Journal ArticleDOI
Ubiquitination of histone H2B regulates H3 methylation and gene silencing in yeast
Zu-Wen Sun,C. David Allis +1 more
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TLDR
It is shown that the ubiquitin-conjugating enzyme Rad6 (Ubc2) mediates methylation of histone H3 at lysine 4 (Lys 4) through ubiquitination of H2B at Lys 123 in yeast (Saccharomyces cerevisiae) to reveal a pathway leading to gene regulation through concerted histone modifications on distinct histone tails.Abstract:
In eukaryotes, the DNA of the genome is packaged with histone proteins to form nucleosomal filaments, which are, in turn, folded into a series of less well understood chromatin structures. Post-translational modifications of histone tail domains modulate chromatin structure and gene expression. Of these, histone ubiquitination is poorly understood. Here we show that the ubiquitin-conjugating enzyme Rad6 (Ubc2) mediates methylation of histone H3 at lysine 4 (Lys 4) through ubiquitination of H2B at Lys 123 in yeast (Saccharomyces cerevisiae). Moreover, H3 (Lys 4) methylation is abolished in the H2B-K123R mutant, whereas H3-K4R retains H2B (Lys 123) ubiquitination. These data indicate a unidirectional regulatory pathway in which ubiquitination of H2B (Lys 123) is a prerequisite for H3 (Lys 4) methylation. We also show that an H2B-K123R mutation perturbs silencing at the telomere, providing functional links between Rad6-mediated H2B (Lys 123) ubiquitination, Set1-mediated H3 (Lys 4) methylation, and transcriptional silencing. Thus, these data reveal a pathway leading to gene regulation through concerted histone modifications on distinct histone tails. We refer to this as 'trans-tail' regulation of histone modification, a stated prediction of the histone code hypothesis.read more
Citations
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The Gene Encoding Human SCGB 2A1 is under Indirect Androgen Control Operating through an Sp Family Binding Site in Prostate Cells
TL;DR: Because expression was found to be under androgen control, gene regulation was investigated using the androgen responsive human prostate cancer cell line LNCaP and it was found that androgen responsiveness in the SCGB 2A1 gene is mediated by an indirect androgen response.
Dissertation
Epigenetic regulation of developmental genes in embryonic stem cell differentation
TL;DR: The epigenetica, a process that conduce to the formación of an organismo completeto a partir of a single celula mediante cambios progresivos, was defined by Conrad Waddington in 1942.
Book ChapterDOI
Chapter 292 – Histone Ubiquitination
Vikki M. Weake,Jerry L. Workman +1 more
TL;DR: In this paper, it was shown that the enzymes mediating both the addition and removal of ubiquitin from histones H2A and H2B tend to reside within large multi-subunit complexes that often contain additional catalytic activities.
Dissertation
Characterization of Rpn10 monoubiquitination as a novel way of proteasome regulation
TL;DR: It is shown that Rpn10 is monoubiquitinated (Rpn10-mUb) in vivo and that RPN10- mUb is found in both proteasomal and non-proteasomal pools, and suggested that in an extraprotesasomal context, Rpn 10 monoubquitination could regulate the Dsk2-Rpn 10 interaction.
Dissertation
Analysis of the effects of spatial localisation of transgenes on expression of recombinant proteins in CHO-DG44 cells
TL;DR: This project is based on the hypothesis that certain locations within the three-dimensional structure of the nucleus are more favourable for stable high-level expression of recombinant genes, integration of transgene into these specific location will produce high expressing stable cell lines.
References
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Journal ArticleDOI
Translating the Histone Code
Thomas Jenuwein,C. David Allis +1 more
TL;DR: It is proposed that this epigenetic marking system represents a fundamental regulatory mechanism that has an impact on most, if not all, chromatin-templated processes, with far-reaching consequences for cell fate decisions and both normal and pathological development.
Journal ArticleDOI
The language of covalent histone modifications.
Brian D. Strahl,C D Allis +1 more
TL;DR: It is proposed that distinct histone modifications, on one or more tails, act sequentially or in combination to form a ‘histone code’ that is, read by other proteins to bring about distinct downstream events.
Journal ArticleDOI
Twenty-Five Years of the Nucleosome, Fundamental Particle of the Eukaryote Chromosome
Roger D. Kornberg,Yahli Lorch +1 more
TL;DR: The chromatin field needs much more information about structure beyond the nucleosome, and there is insufficient evidence that acetylation actually causes chromatin unfolding, and functional analysis in cell-free systems must be extended beyond theucleosome to the chromosomal context.
Journal ArticleDOI
Transcription regulation by histone methylation: interplay between different covalent modifications of the core histone tails
Yi Zhang,Danny Reinberg +1 more
TL;DR: This work aims to demonstrate the efforts towards in-situ applicability of EMMARM, which aims to provide real-time information about the “building blocks” of EMT and its role in cancer progression.
Journal ArticleDOI
Histone acetylation and an epigenetic code
TL;DR: Recent evidence raises the interesting possibility that an acetylation-based code may operate through both mitosis and meiosis, providing a possible mechanism for germ-line transmission of epigenetic changes.