automated partial DNA sequencing was conducted on more than 600 randomly selected human brain complementary DNA (cDNA) clones to generate expressed sequence tags (ESTs), which will facilitate the tagging of most human genes in a few years at a fraction of the cost of complete genomic sequencing.
Journal ArticleDOI
2.2 Mb of contiguous nucleotide sequence from chromosome III of C. elegans
Richard K. Wilson,R. Ainscough,Karen E. Anderson,C. Baynes,Mary Berks,James K. Bonfield,James Burton,M. Connell,T. Copsey,John A. Cooper,Alan Coulson,M. Craxton,S. Dear,Zijin Du,Richard Durbin,Anthony Favello,A. Fraser,Lucinda Fulton,A. Gardner,Philip Green,T. Hawkins,LaDeana W. Hillier,M. Jier,L. Johnston,Martin K. Jones,J. Kershaw,J. Kirsten,N. Laisster,Phil Latreille,J. Lightning,C. Lloyd,Beverley J. Mortimore,M. O'Callaghan,J. Parsons,C. Percy,L. Rifken,A. Roopra,D. Saunders,Ratna Shownkeen,M. Sims,N. Smaldon,Andrew J.H. Smith,Michael D. Smith,Erik L. L. Sonnhammer,Rodger Staden,John Sulston,Jean Thierry-Mieg,K. Thomas,M. Vaudin,K. Vaughan,Robert H. Waterston,A. Watson,L. Weinstock,J. Wilkinson-Sproat,P. Wohldman +54 more
TL;DR: The nucleotide sequence of a contiguous 2,181,032 base pairs in the central gene cluster of chromosome III is completed, and comparison with the public sequence databases reveals similarities to previously known genes for about one gene in three.