Journal ArticleDOI
Ratiometric fluorescent probes for selective and sensitive visualization of bacterial microenvironment protease activity.
Qinghua Wang,Xiang Wang,Ying Sun,Xiaoxiao Yang,Xiaoxiao Yang,Leilei Zhang,Qingyang Zhang,Zhi-Qiang Hu,Hai-Yu Hu +8 more
TLDR
The extracellular SspA protease of the human pathogen S. aureus is essential for evading the host immune defence system and two novel FRET probes for selective real-time ratiometric imaging of SSPA activity in live S.Aureus cells are reported.About:
This article is published in Chemical Communications.The article was published on 2019-04-25. It has received 8 citations till now.read more
Citations
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Enzyme-Activated Fluorogenic Probes for Live-Cell and in Vivo Imaging
Wen Chyan,Ronald T. Raines +1 more
TL;DR: Fluorogenic probes, small-molecule sensors that unmask brilliant fluorescence upon exposure to specific stimuli, are powerful tools for chemical biology and have been widely used for biological imaging as mentioned in this paper.
Journal ArticleDOI
Effective detection of bacteria using metal nanoclusters
TL;DR: This review summarizes multiple strategies that utilize nanoclusters for detection of pathogenic bacteria and their distinct optical and catalytic properties enables them as efficient biosensors.
Journal ArticleDOI
Small-molecule fluorescent probes: big future for specific bacterial labeling and infection detection
TL;DR: In this paper , the authors focus on the recent advances of small-molecule probes developed for fluorescent imaging of bacteria and infection, which covers the probe design, responsive mechanisms and representative applications.
Journal ArticleDOI
Enzyme-targeted fluorescent small-molecule probes for bacterial imaging
TL;DR: Examples from the recent literature are highlighted that showcase the utility of small-molecule probes for the fluorescence imaging of bacterial cells, including electrophilic, metabolic, and enzyme-activated probes.
Journal ArticleDOI
From Differential Stains to Next Generation Physiology: Chemical Probes to Visualize Bacterial Cell Structure and Physiology.
TL;DR: A variety of different probe types are introduced including fluorogenic substrates and activity- based probes that visualize metabolic and specific enzyme activities, metabolic labeling strategies to visualize structural features of bacterial cells, antibiotic-based probes as well as fluorescent conjugates to probe biomolecular uptake pathways are discussed.
References
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Journal ArticleDOI
FRET-based small-molecule fluorescent probes: rational design and bioimaging applications.
TL;DR: The most recent progress is emphasized on the development of FRET probes by spectral overlap integral, in particular by changing the molar absorption coefficient of the donor dyes such as rhodamine dyes, which undergo unique changes in the absorption profiles during the ring-opening and -closing processes.
Journal ArticleDOI
Quantitation of Transcription and Clonal Selection of Single Living Cells with β-Lactamase as Reporter
Gregor Zlokarnik,Paul A. Negulescu,Thomas E. Knapp,Lora Mere,Neal Burres,Luxin Feng,Michael A. Whitney,Klaus Roemer,Roger Y. Tsien +8 more
TL;DR: The robust change in emission ratio reveals quantitative heterogeneity in real-time gene expression, enables clonal selection by flow cytometry, and forms a basis for high-throughput screening of pharmaceutical candidate drugs in living mammalian cells.
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Energy transfer cassettes based on organic fluorophores: construction and applications in ratiometric sensing
TL;DR: This tutorial review presented some recent developments in the construction and applications of cassettes based on resonance energy transfer between fluorescent dyes in the visible and infrared region, and focused on the contributions of different connections between the energy donor and acceptor according to the " through-space" and "through-bond" methods.
Journal ArticleDOI
Methicillin-resistant Staphylococcus aureus.
Andie S Lee,Andie S Lee,Hermínia de Lencastre,Hermínia de Lencastre,Javier Garau,Jan Kluytmans,Surbhi Malhotra-Kumar,Andreas Peschel,Stéphan Juergen Harbarth +8 more
TL;DR: The success of MRSA is a consequence of the extensive arsenal of virulence factors produced by S. aureus combined with β-lactam resistance and, for most clones, resistance to other antibiotic classes as discussed by the authors.
Journal ArticleDOI
Novel antibody–antibiotic conjugate eliminates intracellular S. aureus
Sophie M. Lehar,Thomas H. Pillow,Min Xu,Leanna Staben,Kimberly Kajihara,Richard Vandlen,Laura DePalatis,Helga Raab,Wouter L. W. Hazenbos,J. Hiroshi Morisaki,Janice Kim,Summer Park,Martine Darwish,Byoung-Chul Lee,Hilda Hernandez,Kelly M. Loyet,Patrick J. Lupardus,Rina Fong,Donghong Yan,Cecile Chalouni,Elizabeth Luis,Yana Khalfin,Emile Plise,Jonathan Cheong,Joseph P. Lyssikatos,Magnus Strandh,Klaus Koefoed,Peter S. Andersen,John A. Flygare,Man-Wah Tan,Eric J. Brown,Sanjeev Mariathasan +31 more
TL;DR: The antibody–antibiotic conjugate is superior to vancomycin for treatment of bacteraemia and provides direct evidence that intracellular S. aureus represents an important component of invasive infections.