Journal ArticleDOI
Survival of probiotic bacteria in the presence of food grade nanoparticles from chocolates: an in vitro and in vivo study.
Reads0
Chats0
TLDR
TiO2 from chocolates inhibits the growth and viability of probiotic bacteria in mice gut even at a concentration of 50–100 μg/day/mice, suggesting that TiO2 in chocolate discourages survival of probiotics in the human gut.Abstract:
The use of probiotics to treat gastrointestinal diseases such as diarrhea especially in children is becoming increasingly popular. Besides, the use of nanomaterials in food products is increasing rapidly especially in candies and chocolates. How these nanomaterials influence probiotic bacteria and their activity remains unexplored. Therefore, nanomaterials from commercial chocolate were purified and characterized by using SEM-EDS and XRD. The tested chocolate contained nano-TiO2 with an average size of ~ 40 nm. The influence of the extracted TiO2 on a commercial probiotic formulation usually used to treat diarrhea in children was studied. The probiotic formulation contained Bacillus coagulans, Enterococcus faecalis, and Enterococcus faecium as evident from 16S rRNA gene sequences and polyphasic characterization. Isolated bacteria exhibited known probiotic activities like biofilm formation, acid production, growth at 6% salt, and antibiotic resistance. TiO2 from chocolates inhibited the growth and activity of the probiotic formulation over a concentration range of 125-500μg/ml in vitro. Based on results, it is estimated that 20 g of such chocolate contains enough TiO2 to disturb the gut microbial community of children aged 2-8 years with a stomach capacity of ~ 0.5-0.9 l. The in vivo study on white albino mice shows the same response but with a higher dose. The results obtained by plate counts, MTT assay, live/dead staining, and qPCR suggest that TiO2 from chocolates inhibits the growth and viability of probiotic bacteria in mice gut even at a concentration of 50-100 μg/day/mice. Therefore, TiO2 in chocolate discourages survival of probiotic bacteria in the human gut.read more
Citations
More filters
Journal ArticleDOI
Identification of enterobacteriaceae
TL;DR: It is doubtful whether a general work of this calibre should contain even brief accounts and exiguous illustrations of operations, but the editors have produced a magnunm opus which for many years will be indispensable to those who practise obstetrics and gynaecology.
Journal ArticleDOI
An Updated Overview on Nanonutraceuticals: Focus on Nanoprebiotics and Nanoprobiotics
Alessandra Durazzo,Amirhossein Nazhand,Massimo Lucarini,Atanas G. Atanasov,Eliana B. Souto,Eliana B. Souto,Ettore Novellino,Raffaele Capasso,Antonello Santini +8 more
TL;DR: The main features of prebiotics and probiotics are highlighted, together with the discussion of emerging applications of nanotechnologies in their formulation, and current research strategies are discussed, in particular the promising use of nanofibers for the delivery of probiotics.
Journal ArticleDOI
Interplay between engineered nanomaterials and microbiota
TL;DR: The relative abundances of bacterial phyla indicate that ENMs have a potential to modulate intestinal microbiota and induce harmful or beneficial effects in the host, and future perspectives include identification of the factors driving ENM-caused dysbiosis and the ENM impacts on microbiota with deviated composition.
Journal ArticleDOI
Long-term exposure to titanium dioxide nanoparticles promotes diet-induced obesity through exacerbating intestinal mucus layer damage and microbiota dysbiosis
TL;DR: The results stated the alarming role of TiO 2 -NPs in the HFD-driven obesity and emphasized the reevaluating the health impacts of nanoparticles commonly used in daily life, particularly, in susceptible population.
Journal ArticleDOI
Nanotechnology for Targeted Detection and Removal of Bacteria: Opportunities and Challenges.
Mohammad Javad Hajipour,Amir Ata Saei,Edward D. Walker,Brian Conley,Yadollah Omidi,Ki-Bum Lee,Morteza Mahmoudi +6 more
TL;DR: A wide range of developed nanotechnology-based approaches for bacterial detection and removal together with biofilm eradication are summarized in this paper, where the challenging effects of nanotechnologies on beneficial bacteria in the human body and environment and the mechanisms of bacterial resistance to nanotherapeutics are also reviewed.
References
More filters
Journal ArticleDOI
Basic Local Alignment Search Tool
TL;DR: A new approach to rapid sequence comparison, basic local alignment search tool (BLAST), directly approximates alignments that optimize a measure of local similarity, the maximal segment pair (MSP) score.
Journal ArticleDOI
The neighbor-joining method: a new method for reconstructing phylogenetic trees.
Naruya Saitou,Masatoshi Nei +1 more
TL;DR: The neighbor-joining method and Sattath and Tversky's method are shown to be generally better than the other methods for reconstructing phylogenetic trees from evolutionary distance data.
Journal ArticleDOI
Confidence limits on phylogenies: an approach using the bootstrap.
TL;DR: The recently‐developed statistical method known as the “bootstrap” can be used to place confidence intervals on phylogenies and shows significant evidence for a group if it is defined by three or more characters.
Journal ArticleDOI
Antibiotic susceptibility testing by a standardized single disk method.
TL;DR: Recommendations of the National Committee for Clinical Laboratory Standards continue to be based on this publication; the “Kirby-Bauer” method is, among the many disk methods used in other countries, still the one that has been researched most thoroughly and updated continuously.
Journal ArticleDOI
The impact of the gut microbiota on human health: an integrative view.
TL;DR: It is suggested that a holistic approach to studying the microbiota that goes beyond characterization of community composition and encompasses dynamic interactions between all components of the microbiota and host tissue over time will be crucial for building predictive models for diagnosis and treatment of diseases linked to imbalances in the microbiota.