Journal ArticleDOI
Summary of recombinant human serum albumin development
TLDR
Results from structural analysis suggest that purified rHSA possesses an identical conformation to plasma derived human albumin (pdHA) and no difference from pdHA has been observed in neo-antigenicity.About:
This article is published in Biologicals.The article was published on 2006-03-01. It has received 120 citations till now. The article focuses on the topics: Pichia pastoris & Human serum albumin.read more
Citations
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Journal ArticleDOI
Human serum albumin: from bench to bedside.
TL;DR: HSA is a valuable biomarker of many diseases, including cancer, rheumatoid arthritis, ischemia, post-menopausal obesity, severe acute graft-versus-host disease, and diseases that need monitoring of the glycemic control.
Journal ArticleDOI
The thiol pool in human plasma: the central contribution of albumin to redox processes.
TL;DR: A critical review of the plasma thiol pool is provided with a focus on human serum albumin, an important target for oxidants and electrophiles due to its reactivity with a wide variety of species and its relatively high concentration.
Journal ArticleDOI
Albumin and its application in drug delivery
TL;DR: The FcRn receptor has additional functionality, especially in relation to immunology, antigen presentation and delivery of proteins across mucosal membranes, consequently albumin-based fusions and conjugates may have a future role in oral and pulmonary-based vaccines and drug delivery.
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Human serum albumin (HSA) nanoparticles: Reproducibility of preparation process and kinetics of enzymatic degradation
Klaus Langer,Marion G. Anhorn,Isabel Steinhauser,Sebastian Dreis,D. Celebi,N. Schrickel,S. Faust,Vitali Vogel +7 more
TL;DR: It could be shown that the kinetics of particle degradation was dependent on the degree of particle stabilisation, which will influence drug release after cellular accumulation of HSA nanoparticles.
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Artificial oxygen carriers, hemoglobin vesicles and albumin-hemes, based on bioconjugate chemistry.
TL;DR: Manipulation of physicochemical parameters of HbV, such as O(2) binding affinity and suspension rheology, supports the use of HBV for versatile medical applications.
References
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Journal ArticleDOI
Crystal structure of human serum albumin at 2.5 A resolution.
TL;DR: A new triclinic crystal form of human serum albumin (HSA), derived either from pool plasma or from a Pichia pastoris expression system, was obtained from polyethylene glycol 4000 solution, and three-dimensional structures of pHSA and rHSA were determined.
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Recent advances in the expression of foreign genes in Pichia pastoris
TL;DR: The Pichia pastoris heterologous gene expression system has been utilized to produce attractive levels of a variety of intracellular and extracellular proteins of interest and improvements in understanding and application have improved its utility even further.
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Production of mouse epidermal growth factor in yeast: high-level secretion using Pichia pastoris strains containing multiple gene copies.
Jeffrey J. Clare,M A Romanos,F. B. Rayment,J E Rowedder,Marie-Louise Smith,M M Payne,Koti Tatachar Sreekrishna,C A Henwood +7 more
TL;DR: To increase the level of secreted mEGF, a synthetic secretion cassette encoding the alpha-factor prepro leader peptide from Saccharomyces cerevisiae fused to mouse epidermal growth factor was constructed and a method for rapidly screening large numbers of P. pastoris transformants for the presence of many copies of a foreign gene was developed.
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Molecular structure of the human albumin gene is revealed by nucleotide sequence within q11-22 of chromosome 4.
Phillip P. Minghetti,Duane E. Ruffner,Wun-Jing Kuang,O. E. Dennison,J. W. Hawkins,W. G. Beattie,Achilles Dugaiczyk +6 more
TL;DR: The human albumin gene spans 16,961 nucleotides from the putative "Cap" site to the first poly(A) addition site and appears to have been recently invaded by Alu repetitive sequences.
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Preliminary Crystallographic Studies of Four Crystal forms of Serum Albumin
TL;DR: Several crystal forms of serum albumin suitable for three-dimensional structure determination have been grown and two of the crystal forms produced from human and canine albumin include incorporated long-chain fatty acids.