Journal ArticleDOI
Synergistic activity profile of an antimicrobial peptide against multidrug‐resistant and extensively drug‐resistant strains of Gram‐negative bacterial pathogens
Simona Pollini,Simona Pollini,Jlenia Brunetti,Samanta Sennati,Gian Maria Rossolini,Luisa Bracci,Alessandro Pini,Chiara Falciani +7 more
TLDR
SET‐M33 is a novel antimicrobial peptide that has demonstrated in vitro and in vivo antimicrobial activity against Gram‐negative bacteria and has shown interesting features in preclinical evaluations.Abstract:
Infection sustained by multidrug-resistant and extensively drug-resistant bacterial pathogens is often untreatable with the standard of care antibiotics, and the combination of anti-infective compounds often represents the only therapeutic strategy to face this major clinical treat. SET-M33 is a novel antimicrobial peptide (AMP) that has demonstrated in vitro and in vivo antimicrobial activity against Gram-negative bacteria and has shown interesting features in preclinical evaluations. Particularly, it showed efficacy against a number of multidrug-resistant and extensively drug-resistant clinical strains of Gram-negative pathogens, in in vitro and in vivo assessments. Here, we explored the potential synergistic activity of SET-M33 in combination with different standard of care antibiotics by the checkerboard method against a panel of six strains of Gram-negative pathogens including multidrug-resistant and extensively drug-resistant Klebsiella pneumoniae, Pseudomonas aeruginosa, and Acinetobacter baumannii. SET-M33 showed synergistic activity with antibiotics of different families against these clinically relevant strains. A synergistic effect was observed for SET-M33 in combination with rifampin, meropenem, aztreonam, and tobramycin mostly on K. pneumoniae and A. baumannii strains, while the SET-M33 plus ciprofloxacin combination was additive with all tested strains. Synergy was not apparently linked to the bacterial species or phenotype but was rather strain-specific, highlighting the need for individual strain testing for synergistic antimicrobial combinations. These findings extend current knowledge on synergistic activity of AMPs in combination with conventional agents and support the potential role of SET-M33 as a novel therapeutic agent against antibiotic-resistant Gram-negative pathogens. Copyright © 2017 European Peptide Society and John Wiley & Sons, Ltd.read more
Citations
More filters
Journal ArticleDOI
Application of Antimicrobial Peptides of the Innate Immune System in Combination With Conventional Antibiotics-A Novel Way to Combat Antibiotic Resistance?
M. S. Zharkova,D. S. Orlov,Olga Yu. Golubeva,Oleg B. Chakchir,Igor E. Eliseev,Tatyana M. Grinchuk,O. V. Shamova +6 more
TL;DR: Examination of a combined action of natural AMPs with different structure and mode of action with varied antibiotic agents found that synergy in antibacterial action mainly occurs between highly membrane-active AMPs and antibiotics with intracellular targets, suggesting bioavailability increase as the main model of such interaction.
Journal ArticleDOI
Antimicrobial Susceptibility Testing of Antimicrobial Peptides to Better Predict Efficacy.
Derry K. Mercer,Marcelo Der Torrossian Torres,Searle S. Duay,Emma Lovie,Laura Simpson,Maren von Köckritz-Blickwede,Cesar de la Fuente-Nunez,Deborah O'neil,Alfredo M. Angeles-Boza +8 more
TL;DR: What is believed to be the key considerations for AST of AMP are described and it is hoped that this information can better guide the preclinical development of AMp toward becoming a new generation of urgently needed antimicrobials.
Journal ArticleDOI
Antimicrobial Peptide-Loaded Nanoparticles as Inhalation Therapy for Pseudomonas aeruginosa Infections
Chiara Falciani,Fabrizia Zevolini,Jlenia Brunetti,Giulia Riolo,Raquel Gracia,Marco Marradi,Iraida Loinaz,Christina Ziemann,Unai Cossío,Jordi Llop,Luisa Bracci,Alessandro Pini +11 more
TL;DR: The encapsulation of the antimicrobial peptide in dextran nanoparticles markedly improved lung residence time of the peptide administered via aerosol, which has to be considered among the aims of the development of a new therapeutic option for patients suffering recurrent infections, that will benefit from high local doses of persistent antimicrobials.
Journal ArticleDOI
Combinatory Therapy Antimicrobial Peptide-Antibiotic to Minimize the Ongoing Rise of Resistance
TL;DR: This article focuses on the main aspects of the combinatory therapy antimicrobial peptide (AMP)-antibiotic to treat infectious diseases.
Journal ArticleDOI
Highly Synergistic Effects of Melittin with Conventional Antibiotics Against Multidrug-Resistant Isolates of Acinetobacter baumannii and Pseudomonas aeruginosa
Reza Akbari,Mojdeh Hakemi-Vala,Fatemeh Pashaie,Parvaneh Bevalian,Ali Hashemi,Kamran Pooshang Bagheri +5 more
TL;DR: The synergism of melittin at its nontoxic dose with doripenem and ceftazidime could be of great therapeutic value as a topical drug against burn infections caused by MDR bacteria.
References
More filters
Journal ArticleDOI
Multidrug-resistant, extensively drug-resistant and pandrug-resistant bacteria: an international expert proposal for interim standard definitions for acquired resistance
A-P Magiorakos,Arjun Srinivasan,R B Carey,Yehuda Carmeli,Matthew E. Falagas,Matthew E. Falagas,Christian G. Giske,Stéphan Juergen Harbarth,Janet F. Hindler,Gunnar Kahlmeter,Barbro Olsson-Liljequist,David L. Paterson,Louis B. Rice,John Stelling,Marc Struelens,Alkiviadis Vatopoulos,J T Weber,Dominique L Monnet +17 more
TL;DR: A group of international experts came together through a joint initiative by the European Centre for Disease Prevention and Control and the Centers for Disease Control and Prevention, to create a standardized international terminology with which to describe acquired resistance profiles in Staphylococcus aureus, Enterococcus spp.
Journal ArticleDOI
Staphylococcus aureus; drug-fastness studies with penicillin and sulfactin.
TL;DR: A strain of staphylococci in becoming resistant to penicillin did not show an increase in its resistance to sulfactin and the increase in resistance developed by a strain of E. coli to streptomycin was intermediate.
Journal ArticleDOI
Multiresistant Gram-negative bacteria: the role of high-risk clones in the dissemination of antibiotic resistance
TL;DR: 'high-risk clones' play a major role in the spread of resistance, with the risk lying in their tenacity--deriving from poorly understood survival traits--and a flexible ability to accumulate and switch resistance, rather than to constant resistance batteries.
Journal ArticleDOI
Update on the antibiotic resistance crisis.
TL;DR: Strains with resistance to multiple antibiotic classes have emerged among major Gram-positive and Gram-negative species including Staphylococcus aureus, Enterococcus spp.
Journal ArticleDOI
Antibiotic adjuvants: multicomponent anti-infective strategies
Lindsay Kalan,Gerard D. Wright +1 more
TL;DR: The current situation and potential drug combinations that may increase the potency of antibiotics in the future are reviewed and a rationale for these multicomponent strategies is provided.
Related Papers (5)
Optimized arylomycins are a new class of Gram-negative antibiotics.
Peter A. S. Smith,Michael F. T. Koehler,Hany S. Girgis,Donghong Yan,Yongsheng Chen,Yuan Chen,James J. Crawford,Matthew R. Durk,Robert I. Higuchi,Jing Kang,Jeremy Murray,Prasuna Paraselli,Summer Park,Wilson Phung,John G. Quinn,Tucker C. Roberts,Lionel Rouge,Jacob Schwarz,Elizabeth Skippington,John S. Wai,Min Xu,Zhiyong Yu,Hua Zhang,Man-Wah Tan,Christopher E. Heise +24 more