Single Cell Encapsulation via Pickering Emulsion for Biopesticide Applications
Noga Yaakov,Karthik Ananth Mani,Reut Felfbaum,Magen Lahat,Noam Da Costa,Eduard Belausov,Dana Ment,Guy Mechrez +7 more
- Vol. 3, Iss: 10, pp 14294-14301
Reads0
Chats0
TLDR
The Pickering emulsion-based formulation exhibited significantly higher pest control activity against Spodoptera littoralis larvae compared to the control systems, thus making it a promising, cost-effective, innovative approach for tackling the pest control challenge.Abstract:
A new approach for single cell microencapsulation in an oil-in-water (o/w) Pickering emulsion is presented. The water/paraffin emulsions were stabilized by amine-functionalized silica nanoparticles. The droplet size of the emulsions was highly tunable, and ranged from 1 to 30 μm in diameter. The controllable droplet size along with the high colloidal stability of the Pickering emulsionswas harnessed to obtain single cell microencapsulation. Successful encapsulation of the conidia entomopathogenic fungus Metarhizium brunneum by the studied Pickering emulsions was confirmed via confocal laser scanning microscopy. The resulting systems were implemented to develop a novel biopesticide formulation for arthropod pest control. The conidia incorporated in the emulsions were applied to Ricinus communis leaves by spray assay. After drying of the emulsion, a silica-based honeycomb-like structure with an ordered hierarchical porosity is formed. This structure preserves the individual cell encapsulation. The successful single cell encapsulation has led to a high distribution of conidia cells on the leaves. The Pickering emulsion-based formulation exhibited significantly higher pest control activity against Spodoptera littoralis larvae compared to the control systems, thus making it a promising, cost-effective, innovative approach for tackling the pest control challenge.read more
Citations
More filters
Microscale Strategies for Generating Cell-Encapsulating Hydrogels
TL;DR: Common hydrogels and their crosslinking methods are introduced and the latest microscale approaches for generation of cell containing gel particles are reviewed, specifically on microfluidics-based methods and on techniques such as micromolding and electrospinning.
Journal ArticleDOI
Cannabis compounds exhibit anti-inflammatory activity in vitro in COVID-19-related inflammation in lung epithelial cells and pro-inflammatory activity in macrophages.
Seegehalli M Anil,Nurit Shalev,Ajjampura C. Vinayaka,Stalin Nadarajan,Dvora Namdar,Eduard Belausov,Irit Shoval,Karthik Ananth Mani,Guy Mechrez,Hinanit Koltai +9 more
TL;DR: In this article, an extract fraction from C. sativa Arbel strain (FCBD) substantially reduced interleukin (IL)-6 and -8 levels in an alveolar epithelial (A549) cell line.
Journal ArticleDOI
Clay Nanotube Liquid Marbles Enhanced with Inner Biofilm Formation for the Encapsulation and Storage of Bacteria at Room Temperature
Abhishek Panchal,Naureen Rahman,Svetlana A. Konnova,Rawil Fakhrullin,Rawil Fakhrullin,Rawil Fakhrullin,Donghui Zhang,Diane A. Blake,Vijay T. John,Evgenii Ivanov,Yuri Lvov,Yuri Lvov +11 more
TL;DR: In this paper, liquid marbles were created with water droplets encapsulated by a layer of clay nanotubes, and they were shown to be able to be used to create marbles from oil-in-water Pickering emulsion.
Journal ArticleDOI
UV sensitivity of Beauveria bassiana and Metarhizium anisopliae isolates under investigation as potential biological control agents in South African citrus orchards.
TL;DR: Findings indicate that a suitable UV protectant formulation of these fungi or a different application strategy will be required for success against P. citri and S. aurantii.
Journal ArticleDOI
Encapsulation Strategies for Bacillus thuringiensis: From Now to the Future.
Jhones Luiz de Oliveira,Leonardo Fernandes Fraceto,Alejandra Bravo,Ricardo Antonio Polanczyk +3 more
TL;DR: In this article, a review describes current studies and recent discoveries related to Bacillus thuringiensis (Bt) and processes for the encapsulation of Bt derivatives, such as Cry pesticidal proteins.
References
More filters
Journal ArticleDOI
Fiji: an open-source platform for biological-image analysis
Johannes Schindelin,Ignacio Arganda-Carreras,Erwin Frise,Verena Kaynig,Mark Longair,Tobias Pietzsch,Stephan Preibisch,Curtis Rueden,Stephan Saalfeld,Benjamin Schmid,Jean-Yves Tinevez,Daniel J. White,Volker Hartenstein,Kevin W. Eliceiri,Pavel Tomancak,Albert Cardona +15 more
TL;DR: Fiji is a distribution of the popular open-source software ImageJ focused on biological-image analysis that facilitates the transformation of new algorithms into ImageJ plugins that can be shared with end users through an integrated update system.
Journal ArticleDOI
A method of computing the effectiveness of an insecticide
TL;DR: In order to make experimental studies comparable and statistically meaningful, the article recommends the following formula: per cent control = 100(X - Y)/X, which eliminates errors due to deaths in the control sample which were not due to the insecticide.
Journal ArticleDOI
Mycoinsecticides and Mycoacaricides: A comprehensive list with worldwide coverage and international classification of formulation types ☆
TL;DR: An updated, comprehensive list of mycoinsecticides and mycoacaricides developed worldwide since the 1960s, with a total of 171 products claimed to control acarines (mites and ticks) in at least 4 families.
Journal ArticleDOI
Ordered macroporous materials by emulsion templating
Arnout Imhof,David J. Pine +1 more
TL;DR: In this paper, a method for producing highly monodisperse macroporous materials with pore sizes ranging from 50nm to several micrometres is presented. But the pore size can be accurately controlled, and the technique should be applicable to a wide variety of metal oxides and even organic polymer gels.
Journal ArticleDOI
Pickering Emulsions Stabilized by Monodisperse Latex Particles: Effects of Particle Size
B. P. Binks and,S. O. Lumsdon +1 more
TL;DR: The preparation, type, and stability of emulsions of oil and water stabilized solely by spherical, monodisperse polystyrene latex particles of different sizes is described in this paper.