The genome sequence of the food-borne pathogen Campylobacter jejuni reveals hypervariable sequences
Julian Parkhill,Brendan W. Wren,Karen Mungall,Julian M. Ketley,Carol Churcher,D. Basham,Tracey Chillingworth,Robert L. Davies,Theresa Feltwell,S. Holroyd,Kay Jagels,Andrey V. Karlyshev,Sharon Moule,Mark J. Pallen,Charles W. Penn,Michael A. Quail,Marie-Adèle Rajandream,Kim Rutherford,A. H. M. van Vliet,Sally Whitehead,Bart Barrell +20 more
TLDR
The genome sequence of C. jejuni NCTC11168 is reported, finding short homopolymeric runs of nucleotides were commonly found in genes encoding the biosynthesis or modification of surface structures, or in closely linked genes of unknown function.Abstract:
Campylobacter jejuni, from the delta-epsilon group of proteobacteria, is a microaerophilic, Gram-negative, flagellate, spiral bacterium—properties it shares with the related gastric pathogen Helicobacter pylori. It is the leading cause of bacterial food-borne diarrhoeal disease throughout the world1. In addition, infection with C. jejuni is the most frequent antecedent to a form of neuromuscular paralysis known as Guillain–Barre syndrome2. Here we report the genome sequence of C. jejuni NCTC11168. C. jejuni has a circular chromosome of 1,641,481 base pairs (30.6% G+C) which is predicted to encode 1,654 proteins and 54 stable RNA species. The genome is unusual in that there are virtually no insertion sequences or phage-associated sequences and very few repeat sequences. One of the most striking findings in the genome was the presence of hypervariable sequences. These short homopolymeric runs of nucleotides were commonly found in genes encoding the biosynthesis or modification of surface structures, or in closely linked genes of unknown function. The apparently high rate of variation of these homopolymeric tracts may be important in the survival strategy of C. jejuni.read more
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Artemis: sequence visualization and annotation.
Kim Rutherford,Julian Parkhill,James Crook,Terry Horsnell,Peter M. Rice,Marie-Adèle Rajandream,Bart Barrell +6 more
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An Eulerian path approach to DNA fragment assembly
TL;DR: This work abandons the classical “overlap–layout–consensus” approach in favor of a new euler algorithm that, for the first time, resolves the 20-year-old “repeat problem” in fragment assembly.
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Campylobacter jejuni Infections: Update on Emerging Issues and Trends
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Classification and phylogeny of hydrogenases.
TL;DR: Compelling evidence from sequences and structures indicates that the [NiFe]- and [Fe]-H2ases are phylogenetically distinct classes of proteins, which would be consistent with the phylogenetic distinctiveness of the two classes of H2ases.
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Guillain-Barré syndrome.
TL;DR: The data for GBS suggests that the immunologic mechanism can involve molecular mimicry, at least in some GBS variants, and it is likely that multiple mechanisms render the axon vulnerable.
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