The Phosphatase Cdc14 Triggers Mitotic Exit by Reversal of Cdk-Dependent Phosphorylation
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TLDR
This work shows that the Cdc14 phosphatase triggers mitotic exit by three parallel mechanisms, each of which inhibits Cdk activity, and induces degradation of mitotic cyclins.About:
This article is published in Molecular Cell.The article was published on 1998-12-01 and is currently open access. It has received 780 citations till now. The article focuses on the topics: Mitotic exit & Polo-like kinase.read more
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Dissertation
Exploring the roles of the C-terminal of Pol2, the catalytic subunit of DNA Polymerase ɛ, in replication and checkpoint response
TL;DR: In this article, expression of the last 236 residues of a truncation mutant, pol2-11, was shown to partially suppress the defects in origin firing, fork progression and checkpoint signaling inherent to a truncated mutant, and conserved residues were identified for suppressive effects of the C-terminal fragment.
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Reduced Cdc14 phosphatase activity impairs septation, hyphal differentiation and pathogenesis and causes echinocandin hypersensitivity in Candida albicans
Kedric L. Milholland,Ahmed Abdelkhalek,Kortany M. Baker,Smriti Hoda,Andrew G. DeMarco,Noelle H. Naughton,Angela N. Koeberlein,Gabrielle Renee Lorenz,Kartikan Anandasothy,Antonio Esperilla-Muñoz,Sanjeev K. Narayanan,Jaime Correa-Bordes,Scott D. Briggs,Mark C. Hall +13 more
TL;DR: A system to test if partial therapeutic reduction in activity of a protein phosphatase called Cdc14 could reduce virulence of the opportunistic human pathogen Candida albicans found that successful C.Albicans infections in two animal models of invasive candidiasis were dependent on high CDC14 activity.
Journal ArticleDOI
Profile of Angelika Amon, winner of the 2019 Vilcek Prize in Biomedical Science.
Jan Vilcek,Prashant Nair +1 more
TL;DR: The recipient of the 2019 Vilcek Prize in Biomedical Science is Angelika Amon, an Austrian-born molecular and cell biologist at the Massachusetts Institute of Technology.
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Cdc14 phosphatase ensures resolution of aberrant meiotic recombination outcomes partly through activating Yen1/GEN1
Carolina Pascual-Silva,Katerina Strouhalova,George B. Garside,Meret Arter,David Álvarez-Melo,Teresa Bermejo,Pedro A. San-Segundo,Joao Matos,Marco Geymonat,Jesús A. Carballo +9 more
TL;DR: It is found that levels of aberrant recombination intermediates accumulate during prophase I in cdc14 meiotic deficient cells, and an early function for Cdc14 in meiotic recombination independent of its later roles during anaphase I/II is proposed.
Journal ArticleDOI
Cdc14 phosphatase contributes to cell wall integrity and pathogenesis in Candida albicans
Kedric L. Milholland,Ahmed Abdelkhalek,Kortany M. Baker,Smriti Hoda,Andrew G. DeMarco,Noelle H. Naughton,Angela N. Koeberlein,Gabrielle Renee Lorenz,Kartikan Anandasothy,Antonio Esperilla-Muñoz,Sanjeev K. Narayanan,Jaime Correa-Bordes,Scott D. Briggs,Mark C. Hall +13 more
TL;DR: In this article , an invariant motif was identified in the disordered C-terminal tail of fungal Cdc14 enzymes that is required for full enzyme activity, and a reduced-activity hypomorphic mutant allele (cdc14hm) was shown to severely impair C. albicans virulence in both assays.
References
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Journal ArticleDOI
Cyclin-dependent kinases: engines, clocks, and microprocessors.
TL;DR: This work has shown that Cdk activity is governed by a complex network of regulatory subunits and phosphorylation events whose precise effects on Cdk conformation have been revealed by recent crystallographic studies.
Journal ArticleDOI
SKP1 connects cell cycle regulators to the ubiquitin proteolysis machinery through a novel motif, the F-box.
TL;DR: Different skp1 mutants arrest cells in either G1 or G2, suggesting a connection between regulation of proteolysis in different stages of the cycle.
Journal ArticleDOI
How proteolysis drives the cell cycle
TL;DR: Proteolysis drives cell cycle progression not only by regulating CDK activity, but by directly influencing chromosome and spindle dynamics, and also how proteolysis may directly trigger the transition from metaphase to anaphase.
Journal ArticleDOI
F-Box Proteins Are Receptors that Recruit Phosphorylated Substrates to the SCF Ubiquitin-Ligase Complex
TL;DR: The ubiquitination pathway for the Cdk inhibitor Sic1 is reconstituted using recombinant proteins and the constituents of the SCF complex are members of protein families, likely to serve as the prototype for a large class of E3s formed by combinatorial interactions of related family members.
Journal ArticleDOI
The Saccharomyces cerevisiae Cell Cycle
TL;DR: The bibliography is intended more as a guide to the literature than as a historically accurate record of the development of the field; the authors apologize to the earlier workers whose contributions thus get less explicit credit than they deserve.