The extracellular matrix protects Pseudomonas aeruginosa biofilms by limiting the penetration of tobramycin.
Boo Shan Tseng,Wei Zhang,Joe J. Harrison,Tam P. Quach,Jisun L. Song,Jon Penterman,Jon Penterman,Pradeep K. Singh,David L. Chopp,Aaron I. Packman,Matthew R. Parsek +10 more
TLDR
It is proposed that tobramycin sequestration at the biofilm periphery is an important mechanism in protecting metabolically active cells that lie just below the zone of sequestration.Abstract:
Biofilm cells are less susceptible to antimicrobials than their planktonic counterparts. While this phenomenon is multifactorial, the ability of the matrix to reduce antibiotic penetration into the biofilm is thought to be of limited importance studies suggest that antibiotics move fairly rapidly through biofilms. In this study, we monitored the transport of two clinically relevant antibiotics, tobramycin and ciprofloxacin, into non-mucoid Pseudomonas aeruginosa biofilms. To our surprise, we found that the positively charged antibiotic tobramycin is sequestered to the biofilm periphery, while the neutral antibiotic ciprofloxacin readily penetrated. We provide evidence that tobramycin in the biofilm periphery both stimulated a localized stress response and killed bacteria in these regions but not in the underlying biofilm. Although it is unclear which matrix component binds tobramycin, its penetration was increased by the addition of cations in a dose-dependent manner, which led to increased biofilm death. These data suggest that ionic interactions of tobramycin with the biofilm matrix limit its penetration. We propose that tobramycin sequestration at the biofilm periphery is an important mechanism in protecting metabolically active cells that lie just below the zone of sequestration.read more
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Alginate oligosaccharides enhance diffusion and activity of colistin in a mucin-rich environment
Joana Stokniene,Mathieu Varache,Phil D. Rye,Katja E. Hill,David Thomas,Elaine Lesley Ferguson +5 more
TL;DR: In this article , a 2-compartment Transwell model was developed to study impaired diffusion of the antibiotic colistin across an artificial sputum (AS) matrix/medium and to quantify its antimicrobial activity against Pseudomonas aeruginosa NH57388A biofilms (alone and in combination with mucolytic therapy).
Journal ArticleDOI
Efficacy of a ciprofloxacin/amikacin combination against planktonic and biofilm cultures of susceptible and low-level resistant Pseudomonas aeruginosa.
Anaïs Soares,Kevin Alexandre,Fabien Lamoureux,Ludovic Lemée,François Caron,Martine Pestel-Caron,Manuel Etienne +6 more
TL;DR: The ciprofloxacin/amikacin combination prevented the emergence of resistant mutants in low-level resistant strains in planktonic cultures and biofilm persister cells were not eradicated, either with monotherapy or with the combination.
Journal ArticleDOI
MacAB-TolC Contributes to the Development of Acinetobacter baumannii Biofilm at the Solid–Liquid Interface
Brandon Robin,Marion Nicol,Hung Thai Le,Ali Tahrioui,Annick Schaumann,Jean-Baptiste Vuillemenot,D. Vergoz,Olivier Lesouhaitier,Thierry Jouenne,Julie Hardouin,Anaïs Potron,Valérie Perrot,Emmanuelle Dé +12 more
TL;DR: To unravel critical determinants of this sessile lifestyle, the proteomic profiles of two A. baumannii strains grown in planktonic stationary phase or in mature solid–liquid (S-L) biofilm were compared and this efflux pump appeared to be involved in the envelope stress response occurring in mature biofilm.
Journal ArticleDOI
Liquid crystal nanoparticle platform for increased efficacy of cationic antimicrobials against biofilm infections.
Chelsea R. Thorn,Zlatko Kopecki,Anthony Wignall,A. Jude Kral,Clive A. Prestidge,Nicky Thomas +5 more
TL;DR: In this article , liquid crystal nanoparticles (LCNPs) were used to improve the efficacy of tobramycin in biofilm-related infections by increasing the penetration of antibiotics across the biofilm matrix.
Journal ArticleDOI
Neutralization of ionic interactions by dextran-based single-chain nanoparticles improves tobramycin diffusion into a mature biofilm
Núria Blanco-Cabra,Julie Movellan,Marco Marradi,Raquel Gracia,Cristian Salvador,Damien Dupin,Iraida Loinaz,Eduard Torrents +7 more
TL;DR: In this article , the authors combine the charge neutralization of tobramycin provided by dextran-based single-chain polymer nanoparticles (SCPNs) together with DNase I to break the biofilm matrix.
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