Subgingival Microbial Profiles of Smokers with Periodontitis
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TLDR
The hypothesis that the subgingival biofilm is compositionally different in current and never-smokers is tested by using an open-ended molecular approach for bacterial identification, with significant differences in the prevalence and abundance of disease-associated and health-compatible organisms.Abstract:
The subgingival microbiome is largely uncultivated, and therefore, cultivation-based and targeted molecular approaches have limited value in examining the effect of smoking on this community. We tested the hypothesis that the subgingival biofilm is compositionally different in current and never-smokers by using an open-ended molecular approach for bacterial identification. Subgingival plaque from deep sites of current and never-smokers matched for disease was analyzed by 16S sequencing. Smokers demonstrated greater abundance of Parvimonas, Fusobacterium, Campylobacter, Bacteroides, and Treponema and lower levels of Veillonella, Neisseria, and Streptococcus. Several uncultivated Peptostreptococci, Parvimonas micra, Campylobacter gracilis, Treponema socranskii, Dialister pneumosintes, and Tannerella forsythia were elevated in this group, while Veillonella sp. oral clone B2, Neisseria sp. oral clone 2.24, Streptococcus sanguinis, and Capnocytophaga sp. clone AH015 were at lower levels. The microbial profile of smoking-associated periodontitis is distinct from that of non-smokers, with significant differences in the prevalence and abundance of disease-associated and health-compatible organisms.read more
Citations
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The subgingival microbiome in health and periodontitis and its relationship with community biomass and inflammation
Loreto Abusleme,Amanda K. Dupuy,Nicolas Dutzan,Nora Silva,Joseph A. Burleson,Linda D. Strausbaugh,Jorge Gamonal,Patricia I. Diaz +7 more
TL;DR: This study provides a global-scale framework for the ecological events in subgingival communities that underline the development of periodontitis and elucidate the relationship between inflammation and the subgesival microbiome.
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Disordered microbial communities in the upper respiratory tract of cigarette smokers.
Emily S. Charlson,Jun Chen,Rebecca Custers-Allen,Kyle Bittinger,Hongzhe Li,Rohini Sinha,Jennifer Hwang,Frederic D. Bushman,Ronald G. Collman +8 more
TL;DR: Different regions of the human upper respiratory tract contain characteristic microbial communities that exhibit disordered patterns in cigarette smokers, both in individual components and global structure, which may contribute to the prevalence of respiratory tract complications in this population.
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References
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Periodontal microbial ecology.
TL;DR: This manuscript is a brief primer on microbial ecology, because, although the importance of microbial ecology in periodontal diseases is widely recognized, most of us do not know precisely what the term means.
Journal ArticleDOI
Smoking-Attributable Periodontitis in the United States: Findings From NHANES III
Scott L. Tomar,Samira Asma +1 more
TL;DR: It is concluded that smoking is a major risk factor forperiodontitis and may be responsible for more than half of periodontitis cases among adults in the United States.
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Identification of Candidate Periodontal Pathogens and Beneficial Species by Quantitative 16S Clonal Analysis
TL;DR: Chronic periodontitis is the result of a global perturbation of the oral bacterial ecology rather than a disease-site specific microbial shift, and more differences were found in the bacterial profile between subjects withperiodontitis and healthy subjects than between deep and shallow sites within the same subject.
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Cigarette smoking and the periodontal patient.
Georgia K. Johnson,Margaret Hill +1 more
TL;DR: The role of dental health professionals in tobacco cessation is discussed, including the use of the five A's: ask--identify tobacco users; advise--advise them to Quit; assess--evaluate the patient's readiness to quit; assist--offer assistance in cessation; and arrange--follow up on the patient’s cessation efforts.
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Relationship of cigarette smoking to the subgingival microbiota
TL;DR: The major difference between the subgingival microbiota in subjects with different smoking history was in the prevalence of species rather than counts or proportions.
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