Journal ArticleDOI
Subchromosomal Positioning of the Epidermal Differentiation Complex (EDC) in Keratinocyte and Lymphoblast Interphase Nuclei
TLDR
This study of the epidermal differentiation complex at 1q21 provides a further example of a gene-dense complex capable of assuming extraterritorial positioning in relation to cell type/transcription status.About:
This article is published in Experimental Cell Research.The article was published on 2002-01-15. It has received 206 citations till now. The article focuses on the topics: Cellular differentiation & Chromosome Territory.read more
Citations
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Journal ArticleDOI
Beyond the sequence : Cellular organization of genome function
TL;DR: The functional relevance of spatial and temporal genome organization at three hierarchical levels: the organization of nuclear processes, the higher-order organization of the chromatin fiber, and the spatial arrangement of genomes within the cell nucleus are discussed.
Journal ArticleDOI
Dynamic genome architecture in the nuclear space: regulation of gene expression in three dimensions
TL;DR: This work has shown that the dynamic nature of the positioning of genetic material in the nuclear space and the higher-order architecture of the nucleus are integrated is essential to the overall understanding of gene regulation.
Journal ArticleDOI
Nuclear organization of the genome and the potential for gene regulation
Peter Fraser,Wendy A. Bickmore +1 more
TL;DR: Technological advances are now helping to identify the spatial relationships and interactions of genes and regulatory elements in the nucleus and are revealing an unexpectedly extensive network of communication within and between chromosomes.
Journal ArticleDOI
Chromatin decondensation and nuclear reorganization of the HoxB locus upon induction of transcription
TL;DR: It is suggested that higher-order chromatin structure regulates the expression of the HoxB cluster at several levels, and choreographed looping out of decondensed chromatin from chromosome territories may allow for activation of high levels of transcription from the sequence of genes along the cluster.
Journal ArticleDOI
Chromosome territories--a functional nuclear landscape.
TL;DR: This work has shown that chromosomes in the cell nucleus are organized as chromosome territories, and present models of chromosome territory architecture differ widely with respect to the possible functional implications of dynamic changes of this architecture during the cell cycle and terminal cell differentiation.
References
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Journal ArticleDOI
Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction
TL;DR: A new method of total RNA isolation by a single extraction with an acid guanidinium thiocyanate-phenol-chloroform mixture is described, providing a pure preparation of undegraded RNA in high yield and can be completed within 4 h.
Book
A Laboratory manual
TL;DR: To develop a program to print the barcodes using two commonly uses command sets and hence evaluates their ease of use for such applications, students should be able to program dot matrix printers, by manipulating bit level information and ink jet printers using page description language, such as PCL.
Journal ArticleDOI
Differences in the localization and morphology of chromosomes in the human nucleus
TL;DR: It is demonstrated that the distribution of genomic sequences between chromosomes has implications for nuclear structure and the findings are discussed in relation to a model of the human nucleus that is functionally compartmentalized.
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Association of Transcriptionally Silent Genes with Ikaros Complexes at Centromeric Heterochromatin
Karen E. Brown,Simon Guest,Stephen T. Smale,Kyungmin Hahm,Matthias Merkenschlager,Amanda G. Fisher +5 more
TL;DR: It is shown that transcriptionally inactive but not transcriptionally active genes associate with Ikaros-heterochromatin foci, which support a model of organization of the nucleus in which repressed genes are selectively recruited into centromeric domains.
Journal ArticleDOI
The transcriptional role of PML and the nuclear body
TL;DR: The current understanding of the biological functions of PML and the nuclear body is summarized, and a role for these intra-nuclear structures in the regulation of transcription is discussed.