Subgingival Microbial Profiles of Smokers with Periodontitis
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TLDR
The hypothesis that the subgingival biofilm is compositionally different in current and never-smokers is tested by using an open-ended molecular approach for bacterial identification, with significant differences in the prevalence and abundance of disease-associated and health-compatible organisms.Abstract:
The subgingival microbiome is largely uncultivated, and therefore, cultivation-based and targeted molecular approaches have limited value in examining the effect of smoking on this community. We tested the hypothesis that the subgingival biofilm is compositionally different in current and never-smokers by using an open-ended molecular approach for bacterial identification. Subgingival plaque from deep sites of current and never-smokers matched for disease was analyzed by 16S sequencing. Smokers demonstrated greater abundance of Parvimonas, Fusobacterium, Campylobacter, Bacteroides, and Treponema and lower levels of Veillonella, Neisseria, and Streptococcus. Several uncultivated Peptostreptococci, Parvimonas micra, Campylobacter gracilis, Treponema socranskii, Dialister pneumosintes, and Tannerella forsythia were elevated in this group, while Veillonella sp. oral clone B2, Neisseria sp. oral clone 2.24, Streptococcus sanguinis, and Capnocytophaga sp. clone AH015 were at lower levels. The microbial profile of smoking-associated periodontitis is distinct from that of non-smokers, with significant differences in the prevalence and abundance of disease-associated and health-compatible organisms.read more
Citations
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Journal ArticleDOI
Detection and quantification of periodontal pathogens in smokers and never-smokers with chronic periodontitis by real-time polymerase chain reaction.
Mariana Rocha Guglielmetti,Ecinele Francisca Rosa,Daniele Salami Lourenção,Gislene Inoue,E. F Gomes,Giorgio De Micheli,Fausto Medeiros Mendes,Rosario Dominguez Crespo Hirata,Mario Hiroyuki Hirata,Cláudio Mendes Pannuti +9 more
TL;DR: Smokers showed significantly greater amounts of P. gingivalis, A. actinomycetemcomitans, and T. forsythia than never-smokers and there was a significant association between smoking and the presence of A. actsinomyCetem comitans.
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Microcosm biofilms cultured from different oral niches in periodontitis patients
Fabian Cieplik,Egija Zaura,Bernd W. Brandt,Mark J. Buijs,Wolfgang Buchalla,Wim Crielaard,Marja L. Laine,Dong Mei Deng,R.A.M. Exterkate +8 more
TL;DR: Saliva, tongue scrapings or tonsil swabs may represent sufficient alternative inocula for growing microcosm biofilms resembling periodontitis-associated microbial communities in cases when sampling subgingival plaque is not possible.
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Host–Bacterial Interactions During Induction and Resolution of Experimental Gingivitis in Current Smokers
TL;DR: Both marginal and subgingival biofilms in smokers are characterized by early acquisition of pathogenic organisms, which elicit a sustained host response that persists even after removal of the bacterial challenge.
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Dysbiosis in the oral bacterial and fungal microbiome of HIV-infected subjects is associated with clinical and immunologic variables of HIV infection.
Pranab K. Mukherjee,Jyotsna Chandra,Mauricio Retuerto,Curtis Tatsuoka,Mahmoud A. Ghannoum,Grace A. McComsey +5 more
TL;DR: Dysbiosis of the oral microbiota is associated with clinical and immunologic variables in HIV-infected patients and was positively correlated with Facklamia but negatively with Enhydrobacter, and current alcohol use was negatively correlated with Geniculata.
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TL;DR: Kohl et al. this article presented Kohl's work in this article, which is based on the work of the University of Pittsburgh and Estados Unidos, USA.
References
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Journal ArticleDOI
Microbial complexes in subgingival plaque
TL;DR: The purpose of the present investigation was to attempt to define communities using data from large numbers of plaque samples and different clustering and ordination techniques, which related strikingly to clinical measures of periodontal disease particularly pocket depth and bleeding on probing.
Journal ArticleDOI
Defining the Normal Bacterial Flora of the Oral Cavity
Jørn A. Aas,Jørn A. Aas,Bruce J. Paster,Bruce J. Paster,Lauren N. Stokes,Ingar Olsen,Floyd E. Dewhirst,Floyd E. Dewhirst +7 more
TL;DR: The purposes were to utilize culture-independent molecular techniques to extend the knowledge on the breadth of bacterial diversity in the healthy human oral cavity, including not-yet-cultivated bacteria species, and to determine the site and subject specificity of bacterial colonization.
Journal ArticleDOI
Bacterial Diversity in Human Subgingival Plaque
Bruce J. Paster,Bruce J. Paster,Susan K. Boches,Jamie L. Galvin,Rebecca E. Ericson,C. N. Lau,Valerie A. Levanos,Ashish Sahasrabudhe,Floyd E. Dewhirst,Floyd E. Dewhirst +9 more
TL;DR: The purpose of this study was to determine the bacterial diversity in the human subgingival plaque by using culture-independent molecular methods as part of an ongoing effort to obtain full 16S rRNA sequences for all cultivable and not-yet-cultivated species of human oral bacteria.
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