Studies of in vitro transcription by calf thymus RNA polymerase II using a novel duplex DNA template.
T R Kadesch,M J Chamberlin +1 more
Reads0
Chats0
TLDR
The transcriptional elongation reaction carried out by calf thymus RNA polymerase II in vitro differs in several respects from that which must take place in vivo.About:
This article is published in Journal of Biological Chemistry.The article was published on 1982-05-10 and is currently open access. It has received 174 citations till now. The article focuses on the topics: Polymerase & RNA-dependent RNA polymerase.read more
Citations
More filters
Journal ArticleDOI
Structural Basis of Transcription: An RNA Polymerase II Elongation Complex at 3.3 Å Resolution
TL;DR: The crystal structure of RNA polymerase II in the act of transcription was determined at 3.3 Å resolution and protein–nucleic acid contacts help explain DNA and RNA strand contacts, the specificity of RNA synthesis, “abortive cycling” during transcription initiation, and RNA and DNA translocation during transcription elongation.
Journal ArticleDOI
Inactivation of the SR Protein Splicing Factor ASF/SF2 Results in Genomic Instability
Xialu Li,James L. Manley +1 more
TL;DR: It is shown that in vivo depletion of ASF/SF2 results in a hypermutation phenotype likely due to DNA rearrangements, reflected in the rapid appearance of DNA double-strand breaks and high-molecular-weight DNA fragments, and this results support a model by which recruitment of ASFs to nascent transcripts by RNA polymerase II prevents formation of mutagenic R loop structures.
Journal ArticleDOI
Nucleosomes inhibit the initiation of transcription but allow chain elongation with the displacement of histones
TL;DR: In the course of readthrough transcription, the histones were displaced from the DNA, as shown by the exposure of restriction sites and by a shift of the template to the position of naked DNA in a gel.
Journal ArticleDOI
Basic Mechanisms of Transcript Elongation and Its Regulation
TL;DR: New models for the structure of ternary complexes, and for the mechanism by which they move along DNA, provide plausible explanations for novel biochemical reactions that have been observed and offer the prospect of understanding many significant biological regulatory systems at the molecular level.
Journal ArticleDOI
Nucleosome Remodeling Induced by RNA Polymerase II: Loss of the H2A/H2B Dimer during Transcription
Maria L. Kireeva,Wendy Walter,Vladimir Tchernajenko,Vladimir A. Bondarenko,Mikhail Kashlev,Vasily M. Studitsky +5 more
TL;DR: The mechanisms for transcription through the nucleosome by Pol II and SP6 RNAP are clearly different, and Pol II leaves behind an imprint of disrupted chromatin structure.
References
More filters
Journal ArticleDOI
Detection of specific sequences among DNA fragments separated by gel electrophoresis.
TL;DR: This paper describes a method of transferring fragments of DNA from agarose gels to cellulose nitrate filters that can be hybridized to radioactive RNA and hybrids detected by radioautography or fluorography.
Book ChapterDOI
[31] Electron microscope heteroduplex methods for mapping regions of base sequence homology in nucleic acids
Journal ArticleDOI
Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels.
TL;DR: Electrophoresis in alkaline gels can provide accurate molecular weights for linear, single-Stranded DNAs, and should be useful in analyzing DNA for single-strand breaks, depurinations or topological differences such as ring forms.
Journal ArticleDOI
DNA-dependent transcription of adenovirus genes in a soluble whole-cell extract
TL;DR: A cell-free system for studying the synthesis of mRNA in mammalian cells, which consists of a dialyzed and concentrated whole-cell extract derived from HeLa cells, small molecules and cofactors needed for transcription, and exogenously added DNA.
Journal ArticleDOI
Attenuation in the control of expression of bacterial operons
TL;DR: Bacterial operons concerned with the biosynthesis of amino acids are often controlled by a process of attenuation, where the amino acid in short supply means translation is stalled at the relevant codons of the transcript long enough for the succeeding segment of the transcripts to form secondary structures that allow the transcribing RNA polymerase molecule to proceed through a site that otherwise dictates termination of transcription.
Related Papers (5)
Factors involved in specific transcription by mammalian RNA polymerase II. Transcription factor IIS stimulates elongation of RNA chains
Danny Reinberg,Robert G. Roeder +1 more