Signaling-mediated bacterial persister formation
Nicole M. Vega,Kyle R. Allison,Kyle R. Allison,Ahmad S. Khalil,Ahmad S. Khalil,James J. Collins +5 more
TLDR
It is shown that bacterial communication through indole signaling induces persistence, a phenomenon in which a subset of an isogenic bacterial population tolerates antibiotic treatment, and a model in which indoles signaling “inoculates” a bacterial sub-population against antibiotics by activating stress responses, leading to persister formation is proposed.Abstract:
Here we show that bacterial communication through indole signaling induces persistence, a phenomenon in which a subset of an isogenic bacterial population tolerates antibiotic treatment. We monitor indole-induced persister formation using microfluidics and identify the role of oxidative-stress and phage-shock pathways in this phenomenon. We propose a model in which indole signaling 'inoculates' a bacterial subpopulation against antibiotics by activating stress responses, leading to persister formation.read more
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The Application of Ecological Theory Toward an Understanding of the Human Microbiome
Elizabeth K. Costello,Keaton Stagaman,Les Dethlefsen,Brendan J. M. Bohannan,David A. Relman,David A. Relman +5 more
TL;DR: This work explores three core scenarios of human microbiome assembly: development in infants, representing assembly in previously unoccupied habitats; recovery from antibiotics, representingassembly after disturbance; and invasion by pathogens, representingAssembly in the context of invasive species.
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Biofilm-Related Infections: Bridging the Gap between Clinical Management and Fundamental Aspects of Recalcitrance toward Antibiotics
TL;DR: This review presents the current understanding of the molecular mechanisms of biofilm recalcitrance toward antibiotics and describes how recent progress has improved the capacity to design original and efficient strategies to prevent or eradicate biofilm-related infections.
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Biofilm infections, their resilience to therapy and innovative treatment strategies
Ute Römling,Carlos Balsalobre +1 more
TL;DR: Cyclic di‐nucleotides are also potential immunotherapeutic agents to treat antibiotic‐resistant bacterial infections and might be appropriate targets for antibiofilm therapy in combination with antibiotic treatment strategies.
Journal ArticleDOI
Definitions and guidelines for research on antibiotic persistence
Naomi N.Q. Balaban,Sophie Helaine,Kim Lewis,Martin Ackermann,Martin Ackermann,Bree B. Aldridge,Dan I. Andersson,Mark P. Brynildsen,Dirk Bumann,Andrew Camilli,James J. Collins,James J. Collins,James J. Collins,Christoph Dehio,Sarah M. Fortune,Jean-Marc Ghigo,Wolf-Dietrich Hardt,Alexander Harms,Matthias Heinemann,Deborah T. Hung,Urs Jenal,Bruce R. Levin,Jan Michiels,Gisela Storz,Man-Wah Tan,Tanel Tenson,Laurence Van Melderen,Annelies S. Zinkernagel +27 more
TL;DR: Scientists working on the response of bacteria to antibiotics define antibiotic persistence and provide practical guidance on how to study bacterial persister cells, and provide a guide to measuring persistence.
Journal ArticleDOI
Silver Enhances Antibiotic Activity Against Gram-Negative Bacteria
Jose Ruben Morones-Ramirez,Jose Ruben Morones-Ramirez,Jonathan A. Winkler,Jonathan A. Winkler,Catherine S. Spina,Catherine S. Spina,James J. Collins +6 more
TL;DR: This work shows that silver can be used to enhance the action of existing antibiotics against Gram-negative bacteria, thus strengthening the antibiotic arsenal for fighting bacterial infections.
References
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Journal ArticleDOI
Bacterial Persistence as a Phenotypic Switch
Nathalie Q. Balaban,Nathalie Q. Balaban,Jack Merrin,Remy Chait,Lukasz Kowalik,Stanislas Leibler +5 more
TL;DR: Investigating the persistence of single cells of Escherichia coli with the use of microfluidic devices found phenotypic switching occurred between normally growing cells and persister cells having reduced growth rates, leading to a simple mathematical description of the persistence switch.
Journal ArticleDOI
Persister cells, dormancy and infectious disease
TL;DR: The molecular mechanisms that underlie the formation of dormant persister cells are now being unravelled and are the focus of this Review.
Journal ArticleDOI
Persister cells and tolerance to antimicrobials.
TL;DR: The data indicate that persisters are specialized survivor cells that are not at a particular stage in the cell cycle, neither are they defective cells nor cells created in response to antibiotics.
Journal ArticleDOI
Metabolite-enabled eradication of bacterial persisters by aminoglycosides
Kyle R. Allison,Mark P. Brynildsen,Mark P. Brynildsen,James J. Collins,James J. Collins,James J. Collins +5 more
TL;DR: It is shown that specific metabolic stimuli enable the killing of both Gram-negative and Gram-positive bacterial persisters with aminoglycosides, and this approach can improve the treatment of chronic infections in a mouse urinary tract infection model.
Journal ArticleDOI
hipA, a newly recognized gene of Escherichia coli K-12 that affects frequency of persistence after inhibition of murein synthesis.
H S Moyed,K P Bertrand +1 more
TL;DR: Transposons Tn5 and Tn10 have been inserted close to hipA making it possible to explore the molecular genetics of persistence, a long recognized but poorly understood phenomenon.