Subgingival Microbial Profiles of Smokers with Periodontitis
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TLDR
The hypothesis that the subgingival biofilm is compositionally different in current and never-smokers is tested by using an open-ended molecular approach for bacterial identification, with significant differences in the prevalence and abundance of disease-associated and health-compatible organisms.Abstract:
The subgingival microbiome is largely uncultivated, and therefore, cultivation-based and targeted molecular approaches have limited value in examining the effect of smoking on this community. We tested the hypothesis that the subgingival biofilm is compositionally different in current and never-smokers by using an open-ended molecular approach for bacterial identification. Subgingival plaque from deep sites of current and never-smokers matched for disease was analyzed by 16S sequencing. Smokers demonstrated greater abundance of Parvimonas, Fusobacterium, Campylobacter, Bacteroides, and Treponema and lower levels of Veillonella, Neisseria, and Streptococcus. Several uncultivated Peptostreptococci, Parvimonas micra, Campylobacter gracilis, Treponema socranskii, Dialister pneumosintes, and Tannerella forsythia were elevated in this group, while Veillonella sp. oral clone B2, Neisseria sp. oral clone 2.24, Streptococcus sanguinis, and Capnocytophaga sp. clone AH015 were at lower levels. The microbial profile of smoking-associated periodontitis is distinct from that of non-smokers, with significant differences in the prevalence and abundance of disease-associated and health-compatible organisms.read more
Citations
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References
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Journal ArticleDOI
Microbial complexes in subgingival plaque
TL;DR: The purpose of the present investigation was to attempt to define communities using data from large numbers of plaque samples and different clustering and ordination techniques, which related strikingly to clinical measures of periodontal disease particularly pocket depth and bleeding on probing.
Journal ArticleDOI
Defining the Normal Bacterial Flora of the Oral Cavity
Jørn A. Aas,Jørn A. Aas,Bruce J. Paster,Bruce J. Paster,Lauren N. Stokes,Ingar Olsen,Floyd E. Dewhirst,Floyd E. Dewhirst +7 more
TL;DR: The purposes were to utilize culture-independent molecular techniques to extend the knowledge on the breadth of bacterial diversity in the healthy human oral cavity, including not-yet-cultivated bacteria species, and to determine the site and subject specificity of bacterial colonization.
Journal ArticleDOI
Bacterial Diversity in Human Subgingival Plaque
Bruce J. Paster,Bruce J. Paster,Susan K. Boches,Jamie L. Galvin,Rebecca E. Ericson,C. N. Lau,Valerie A. Levanos,Ashish Sahasrabudhe,Floyd E. Dewhirst,Floyd E. Dewhirst +9 more
TL;DR: The purpose of this study was to determine the bacterial diversity in the human subgingival plaque by using culture-independent molecular methods as part of an ongoing effort to obtain full 16S rRNA sequences for all cultivable and not-yet-cultivated species of human oral bacteria.
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