Tuft cells are key mediators of interkingdom interactions at mucosal barrier surfaces
Madison S. Strine,Craig B. Wilen +1 more
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TLDR
A comprehensive overview of microbial activation of tuft cells with an emphasis onTuft cell heterogeneity and differences between mouse and human tuft cell biology as it pertains to human health and disease is provided.Abstract:
Although tuft cells were discovered over 60 years ago, their functions have long been enigmatic, especially in human health. Nonetheless, tuft cells have recently emerged as key orchestrators of the host response to diverse microbial infections in the gut and airway. While tuft cells are epithelial in origin, they exhibit functions akin to immune cells and mediate important interkingdom interactions between the host and helminths, protists, viruses, and bacteria. With broad intra- and intertissue heterogeneity, tuft cells sense and respond to microbes with exquisite specificity. Tuft cells can recognize helminth and protist infection, driving a type 2 immune response to promote parasite expulsion. Tuft cells also serve as the primary physiologic target of persistent murine norovirus (MNV) and promote immune evasion. Recently, tuft cells were also shown to be infected by rotavirus. Other viral infections, such as influenza A virus, can induce tuft cell–dependent tissue repair. In the context of coinfection, tuft cells promote neurotropic flavivirus replication by dampening antiviral adaptive immune responses. Commensal and pathogenic bacteria can regulate tuft cell abundance and function and, in turn, tuft cells are implicated in modulating bacterial infiltration and mucosal barrier integrity. However, the contribution of tuft cells to microbial sensing in humans and their resulting effector responses are poorly characterized. Herein, we aim to provide a comprehensive overview of microbial activation of tuft cells with an emphasis on tuft cell heterogeneity and differences between mouse and human tuft cell biology as it pertains to human health and disease.read more
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Tuft-cell-intrinsic and -extrinsic mediators of norovirus tropism regulate viral immunity
Madison S. Strine,Mia Madel Alfajaro,Vincent R. Graziano,Jaewon Song,Leon L. Hsieh,Ryan Hill,Jun Guo,Kelli L. VanDussen,Robert C. Orchard,Megan T. Baldridge,Sanghyun Lee,Craig B. Wilen +11 more
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Raptor knockdown concurrently increases the electrical resistance and paracellular permeability of Caco-2 cell monolayers.
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NAIP—NLRC4 Inflammasome Activation in Tuft Cells Activates a PGD2–ILC3 Signaling Circuit that Protects Against Enteric Infection
Madeline J Churchill,Renate Bauer,T. Hanson Christopher,Shuchi Smita,Lindsey M. Warner,Bridget Mooney,Elia D. Tait Wojno,Isabella Rauch +7 more
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Interkingdom Detection of Bacterial Quorum-Sensing Molecules by Mammalian Taste Receptors
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TL;DR: In this paper , a role for indoleamine 2,3-dioxygenase 1 (Ido1) in the cellular tropism of murine and human astroviruses was identified.
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TL;DR: ‘pulse-seq’ is developed, combining scRNA-seq and lineage tracing, to show that tuft, neuroendocrine and ionocyte cells are continually and directly replenished by basal progenitor cells, establishing a new cellular narrative for airways disease.
Journal ArticleDOI
Intestinal epithelial tuft cells initiate type 2 mucosal immunity to helminth parasites
François Gerbe,François Gerbe,François Gerbe,Emmanuelle Sidot,Emmanuelle Sidot,Emmanuelle Sidot,Danielle J. Smyth,Makoto Ohmoto,Ichiro Matsumoto,Valerie Dardalhon,Valerie Dardalhon,Pierre Cesses,Pierre Cesses,Pierre Cesses,Laure Garnier,Laure Garnier,Laure Garnier,Marie Pouzolles,Marie Pouzolles,Bénédicte Brulin,Bénédicte Brulin,Bénédicte Brulin,Marco Bruschi,Marco Bruschi,Marco Bruschi,Yvonne Harcus,Valérie S. Zimmermann,Valérie S. Zimmermann,Naomi Taylor,Naomi Taylor,Rick M. Maizels,Philippe Jay,Philippe Jay,Philippe Jay +33 more
TL;DR: It is shown that tuft cells, a rare epithelial cell type in the steady-state intestinal epithelium, are responsible for initiating type 2 responses to parasites by a cytokine-mediated cellular relay and a cellular relay required for initiating mucosal type 2 immunity to helminth infection.