The extracellular matrix protects Pseudomonas aeruginosa biofilms by limiting the penetration of tobramycin.
Boo Shan Tseng,Wei Zhang,Joe J. Harrison,Tam P. Quach,Jisun L. Song,Jon Penterman,Jon Penterman,Pradeep K. Singh,David L. Chopp,Aaron I. Packman,Matthew R. Parsek +10 more
TLDR
It is proposed that tobramycin sequestration at the biofilm periphery is an important mechanism in protecting metabolically active cells that lie just below the zone of sequestration.Abstract:
Biofilm cells are less susceptible to antimicrobials than their planktonic counterparts. While this phenomenon is multifactorial, the ability of the matrix to reduce antibiotic penetration into the biofilm is thought to be of limited importance studies suggest that antibiotics move fairly rapidly through biofilms. In this study, we monitored the transport of two clinically relevant antibiotics, tobramycin and ciprofloxacin, into non-mucoid Pseudomonas aeruginosa biofilms. To our surprise, we found that the positively charged antibiotic tobramycin is sequestered to the biofilm periphery, while the neutral antibiotic ciprofloxacin readily penetrated. We provide evidence that tobramycin in the biofilm periphery both stimulated a localized stress response and killed bacteria in these regions but not in the underlying biofilm. Although it is unclear which matrix component binds tobramycin, its penetration was increased by the addition of cations in a dose-dependent manner, which led to increased biofilm death. These data suggest that ionic interactions of tobramycin with the biofilm matrix limit its penetration. We propose that tobramycin sequestration at the biofilm periphery is an important mechanism in protecting metabolically active cells that lie just below the zone of sequestration.read more
Citations
More filters
Journal ArticleDOI
Molecular mechanisms of biofilm-based antibiotic resistance and tolerance in pathogenic bacteria
Clayton W. Hall,Thien-Fah Mah +1 more
TL;DR: This review summarises both historical and recent scientific data in support of the known biofilm resistance and tolerance mechanisms and suggestions for future work in the field are provided.
Journal ArticleDOI
Biofilm-Related Infections: Bridging the Gap between Clinical Management and Fundamental Aspects of Recalcitrance toward Antibiotics
TL;DR: This review presents the current understanding of the molecular mechanisms of biofilm recalcitrance toward antibiotics and describes how recent progress has improved the capacity to design original and efficient strategies to prevent or eradicate biofilm-related infections.
Journal ArticleDOI
Bacterial biofilm formation on implantable devices and approaches to its treatment and prevention
TL;DR: A brief overview of concepts of bacterial biofilm formation, current state-of-the-art therapeutic approaches for preventing and treating biofilms, and the prevalence of such infections on medical devices is reviewed.
Journal ArticleDOI
Nanomaterial-based therapeutics for antibiotic-resistant bacterial infections
Jessa Marie Makabenta,Ahmed Nabawy,Cheng-Hsuan Li,Suzannah M. Schmidt-Malan,Robin Patel,Vincent M. Rotello +5 more
TL;DR: The mechanisms by which nanomaterials can be used to target antibiotic-resistant bacterial infections are discussed, design elements and properties of nanomMaterials that can be engineered to enhance potency are highlighted, and recent progress and remaining challenges for clinical implementation are explored.
Journal ArticleDOI
Molecular mechanisms of antimicrobial tolerance and resistance in bacterial and fungal biofilms
TL;DR: Three mechanisms that play an important role in biofilm survival are discussed, found both in bacterial and fungal biofilms and are often surprisingly similar between distantly related organisms.
References
More filters
Journal ArticleDOI
Aminoglycoside antibiotics induce bacterial biofilm formation
Lucas R. Hoffman,David A. D'Argenio,Michael J. MacCoss,Zhaoying Zhang,Roger A. Jones,Samuel I. Miller +5 more
TL;DR: It is shown that subinhibitory concentrations of aminoglycoside antibiotics induce biofilm formation in P. aeruginosa and Escherichia coli, and the molecular basis of this response includes alterations in the level of c-di-GMP.
Journal ArticleDOI
A genetic basis for Pseudomonas aeruginosa biofilm antibiotic resistance
Thien-Fah Mah,Betsey Pitts,Brett J. Pellock,Brett J. Pellock,Graham C. Walker,Philip S. Stewart,George A. O'Toole +6 more
TL;DR: The results indicate that biofilms themselves are not simply a diffusion barrier to these antibiotics, but rather that bacteria within these microbial communities employ distinct mechanisms to resist the action of antimicrobial agents.
Journal ArticleDOI
Comprehensive transposon mutant library of Pseudomonas aeruginosa.
Michael A. Jacobs,Ashley Alwood,Iyarit Thaipisuttikul,David H. Spencer,Eric Haugen,Stephen Ernst,Oliver Will,Rajinder Kaul,Christopher K. Raymond,Ruth Levy,Liu Chun-Rong,Donald Guenthner,Donald Bovee,Maynard V. Olson,Colin Manoil +14 more
TL;DR: P phenotypic analysis of the collection may produce essentially complete lists of genes required for diverse biological activities, as well as facilitate downstream studies of gene expression, protein localization, epistasis, and chromosome engineering.
Journal ArticleDOI
Diffusion in biofilms
TL;DR: The profound influence of the physics of the diffusion process on the chemistry and biology of the biofilm mode of growth is discussed.
Journal ArticleDOI
Persister cells and tolerance to antimicrobials.
TL;DR: The data indicate that persisters are specialized survivor cells that are not at a particular stage in the cell cycle, neither are they defective cells nor cells created in response to antibiotics.