The extracellular matrix protects Pseudomonas aeruginosa biofilms by limiting the penetration of tobramycin.
Boo Shan Tseng,Wei Zhang,Joe J. Harrison,Tam P. Quach,Jisun L. Song,Jon Penterman,Jon Penterman,Pradeep K. Singh,David L. Chopp,Aaron I. Packman,Matthew R. Parsek +10 more
TLDR
It is proposed that tobramycin sequestration at the biofilm periphery is an important mechanism in protecting metabolically active cells that lie just below the zone of sequestration.Abstract:
Biofilm cells are less susceptible to antimicrobials than their planktonic counterparts. While this phenomenon is multifactorial, the ability of the matrix to reduce antibiotic penetration into the biofilm is thought to be of limited importance studies suggest that antibiotics move fairly rapidly through biofilms. In this study, we monitored the transport of two clinically relevant antibiotics, tobramycin and ciprofloxacin, into non-mucoid Pseudomonas aeruginosa biofilms. To our surprise, we found that the positively charged antibiotic tobramycin is sequestered to the biofilm periphery, while the neutral antibiotic ciprofloxacin readily penetrated. We provide evidence that tobramycin in the biofilm periphery both stimulated a localized stress response and killed bacteria in these regions but not in the underlying biofilm. Although it is unclear which matrix component binds tobramycin, its penetration was increased by the addition of cations in a dose-dependent manner, which led to increased biofilm death. These data suggest that ionic interactions of tobramycin with the biofilm matrix limit its penetration. We propose that tobramycin sequestration at the biofilm periphery is an important mechanism in protecting metabolically active cells that lie just below the zone of sequestration.read more
Citations
More filters
Journal ArticleDOI
Extracellular Polymeric Substances (EPS) produced by Streptomyces sp. biofilms: Chemical composition and anticancer properties
TL;DR: In this paper, four Streptomyces strains isolated from contaminated soil (M7, A5, A14 and MC1) were evaluated for determining its biofilm-forming capacity including under pesticide stress.
DissertationDOI
Metabólitos secundários produzidos por fungos endofíticos isolados de Anthurium alcatrazense e Begonia spp.
TL;DR: O proteassoma como alvo terapêutico e metabólitos fúngicos ativos contra o proteassomas no combate aos biofilmes .............................................................................................................. 69
Journal ArticleDOI
Antimicrobial peptides from echinoderms as antibiofilm agents: a natural strategy to combat bacterial infections
TL;DR: The most recent studies on AMPs isolated from echinoderms show a great potential for application of AMPs in biotechnology for developing novel therapeutic agents that are either alternative or complementary to conventional antibiotic therapy to combat multi-resistant pathogens.
Journal ArticleDOI
The Natural Alkaloid Berberine Can Reduce the Number of Pseudomonas aeruginosa Tolerant Cells
Gianmarco Mangiaterra,Nicholas Cedraro,Emiliano Laudadio,Cristina Minnelli,Barbara Citterio,Francesca Andreoni,Giovanna Mobbili,Roberta Galeazzi,Francesca Biavasco +8 more
TL;DR: In this article, the effect of berberine on the MexXY-OprM efflux pump was investigated for the treatment of Pseudomonas aeruginosa (PA) lung infection in CF patients.
Journal ArticleDOI
Characterization of microbial colonization and diversity in reverse osmosis membrane autopsy
Sergio Martínez-Campos,Miguel Redondo-Nieto,Juan Shang,Nuria Peña,Francisco Leganés,Roberto Rosal,Francisca Fernández-Piñas +6 more
TL;DR: In this article, a three-tiered approach was proposed to identify key microorganisms (ateria and fungi) as biofilm formers and the extent of the biofilm matrix; this knowledge may be useful for new antifo uling treatments.
References
More filters
Journal ArticleDOI
Bacterial biofilms : A common cause of persistent infections
TL;DR: Improvements in understanding of the genetic and molecular basis of bacterial community behavior point to therapeutic targets that may provide a means for the control of biofilm infections.
Journal ArticleDOI
A broad host range mobilization system for in vivo genetic engineering: transposon mutagenesis in Gram negative bacteria
TL;DR: In this paper, a new vector strategy for the insertion of foreign genes into the genomes of gram negative bacteria not closely related to Escherichia coli was developed, which can utilize any gram negative bacterium as a recipient for conjugative DNA transfer.
Journal ArticleDOI
The Calgary Biofilm Device: New Technology for Rapid Determination of Antibiotic Susceptibilities of Bacterial Biofilms
TL;DR: Minimal biofilm eradication concentrations, derived by using the Calgary Biofilm Device, demonstrated that for biofilms of the same organisms, 100 to 1,000 times the concentration of a certain antibiotic were often required for the antibiotic to be effective, while other antibiotics were found to beeffective at the MICs.
Journal ArticleDOI
Common virulence factors for bacterial pathogenicity in plants and animals
Laurence G. Rahme,Emily J. Stevens,Sean F. Wolfort,Jing Shao,Ronald G. Tompkins,Frederick M. Ausubel +5 more
TL;DR: A Pseudomonas aeruginosa strain (UCBPP-PA14) is infectious both in an Arabidopsis thaliana leaf infiltration model and in a mouse full-thickness skin burn model, indicating that these genes encode virulence factors required for the full expression of pathogenicity in both plants and animals.
Journal ArticleDOI
Mechanisms of antibiotic resistance in bacterial biofilms.
TL;DR: Disabling biofilm resistance may enhance the ability of existing antibiotics to clear infections involving biofilms that are refractory to current treatments.