Journal ArticleDOI
The pivotal role of microRNA-21 in osteoclastogenesis inhibition by anthracycline glycoside aloin.
Radha Madhyastha,Harishkumar Madhyastha,Yutthana Pengjam,Queen Intan Nurrahmah,Yuichi Nakajima,Masugi Maruyama +5 more
TLDR
In this paper, the role of miR-21 in aloin's inhibitory effect on osteoclast differentiation was investigated and it was shown that miR21 suppression resulted in upregulation of osteoclastic suppressor programmed cell death protein 4 (PDCD4) and downregulation of OCC marker cathepsin K.Abstract:
Osteopenic disorders such as osteoporosis and rheumatoid arthritis are characterized by excessive bone resorption by osteoclasts relative to bone formation by osteoblasts. MicroRNAs are emerging as key players in bone remodeling, modulating the functions of both osteoblasts and osteoclasts. Among them, miR-21 is highly expressed in osteoclast precursors and is known to regulate genesis, differentiation, and apoptosis of osteoclasts. The pro-osteoclastogenic nature of miR-21 makes it a potential candidate as a therapeutic target to treat bone disorders. We had previously demonstrated that anthroglycoside aloin derived from Aloe vera was effective in promoting osteoblastogenesis and inhibiting osteoclastogenesis. The present study investigated the role of miR-21 in aloin’s inhibitory effect on osteoclast differentiation. Aloin effectively suppressed receptor activator of nuclear factor kappa-B (NFĸB) ligand (RankL)-induced miR-21 expression via repression of NFĸB activation. MiR-21 suppression resulted in upregulation of osteoclast suppressor programmed cell death protein 4 (PDCD4), and downregulation of osteoclast marker cathepsin K. Knockdown or gain-of-function studies revealed that miR-21 was pivotal to aloin’s inhibitory effect on osteoclastogenesis. This study also highlights the dynamic potential of aloin as a therapeutic agent to treat osteopenic disorders.read more
Citations
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Journal ArticleDOI
Pharmacological Update Properties of Aloe Vera and its Major Active Constituents.
TL;DR: New pharmacological data research has shown that most studies refer to anti-cancer action, skin and digestive protective activity, and antimicrobial properties, and most recent works are in vitro and in vivo.
Journal ArticleDOI
The roles of miRNA, lncRNA and circRNA in the development of osteoporosis
TL;DR: The results of recent studies on the mechanisms of miRNA-, lncRNA-, and circRNA-mediated osteoporosis associated with osteoblasts and osteoclasts are summarized.
Journal ArticleDOI
Circulating miR-181c-5p and miR-497-5p Are Potential Biomarkers for Prognosis and Diagnosis of Osteoporosis.
Ma Jianhua,Xiao Lin,Chu Chen,Li Siyu,Shasha Zhang,Zhihao Chen,Dijie Li,Fan Zhao,Chaofei Yang,Chong Yin,Wuxia Qiu,Yunyun Xiao,Kewen Zhang,Zhiping Miao,Tuanmin Yang,Airong Qian +15 more
TL;DR: MiR-181c- 5p and miR-497-5p are involved in bone metabolism and associated with progressive bone loss of osteoporosis, suggesting that circulating miRNAs might act as potential biomarkers for monitoring effects of anti-osteoporotic therapies or diagnostic approach.
Journal ArticleDOI
MicroRNA 21 Elicits a Pro-inflammatory Response in Macrophages, with Exosomes Functioning as Delivery Vehicles.
Radha Madhyastha,Harishkumar Madhyastha,Queen Intan Nurrahmah,Bethasiwi Purbasari,Masugi Maruyama,Yuichi Nakajima +5 more
TL;DR: In this paper, exosomes from transfected cells were used to study the paracrine action of miR-21 on naive macrophages, and they showed that the exosomal miR21-rich exosome can be used as a biomarker and therapeutic target in inflammatory conditions.
Journal ArticleDOI
MicroRNA-21 affects mechanical force-induced midpalatal suture remodelling.
TL;DR: This study aimed to elucidate the effects of miR‐21 on the midpalatal suture bone remodelling by expanding the palatal sutures.
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