Telomerase: an RNP enzyme synthesizes DNA.
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TLDR
The underlying conservation of a core set of structural features of telomerase RNAs important for the fundamental polymerase activity of telomersase are described, which include a pseudoknot-plus-template domain and at least one other RNA structural motif separate from the template-containing domain.Abstract:
SUMMARY Telomerase is a eukaryotic ribonucleoprotein (RNP) whose specialized reverse transcriptase action performs de novo synthesis of one strand of telomeric DNA. The resulting telomerase-mediated elongation of telomeres, which are the protective end-caps for eukaryotic chromosomes, counterbalances the inevitable attrition from incomplete DNA replication and nucleaseaction.Thetelomerasestrategytomaintaintelomeresisdeeplyconservedamongeukaryotes, yet the RNA component of telomerase, which carries the built-in template for telomeric DNA repeat synthesis, has evolutionarily diverse size and sequence. Telomerase shows adistributionoflaborbetweenRNAandproteininaspectsofthepolymerizationreaction.This article first describesthe underlying conservation of acore set of structural features of telomerase RNAs important for the fundamental polymerase activity of telomerase. These include a pseudoknot-plus-template domain and at least one other RNA structural motif separate from the template-containing domain. The principles driving the diversity of telomerase RNAs are then explored. Much of the diversification of telomerase RNAs has come from apparent gain-of-function elaborations, through inferred evolutionaryacquisitions of various RNA motifs used for telomerase RNP biogenesis, cellular trafficking of enzyme components, and regulation of telomerase action at telomeres. Telomerase offers broadly applicable insights into the interplay of protein and RNA functions in the context of an RNP enzyme.read more
Citations
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Long noncoding RNAs: past, present, and future.
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The telomere syndromes
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Telomeres in cancer: tumour suppression and genome instability
John Maciejowski,Titia de Lange +1 more
TL;DR: Current data, reviewed here, provide new evidence for the telomere tumour suppressor pathway and has revealed that telomeres crisis can induce numerous cancer-relevant changes, including chromothripsis, kataegis and tetraploidization.
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Human telomerase: biogenesis, trafficking, recruitment, and activation
Jens C. Schmidt,Thomas R. Cech +1 more
TL;DR: This review covers human telomerase biogenesis, trafficking, and activation, comparing key aspects with the analogous events in other species.
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Telomeric G-quadruplexes are a substrate and site of localization for human telomerase
Aaron L. Moye,Karina C. Porter,Scott B. Cohen,Tram Phan,Katherine G. Zyner,Natsuki Sasaki,George O. Lovrecz,Jennifer L. Beck,Tracy M. Bryan +8 more
TL;DR: It is demonstrated that human telomerase is able to extend parallel, intermolecular conformations in vitro, and this implies that parallel telomeric G-quadruplexes may play an important biological role.
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