Synthetic scale-up of a novel fluorescent probe and its biological evaluation for surface detection of Staphylococcus aureus.
TLDR
The LGX fluorometric test for enzymatic MRSA/MSSA detection is reported on, highlighting the reasons rhodamines have been overlooked and also strategies to improve the synthesis of rhodamine-peptide conjugates.About:
This article is published in Molecular and Cellular Probes.The article was published on 2017-12-01 and is currently open access. It has received 2 citations till now.read more
Citations
More filters
Journal ArticleDOI
Ratiometric fluorescent probes for selective and sensitive visualization of bacterial microenvironment protease activity.
Qinghua Wang,Xiang Wang,Ying Sun,Xiaoxiao Yang,Xiaoxiao Yang,Leilei Zhang,Qingyang Zhang,Zhi-Qiang Hu,Hai-Yu Hu +8 more
TL;DR: The extracellular SspA protease of the human pathogen S. aureus is essential for evading the host immune defence system and two novel FRET probes for selective real-time ratiometric imaging of SSPA activity in live S.Aureus cells are reported.
Journal ArticleDOI
Synthesis and Spectral Properties of Novel Singapore Green Analogues for Protease Detection.
Luke Bywaters,Adam Le Gresley +1 more
TL;DR: Proof of concept that the use of Singapore Greens for protease probe generation is feasible via demonstration of proteolytic cleavage of one of the acylated analogues is obtained.
References
More filters
Journal ArticleDOI
Rhodamine-based compounds as fluorogenic substrates for serine proteinases.
TL;DR: A new fluorogenic substrate for serine proteinases, bis(N-benzyloxycarbonyl-L-argininamido)Rhodamine [(Cbz-Arg-NH)2-R Rhodamine], was synthesized, purified and chemically and enzymically characterized.
Journal ArticleDOI
Impact of rapid screening tests on acquisition of meticillin resistant Staphylococcus aureus: cluster randomised crossover trial
Dakshika Jeyaratnam,Christopher J. M. Whitty,Katie M. Phillips,Dongmei Liu,Christina Orezzi,Uchechukwu Ajoku,Gary French +6 more
TL;DR: No evidence was found of a significant reduction in MRSA acquisition and on these data it is unlikely that the increased costs of rapid tests can be justified compared with alternative control measures against MRSA.
Journal ArticleDOI
New class of sensitive and selective fluorogenic substrates for serine proteinases. Amino acid and dipeptide derivatives of rhodamine.
TL;DR: Comparison of the kinetic constants for hydrolysis of the dipeptide substrates with that of the single amino acid derivative, (Cbz-Arg-NH)2-Rhodamine, indicates that selection of the proper amino acid residue in the P2 position can effect large increases in substrate specificity.
Journal ArticleDOI
Design and synthesis of rhodamine 110 derivative and caspase-3 substrate for enzyme and cell-based fluorescent assay.
TL;DR: The tetrapeptide substrate N-Ac-DEVD-N'-octyloxycarbonyl-R110 was prepared and shown to be efficiently cleaved by human recombinant caspase-3 and by apoptotic HL-60 cells, which should prove useful in cell-based assays for apoptosis inducers and inhibitors.
Journal ArticleDOI
Samarium(II) di-iodide induced reductive coupling of α,β-unsaturated esters with carbonyl compounds leading to a facile synthesis of γ-lactone
TL;DR: In this article, samarium(II) di-iodide is used for the reductive coupling of α,β-unsaturated esters with carbonyl compounds, whereby substituted γ-lactones can easily be prepared in good to excellent yields under very mild conditions.