The extracellular matrix protects Pseudomonas aeruginosa biofilms by limiting the penetration of tobramycin.
Boo Shan Tseng,Wei Zhang,Joe J. Harrison,Tam P. Quach,Jisun L. Song,Jon Penterman,Jon Penterman,Pradeep K. Singh,David L. Chopp,Aaron I. Packman,Matthew R. Parsek +10 more
TLDR
It is proposed that tobramycin sequestration at the biofilm periphery is an important mechanism in protecting metabolically active cells that lie just below the zone of sequestration.Abstract:
Biofilm cells are less susceptible to antimicrobials than their planktonic counterparts. While this phenomenon is multifactorial, the ability of the matrix to reduce antibiotic penetration into the biofilm is thought to be of limited importance studies suggest that antibiotics move fairly rapidly through biofilms. In this study, we monitored the transport of two clinically relevant antibiotics, tobramycin and ciprofloxacin, into non-mucoid Pseudomonas aeruginosa biofilms. To our surprise, we found that the positively charged antibiotic tobramycin is sequestered to the biofilm periphery, while the neutral antibiotic ciprofloxacin readily penetrated. We provide evidence that tobramycin in the biofilm periphery both stimulated a localized stress response and killed bacteria in these regions but not in the underlying biofilm. Although it is unclear which matrix component binds tobramycin, its penetration was increased by the addition of cations in a dose-dependent manner, which led to increased biofilm death. These data suggest that ionic interactions of tobramycin with the biofilm matrix limit its penetration. We propose that tobramycin sequestration at the biofilm periphery is an important mechanism in protecting metabolically active cells that lie just below the zone of sequestration.read more
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Thymus vulgaris essential oil + tobramycin within nanostructured archaeolipid carriers: A new approach against Pseudomonas aeruginosa biofilms.
Noelia Molinero Pérez,María Julia Altube,Leandro R.S. Barbosa,Eder Lilia Romero,Ana Paula Perez +4 more
TL;DR: In this article , a co-delivery of Thymus vulgaris essential oil (EOT) and tobramycin (TB) by nanostructured archaeolipids carriers (NAC) could support nebulization and improve EOT and TB antioxidant, anti-inflammatory and antibiofilm activity.
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Pseudomonas aeuroginosa Biofilm and Antimicrobial Resistance
TL;DR: Biofilm caused by Increasing drug resistance in the world, as β-lactam antibiotics, will be caused a creating complication of P.a infection treatment, as biofilm can form antibiotic-resistant strains and increases many infections in the future.
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Multidimensional Clinical Surveillance of Pseudomonas aeruginosa Reveals Complex Relationships between Isolate Source, Morphology, and Antimicrobial Resistance
Laura J. Dunphy,Glynis L. Kolling,Matthew L. Jenior,Joanne Carroll,April E Attai,Farzad Farnoud,Amy J. Mathers,Molly A. Hughes,Jason A. Papin +8 more
TL;DR: Novel relationships between antimicrobial resistance, virulence-linked morphologies, and isolate source in a large and variable collection of clinical P. aeruginosa isolates are discovered, providing a potential guide for future antimicrobial treatment strategies.
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Antibiotic Cycling Affects Resistance Evolution Independently of Collateral Sensitivity
Pauline Brepoels,Kenny Appermans,Camilo Andres Perez-Romero,Bram Lories,Kathleen Marchal,Hans Steenackers +5 more
TL;DR: In this article , the effects of the evolved genetic background on the evolution of antibiotic resistance against Salmonella typhimurium were investigated by focusing on the first switch between two antibiotics.
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