The replication fork trap and termination of chromosome replication.
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TLDR
This work reviews the research that led to the replication fork trap theory, and aims to integrate several recent findings that contribute towards an understanding of the physiological roles of the replication forks trap.Abstract:
Bacteria that have a circular chromosome with a bidirectional DNA replication origin are thought to utilize a 'replication fork trap' to control termination of replication. The fork trap is an arrangement of replication pause sites that ensures that the two replication forks fuse within the terminus region of the chromosome, approximately opposite the origin on the circular map. However, the biological significance of the replication fork trap has been mysterious, as its inactivation has no obvious consequence. Here we review the research that led to the replication fork trap theory, and we aim to integrate several recent findings that contribute towards an understanding of the physiological roles of the replication fork trap. Likely roles include the prevention of over-replication, and the optimization of post-replicative mechanisms of chromosome segregation, such as that involving FtsK in Escherichia coli.read more
Citations
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Replication fork reversal in eukaryotes: from dead end to dynamic response.
Kai J. Neelsen,Massimo Lopes +1 more
TL;DR: The study of the putative in vivo roles of recently identified eukaryotic factors in fork remodelling promises to shed new light on mechanisms of genome maintenance and to provide novel attractive targets for cancer therapy.
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Organization and segregation of bacterial chromosomes
TL;DR: It is argued that the key feature of compaction is the orderly folding of DNA along adjacent segments and that this organization provides easy and efficient access for protein–DNA transactions and has a central role in driving segregation.
Journal ArticleDOI
Replication Termination at Eukaryotic Chromosomes Is Mediated by Top2 and Occurs at Genomic Loci Containing Pausing Elements
Daniele Fachinetti,Rodrigo Bermejo,Andrea Cocito,Simone Minardi,Yuki Katou,Yutaka Kanoh,Katsuhiko Shirahige,Anna Azvolinsky,Virginia A. Zakian,Marco Foiani +9 more
TL;DR: It is proposed that in eukaryotes, replication fork barriers, Rrm3, and Top2 coordinate replication fork progression and fusion at TERs, thus counteracting abnormal genomic transitions.
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Mechanisms of DNA replication termination
TL;DR: The recently discovered E3 ubiquitin ligases that control replisome disassembly in yeast and higher eukaryotes are discussed, and how their activity is regulated to avoid genome instability.
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Is RecG a general guardian of the bacterial genome
TL;DR: It is concluded that RecG may be both a specialist activity and a general guardian of the genome through the ability of RecG to eliminate substrates that the replication restart protein, PriA, could otherwise exploit to re-replicate the chromosome.
References
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