The high-affinity binding of Clostridium botulinum type B neurotoxin to synaptotagmin II associated with gangliosides GT1b/GD1a
Teiichi Nishiki,Yoshimi Tokuyama,Yoichi Kamata,Yasuo Nemoto,Akira Yoshida,Kazuki Sato,Mariko Sekiguchi,Masami Takahashi,Shunji Kozaki +8 more
Reads0
Chats0
TLDR
Results suggest that this region of synaptotagmin II participates in the formation of the high‐affinity toxin binding site by associating with specific gangliosides.About:
This article is published in FEBS Letters.The article was published on 1996-01-15 and is currently open access. It has received 228 citations till now. The article focuses on the topics: Synaptotagmin I & Synaptotagmin 1.read more
Citations
More filters
Journal ArticleDOI
Neurotoxins Affecting Neuroexocytosis
TL;DR: The mechanism of action of three groups of presynaptic neurotoxins that interfere directly with the process of neurotransmitter release is reviewed, whereas presynapses acting on ion channels are not dealt with here.
Journal ArticleDOI
Crystal structure of botulinum neurotoxin type A and implications for toxicity.
TL;DR: The crystal structure of the entire 1,285 amino acid di-chain neurotoxin was determined and the toxin appears as a hybrid of varied structural motifs and suggests a modular assembly of functional subunits to yield pathogenesis.
Journal ArticleDOI
SV2 is the protein receptor for botulinum neurotoxin A.
Min Dong,Felix L. Yeh,William H. Tepp,Camin Dean,Eric A. Johnson,Roger Janz,Edwin R. Chapman +6 more
TL;DR: It is found that BoNT/A enters neurons by binding to the synaptic vesicle protein SV2 (isoforms A, B, and C), and SV2 acts as the protein receptor for Bo NT/A.
Journal ArticleDOI
Sphingolipids in Food and the Emerging Importance of Sphingolipids to Nutrition
Hubert W. Vesper,Eva-Maria Schmelz,Mariana Nikolova-Karakashian,Dirck L. Dillehay,Daniel V. Lynch,Alfred H. Merrill +5 more
TL;DR: There is no known nutritional requirement for sphingolipids; nonetheless, they are hydrolyzed throughout the gastrointestinal tract to the same categories of metabolites that are used by cells to regulate growth, differentiation, apoptosis and other cellular functions.
Journal ArticleDOI
Identification of the Major Steps in Botulinum Toxin Action
TL;DR: This review seeks to identify and characterize all major steps in toxin action, from initial absorption to eventual paralysis of cholinergic transmission, as well as an agent that can be used to treat disease.
References
More filters
Journal ArticleDOI
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
Journal ArticleDOI
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
Journal Article
Cleavage of structural proteins during the assemble of the head of bacterio-phage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products as mentioned in this paper.
Journal ArticleDOI
Quantitative estimation of sialic acids. II. A colorimetric resorcinol-hydrochloric acid method.
TL;DR: A new method for the quantitive determination of sialic acids is described, which is about 50% more sensitive than the orcinol-hydrochloric acid method generally used and considerably lower with the resorcin reagent.
Journal ArticleDOI
The synaptic vesicle cycle: a cascade of protein–protein interactions
TL;DR: A convergence of results now allows formulation of molecular models for key steps of the synaptic vesicle cycle, which may form the basis for a mechanistic understanding of higher neural function.