Synthetic scale-up of a novel fluorescent probe and its biological evaluation for surface detection of Staphylococcus aureus.
TLDR
The LGX fluorometric test for enzymatic MRSA/MSSA detection is reported on, highlighting the reasons rhodamines have been overlooked and also strategies to improve the synthesis of rhodamine-peptide conjugates.About:
This article is published in Molecular and Cellular Probes.The article was published on 2017-12-01 and is currently open access. It has received 2 citations till now.read more
Citations
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Ratiometric fluorescent probes for selective and sensitive visualization of bacterial microenvironment protease activity.
Qinghua Wang,Xiang Wang,Ying Sun,Xiaoxiao Yang,Xiaoxiao Yang,Leilei Zhang,Qingyang Zhang,Zhi-Qiang Hu,Hai-Yu Hu +8 more
TL;DR: The extracellular SspA protease of the human pathogen S. aureus is essential for evading the host immune defence system and two novel FRET probes for selective real-time ratiometric imaging of SSPA activity in live S.Aureus cells are reported.
Journal ArticleDOI
Synthesis and Spectral Properties of Novel Singapore Green Analogues for Protease Detection.
Luke Bywaters,Adam Le Gresley +1 more
TL;DR: Proof of concept that the use of Singapore Greens for protease probe generation is feasible via demonstration of proteolytic cleavage of one of the acylated analogues is obtained.
References
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A new method for the hydroxymethylation of carbonyl compounds
TL;DR: In this article, it was shown that benzyl chloromethyl ether reacts with carbonyl compounds in the presence of SmI2 to afford alcohols, which are subsequently subjected to hydrogenolysis to yield diols.
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Impact of rapid screening tests on acquisition of meticillin resistant Staphylococcus aureus: cluster randomised crossover trial
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The National One Week Prevalence Audit of Universal Meticillin-Resistant Staphylococcus aureus (MRSA) Admission Screening 2012
Christopher Fuller,Julie V. Robotham,Joanne Savage,Susan Hopkins,Susan Hopkins,Sarah R Deeny,S. Stone,S. Stone,Barry Cookson,Barry Cookson +9 more
TL;DR: A national audit to review implementation, impact on patient management, admission prevalence and extra yield of MRSA identified compared to “high-risk” specialty or “checklist-activated” screening (CLAS) of patients with MRSA risk factors found implementation was poor.
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Dialkylhydrazides for directed orthometalations
TL;DR: Dimethylhydrazides are shown to be an excellent substrate for the directed orthometalation reaction as mentioned in this paper, and they can be easily removed by treatment with H 5 IO 6 or CuCl 2 to give the carboxylic acid under very mild conditions, in contrast to other amides that generally require harshly acidic conditions to achieve hydrolysis.
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Substrate optimization for monitoring cathepsin C activity in live cells.
Jun Li,H. Michael Petrassi,Christine Tumanut,Brian T. Masick,Christopher Trussell,Jennifer L. Harris +5 more
TL;DR: A series of peptidic fluorogenic substrates were synthesized to develop a flow cytometry assay (FACS) to monitor the proteolytic activity of cathepsin C in live cells and (NH(2)-aminobutyric-homophenylalanine)(2)-rhodamine demonstrated the best reactivity and selectivity profile in the FACS assay.