Journal ArticleDOI
Specific Covalent Labeling of Recombinant Protein Molecules Inside Live Cells
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TLDR
This system provides a recipe for slightly modifying a target protein so that it can be singled out from the many other proteins inside live cells and fluorescently stained by small nonfluorescent dye molecules added from outside the cells.Abstract:
Recombinant proteins containing four cysteines at the i , i + 1, i + 4, and i + 5 positions of an α helix were fluorescently labeled in living cells by extracellular administration of 4′,5′-bis(1,3,2-dithioarsolan-2-yl)fluorescein. This designed small ligand is membrane-permeant and nonfluorescent until it binds with high affinity and specificity to the tetracysteine domain. Such in situ labeling adds much less mass than does green fluorescent protein and offers greater versatility in attachment sites as well as potential spectroscopic and chemical properties. This system provides a recipe for slightly modifying a target protein so that it can be singled out from the many other proteins inside live cells and fluorescently stained by small nonfluorescent dye molecules added from outside the cells.read more
Citations
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Journal ArticleDOI
Effects of flanking sequences and cellular context on subcellular behavior and pathology of mutant HTT.
Anjalika Chongtham,Anjalika Chongtham,Douglas J. Bornemann,Brett A. Barbaro,Tamas Lukacsovich,Namita Agrawal,Adeela Syed,Shane A. Worthge,Judith Purcell,John Burke,Theodore M Chin,J. Lawrence Marsh +11 more
TL;DR: The influence of sequences outside the expanded polyQ domain is revealed by finding that small modifications to the HTT118 or HTT171 fragments can dramatically alter their subcellular behavior and pathogenicity.
Journal ArticleDOI
Controlling the active properties of excitable cells
Kurt W. Marek,Graeme W. Davis +1 more
TL;DR: This work has shown that the ability to experimentally manipulate the active properties of neurons in vivo contributes to a detailed understanding of how individual neurons participate in the circuitry that controls behavior.
Journal ArticleDOI
A Supramolecular Approach to Protein Labeling. A Novel Fluorescent Bioassay for Concanavalin A Activity
TL;DR: A new method for the bioassay of concanavalin A is based on the interaction of saccharide-substituted oligopyrrolic macrocycles with lectins, which leads to the release of the label and partial quenching of fluorescence due to reaggregation.
Journal ArticleDOI
A SNAP-tag fluorogenic probe mimicking the chromophore of the red fluorescent protein Kaede
Kwan Ho Jung,Matthew Fares,Leeann S. Grainger,Charles H. Wolstenholme,Anna Hou,Yu Liu,Xin Zhang +6 more
TL;DR: A SNAP-tag fluorogenic probe 4c mimicking the chromophore of the red fluorescent protein Kaede is reported, such that conjugation with SNAPf protein leads to inhibition of twisted intramolecular charge transfer, triggering fluorogenecity.
Journal ArticleDOI
Versatile Interacting Peptide (VIP) Tags for Labeling Proteins with Bright Chemical Reporters.
TL;DR: A new set of small, selective, genetically encoded tags for proteins based on a heterodimeric coiled‐coil interaction between two peptides: CoilY and CoilZ are described, which allowed rapid and specific delivery of bright organic dyes or quantum dots to proteins displayed on living cells.
References
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Journal ArticleDOI
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Journal ArticleDOI
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