Synthetic scale-up of a novel fluorescent probe and its biological evaluation for surface detection of Staphylococcus aureus.
TLDR
The LGX fluorometric test for enzymatic MRSA/MSSA detection is reported on, highlighting the reasons rhodamines have been overlooked and also strategies to improve the synthesis of rhodamine-peptide conjugates.About:
This article is published in Molecular and Cellular Probes.The article was published on 2017-12-01 and is currently open access. It has received 2 citations till now.read more
Citations
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Ratiometric fluorescent probes for selective and sensitive visualization of bacterial microenvironment protease activity.
Qinghua Wang,Xiang Wang,Ying Sun,Xiaoxiao Yang,Xiaoxiao Yang,Leilei Zhang,Qingyang Zhang,Zhi-Qiang Hu,Hai-Yu Hu +8 more
TL;DR: The extracellular SspA protease of the human pathogen S. aureus is essential for evading the host immune defence system and two novel FRET probes for selective real-time ratiometric imaging of SSPA activity in live S.Aureus cells are reported.
Journal ArticleDOI
Synthesis and Spectral Properties of Novel Singapore Green Analogues for Protease Detection.
Luke Bywaters,Adam Le Gresley +1 more
TL;DR: Proof of concept that the use of Singapore Greens for protease probe generation is feasible via demonstration of proteolytic cleavage of one of the acylated analogues is obtained.
References
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Preparation of Samarium(II) Iodide: Quantitative Evaluation of the Effect of Water, Oxygen, and Peroxide Content, Preparative Methods, and the Activation of Samarium Metal
TL;DR: The studies confirm that SmI(2) is a user-friendly reagent and suggest that the quality of samarium metal is an important factor and that the use of low quality metal is the main cause of failed preparations of the reagent.
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The reaction of samarium(III) iodide with samarium metal in tetrahydrofuran. A new method for the preparation of samarium(II) iodide
Tsuneo Imamoto,Mitsumasa Ono +1 more
TL;DR: Samarium(III) iodide reacts with samarium metal in tetrahydrofuran under mild conditions to afford samarium(II)-I iodide in almost quantitative yield as discussed by the authors.
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A Homobifunctional Rhodamine for Labeling Proteins with Defined Orientations of a Fluorophore
TL;DR: The synthesis and characterization of a bifunctional rhodamine dye bearing 2-(iodoacetamido)ethyl substituents on the 3'- and 6'-nitrogen atoms is described, including a remarkably mild dehalogenation of an aromatic haloacetamide in the presence of NaI and camphorsulfonic acid.
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New latent fluorophore for DT diaphorase.
Sheng-Tung Huang,Yuh-Ling Lin +1 more
TL;DR: The design and synthesis of a novel latent fluorophore 3 for DT diaphorase based on the trimethyl lock effect and characterization of its enzymatic kinetics are described.
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Total synthesis of (+)-chloranthalactone F
Shan Qian,Gang Zhao +1 more
TL;DR: Sequential biomimetic elaborations, featured by CrO(3)-mediated oxidative lactonization, and DDQ-involved oxidative enol-lactonized, ensured the concise total synthesis of (+)-chloranthalactone F.